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Igy neutralizing antibody against novel coronavirus and preparation method, preparation and application thereof

A coronavirus and antibody technology, applied in antiviral agents, chemical instruments and methods, antiviral immunoglobulins, etc., can solve the problems of no approved active immunization vaccines, no specific drugs, etc., and achieve excellent blocking of virus survival. , improve the defense effect, the effect of good stability

Active Publication Date: 2021-05-18
SHENZHEN HUARUI TONGKANG BIOTECHNOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, although great progress has been made in the development and treatment of new coronary pneumonia vaccines, there is still no specific drug, and there is no active immunization vaccine approved for marketing

Method used

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  • Igy neutralizing antibody against novel coronavirus and preparation method, preparation and application thereof
  • Igy neutralizing antibody against novel coronavirus and preparation method, preparation and application thereof
  • Igy neutralizing antibody against novel coronavirus and preparation method, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Design and screening of antigens

[0024] The present invention selects novel coronavirus spike protein extracellular region protein (S-ECD protein, GenBank accession number QHD43416.1, a.a.Val16-Pro1213) and spike protein receptor binding domain (RBD protein, GenBank accession number QHD43416.1 , a.a.Arg319-Phe541) as immunogen. Both S-ECD protein and RBD protein are recombinant proteins, purchased from Nanjing GenScript Biotechnology Co., Ltd.

Embodiment 2

[0025] Embodiment 2: Preparation of anti-new coronavirus IgY antibody

[0026]Use the new coronavirus spike protein extracellular domain (S-ECD protein) as the immunization antigen and mix it with Freund's adjuvant at a ratio of 1:1, prepare an emulsion, and inject subcutaneously at multiple sites to immunize poultry (such as hens) or For birds (such as female ostriches), after two immunizations, KLH-RBD is used for the third and subsequent booster immunization; the yolk liquid is extracted, and the antibody solution is prepared by water extraction, and the plate chemiluminescence ELISA coated with S-ECD or RBD is used After screening highly specific anti-new coronavirus IgY antibodies, the IgY antibodies were prepared.

Embodiment 3

[0027] Example 3: Screening of highly specific anti-new coronavirus IgY antibodies by plate chemiluminescence ELISA

[0028] Determination was carried out by plate chemiluminescent ELISA method (biotin-streptavidin-horseradish peroxidase system). Use the antibody concentration dilution gradient (100×, 200×, 400×, 800×, 1600×, 3200×, 6400×) screening method to immunize each immunized ostrich or chicken with an ELISA plate coated with ECD or RBD Primary screening and identification of effects. For the 15 candidate numbers obtained after the initial screening ( figure 1 Among them, immunization numbers 1, 3, and 5 are ostrich numbers; immunization numbers 6, 7, 91, 93, 94, 95, 96, 97, 98, 99, and 100 are hen numbers) IgY antibody solution, according to the above method Do a second screening. Screened immune response results such as figure 1 shown. After repeated measurements, it was found that Ostrich No. 1 and Chicken No. 7 had the best immune response specificity and sensi...

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Abstract

The present invention provides a novel coronavirus spike protein extracellular region (S-ECD protein) and spike protein receptor binding domain (RBD) to immunize poultry (hen) or birds (hen ostrich) to prepare highly efficient Anti-2019-nCoV-specific IgY neutralizing antibody. The present invention also provides anti-new coronavirus IgY antibody type I and type II preparations developed by the neutralizing antibody, including type I-inhalation atomized spray and infusion injection and type II-coating. The present invention also provides novel coronavirus prevention and treatment drugs comprising the above antibodies and preparations. The present invention further provides the application of the above-mentioned antibodies and preparations in drugs for the prevention and treatment of novel coronavirus.

Description

technical field [0001] The present invention relates to the application of IgY antibodies to prepare antiviral preparations, in particular to an anti-new coronavirus IgY neutralizing antibody, a preparation method, a new crown defense preparation, and their application. Background technique [0002] The novel coronavirus pneumonia (referred to as new coronary pneumonia, or COVID-19) epidemic is the most urgent acute infectious disease event in the world today, and it has posed a severe challenge to the global public health community. Novel coronavirus (referred to as new coronavirus, or SARS-CoV-2) has a large number of infected people, is highly transmissible, and has a high case fatality rate. So far, although great progress has been made in the development and treatment of new coronary pneumonia vaccines, there is still no specific drug, and there is no active immunization vaccine approved for marketing. Therefore, the control of the new crown pneumonia epidemic urgently...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C07K16/02A61K39/42A61K9/12A61K9/72A61K9/08A61K47/26A61K47/02A61K47/18A61K47/24A61P31/14
CPCA61K9/0014A61K9/0019A61K9/0073A61K9/08A61K9/12A61K47/02A61K47/183A61K47/186A61K47/24A61K47/26A61K2039/505A61P31/14C07K16/02C07K16/10C07K2317/11C07K2317/23C07K2317/76C07K2317/94
Inventor 李劲张波李惠军黑爱莲方聪谭俊烨高鹏艾伦·何斯文·斯库格周际
Owner SHENZHEN HUARUI TONGKANG BIOTECHNOLOGICAL
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