mRNA-liposome complex and application thereof

A technology of liposome complexes and liposomes, which can be used in liposome delivery, DNA/RNA vaccination, complete cell/virus/DNA/RNA components, etc., and can solve the problem of high toxicity
CN112107680AActive Publication Date: 2020-12-22ZHEJIANG ZHIDA PHARM CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG ZHIDA PHARM CO LTD
Publication Date
2020-12-22

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Abstract

The invention belongs to the field of bioengineering and medicine, and particularly relates to an mRNA-liposome complex for introducing mRNA with modification into cells as well as application, a preparation method and the like of the mRNA-liposome complex. The mRNA-liposome complex contains a transfection reagent and at least one mRNA with modification. Inconspicuously, the mRNA-liposome complexcan efficiently mediate in vivo transfection of mRNA to a plurality of organs in a topical delivery manner. According to the mRNA-liposome complex, intracellular delivery of modified mRNA, resulting in gene expression, can be used to study biological functions of specific genes, can also correct / treat diseases caused by gene deletion or low gene expression. In addition, the mRNA-liposome complex can be used as a nucleic acid vaccine to prevent infectious diseases and treat cancer. In addition, administration of the mRNA-liposome complex is very simple and convenient, and is safe and effective.
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Description

technical field

[0001] The invention belongs to the fields of bioengineering and medicine, and relates to an mRNA-liposome complex containing modified mRNA and liposomes and its application and preparation method. Background technique

[0002] At present, recombinant protein biopharmaceuticals are becoming more and more important in the application of disease treatment and prevention. It is a simple, effective and more economical method to introduce protein-coding DNA or RNA into cells through a suitable carrier to guide the synthesis of cellular proteins. Viral vectors and non-viral vectors have been developed to deliver DNA or RNA exogenous genes to cells. Viral vectors have a certain risk of mutation from replication-deficient to wild-type, and may even lead to mutagenesis of cells. The preparation of viral vectors is complex, tedious, and highly immunogenic [1]. Non-viral vectors represented by cationic liposomes and polymers deliver biologically active macromolecules...

Claims

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