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A chrysanthemum-like light-weight exclusionable protein molecularly imprinted magnetic nanomaterial and its preparation method and application

A magnetic nanometer and molecular imprinting technology, applied in chemical instruments and methods, ion exchange, other chemical processes, etc., can solve the problems of low estradiol adsorption, poor protein exclusion effect, and complicated synthesis of molecularly imprinted materials. Low material cost, strong selective adsorption capacity, and strong reusability

Active Publication Date: 2021-12-28
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to overcome the shortcomings of the prior art that the mass of the magnetic carrier is large, the synthesis of the molecularly imprinted material is cumbersome, and the exclusion effect on the protein in milk is poor, resulting in a low adsorption amount of estradiol, the purpose of the present invention is to provide a chrysanthemum-shaped light Exclusive protein molecularly imprinted magnetic nanomaterial and its preparation method and application

Method used

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  • A chrysanthemum-like light-weight exclusionable protein molecularly imprinted magnetic nanomaterial and its preparation method and application
  • A chrysanthemum-like light-weight exclusionable protein molecularly imprinted magnetic nanomaterial and its preparation method and application
  • A chrysanthemum-like light-weight exclusionable protein molecularly imprinted magnetic nanomaterial and its preparation method and application

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Effect test

Embodiment 1

[0031] A method for preparing a chrysanthemum-shaped lightweight protein molecularly imprinted magnetic nanomaterial, comprising the following steps:

[0032]Step 1. Put 0.6g of ferric chloride, 2.0g of anhydrous sodium acetate, 1.0g of PEG-400 and 20mL of ethylene glycol in a 100mL three-necked flask, and stir for 3 hours in an oil bath at 160°C at a speed of 50r / min. Then the reaction product was poured into a 50mL polytetrafluoroethylene reactor and reacted at 150°C for 2h. Finally, the product after the reaction was washed with ultrapure water until neutral, and vacuum-dried at 10°C and 0.01MPa for 1h. The chrysanthemum-like lightweight magnetic nanospheres were prepared. Such as figure 1 As shown, its particle size is about 150nm.

[0033] Step 2. Place 20mg of chrysanthemum-shaped lightweight magnetic nanospheres, 10mg of estradiol, 10mg of bovine serum albumin, 1.0g of tris(2-carbonylethyl)phosphate hydrochloride and 20mL of phosphate buffer into a 100mL three-necked ...

Embodiment 2

[0044] A method for preparing a chrysanthemum-shaped lightweight protein molecularly imprinted magnetic nanomaterial, comprising the following steps:

[0045] Step 1. Put 0.96g of ferric chloride, 3.5g of anhydrous sodium acetate, 2.0g of PEG-400 and 30mL of ethylene glycol in a 100mL three-necked flask, and stir for 4 hours in an oil bath at 180°C at a speed of 100r / min. Then the reaction product was poured into a 50mL polytetrafluoroethylene reactor and reacted at 160°C for 4h. Finally, the product after the reaction was washed with ultrapure water until it was neutral, and dried in vacuum at 20°C and 0.02MPa for 2h. Prepared chrysanthemum-shaped lightweight magnetic nanospheres;

[0046] Step 2. Place 30mg of chrysanthemum-shaped lightweight magnetic nanospheres, 20mg of estradiol, 20mg of bovine serum albumin, 1.5g of tris(2-carbonylethyl) phosphate hydrochloride and 30mL of phosphate buffer into a 100mL three-necked flask at room temperature Rotate at 100r / min, stir for ...

Embodiment 3

[0057] A method for preparing a chrysanthemum-shaped lightweight protein molecularly imprinted magnetic nanomaterial, comprising the following steps:

[0058] Step 1. Put 1.5g of ferric chloride, 4.0g of anhydrous sodium acetate, 4.0g of PEG-400 and 40mL of ethylene glycol in a 100mL three-necked flask, and stir for 6 hours in an oil bath at 200°C at a speed of 150r / min. Then the reaction product was poured into a 50mL polytetrafluoroethylene reactor and reacted at 170°C for 6h. Finally, the product after the reaction was washed to neutral with ultrapure water, and vacuum-dried at 30°C and 0.03MPa for 3h. Prepared chrysanthemum-shaped lightweight magnetic nanospheres;

[0059] Step 2. Place 40mg of chrysanthemum-shaped lightweight magnetic nanospheres, 30mg of estradiol, 25mg of bovine serum albumin, 2.0g of tris(2-carbonylethyl) phosphate hydrochloride and 40mL of phosphate buffer into a 100mL three-necked flask at room temperature Rotate at 180r / min, stir for 4 hours, incub...

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Abstract

The invention discloses a chrysanthemum-shaped light-weight repellent protein molecularly imprinted magnetic nanomaterial and its preparation method and application. Firstly, PEG-modified magnetic nanospheres are synthesized by a solvothermal method; then, the PEG-modified magnetic nanospheres and Estradiol is added to the modified bovine serum albumin buffer solution to obtain a template-carrier complex; finally, the template molecule estradiol is removed with an eluent, and the solid polymer is separated by an external magnetic field, and the separated After the solid polymer is eluted and dried, the chrysanthemum-like lightweight magnetic nanomaterial that can exclude protein molecular imprinting is prepared. The chrysanthemum-shaped light-weight, exclusionable protein molecularly imprinted magnetic nanomaterial prepared by the invention has uniform particle size, good structural stability, strong magnetic response, good reusability, and strong selective adsorption capacity for estradiol , High adsorption capacity per unit mass, high recovery rate, good exclusion effect on protein in milk, low material cost and simple preparation.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation and food analysis application, and relates to a chrysanthemum-shaped lightweight protein molecularly imprinted magnetic nanomaterial and its preparation method and application. Background technique [0002] As a new type of pollutant, environmental estrogen interferes with the endocrine system and affects the physiological functions of hormones by antagonizing or simulating endocrine hormones. There are many types and wide distribution, mainly including bisphenols, steroids, agrochemicals and phthalates. Environmental estrogens, even at very low concentrations, can cause irreversible damage to sex and reproductive function in wild animals. What's more serious is that environmental estrogen can enter human or animal body through food chain or direct contact. It combines with estrogen receptors to cause endocrine dysfunction, gonadal hypogenesis, congenital malformations, and dysf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30B01D15/08
CPCB01J20/262B01J20/28009B01J20/28042B01D15/08
Inventor 张军杰王悦赵文昌白艳红郑元锁郑阿群李健军
Owner XI AN JIAOTONG UNIV