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Material pretreatment method for preparing tea theanine

A technology of tea theanine and pretreatment, which is applied in the field of separation and purification of theanine in tea leaves, can solve the problems of increasing the pressure of sewage treatment, increasing the production process steps, and taking a long time.

Active Publication Date: 2020-12-22
HUNAN JINNONG BIOLOGICAL RESOURCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the feed liquid obtained by ultrafiltration treatment has good clarity and can effectively retain macromolecular impurities, the actual operation process takes a long time. The decolorization of macroporous adsorption resin increases the production process steps, which directly leads to labor, equipment and material costs. increase, and the ultrafiltration membrane and resin cleaning process will also increase the pressure of sewage treatment

Method used

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  • Material pretreatment method for preparing tea theanine
  • Material pretreatment method for preparing tea theanine
  • Material pretreatment method for preparing tea theanine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Collect the waste liquid produced by the production of tea polyphenols in the workshop;

[0035] (2) Dilute the waste liquid with purified water to a concentration of 7.6Brix (use ATAGO PAL-1 portable sugar meter to detect the concentration of the diluted waste liquid, the same below);

[0036] (3) Then use the prepared NaOH solution (mass fraction 50%) to adjust the pH of the waste liquid (monitor the pH value of the waste liquid with a pH meter, the same below): add NaOH solution while stirring until the pH value reaches 10.0, and the alkali-adjusting material is obtained ;

[0037] (4) Take 500mL 001×7 resin and put it on the chromatography column. After cleaning, put the base adjustment material directly on the column; control the flow rate of the column at 450mL / h, and load a total of 2503g on the column. After the column is finished, use the same Washing at a flow rate of 750mL, the washing liquid is incorporated into the effluent of the upper column; the eff...

Embodiment 2

[0040] (1) Collect the waste liquid produced by the production of tea polyphenols in the workshop;

[0041] (2) Dilute the waste liquid to a concentration of 8.0 Brix with purified water;

[0042] (3) Then use the prepared KOH solution (mass fraction 45%) to adjust the pH: add KOH solution while stirring until the pH value reaches 9.7, and the alkali adjustment material is obtained;

[0043] (4) Take 500mL 001×7 resin and put it on the chromatography column. After cleaning, put the base adjustment material directly on the column; control the flow rate of the column at 400mL / h, and load a total of 2507mL on the column. After the column is finished, use the same Washing at a flow rate of 750mL, the washing liquid is incorporated into the effluent of the upper column; the effluent of the upper column and the washing liquid are mixed evenly, which is the material for the preparation of theanine.

Embodiment 3

[0045] (1) Collect the waste liquid produced by the production of tea polyphenols in the workshop;

[0046] (2) Dilute the waste liquid to a concentration of 7.8Brix with purified water;

[0047] (3) Then use the prepared NaOH solution (50% mass fraction) to adjust the pH of the solution: add NaOH solution while stirring until the pH value reaches 9.5, and the alkali adjustment material is obtained;

[0048] (4) Take 700mL of 001×7 resin and put it on the chromatography column. After cleaning, put the base adjustment material directly on the column; control the flow rate of the column at 680mL / h, a total of 3502mL is loaded on the column, and wash with water at the same flow rate after the column is loaded , the amount of water washing is 1050mL, and the water washing liquid is merged into the effluent of the upper column. The effluent from the upper column and the washing liquid are evenly mixed, which is the material for preparing theanine.

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Abstract

The invention provides a material pretreatment method for preparing tea theanine. The method comprises the following steps: diluting a tea polyphenol waste liquid collected in a workshop, adjusting the pH value of the material to be slightly alkaline, and then performing cation exchange resin column chromatography treatment. According to the invention, the method is simple to operate, low in treatment cost, low in equipment investment, high in efficiency and free of environmental pollution; the trouble of precipitation caused by material acid regulation can be effectively solved, so that the stability and smoothness of the subsequent theanine preparation column chromatography process are ensured; and the recovery rate of theanine in tea leaves reaches 94% or above, so that great significance is achieved in improving the quality of theanine products in tea leaves and increasing the development scale and depth of theanine-related products.

Description

technical field [0001] The invention relates to a method for separating and purifying theanine in tea, in particular to a method for removing impurities of materials before the preparation of tea theanine. Background technique [0002] In July 2014, tea theanine was approved as a new food raw material. It is the most abundant free amino acid in tea, accounting for more than 50% of the total free amino acids, and its content in tea is about 1% to 2%. It is a natural functional factor that lowers blood pressure, improves cognition, relieves anxiety and boosts immunity. [0003] At present, the production of theanine mainly includes in vitro enzymatic synthesis, extraction, purification and separation from tea leaves, microbial fermentation preparation and plant tissue cell culture. Among them, the extraction and purification from tea leaves is the most direct, and can better guarantee the original natural chemical properties and effective and safe functional properties of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C237/06C07C231/24
CPCC07C231/24C07C237/06
Inventor 孙建龙伟岸金慧罡殷梦龙饶昆范水源
Owner HUNAN JINNONG BIOLOGICAL RESOURCES
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