Method for rapidly identifying pathogenicity of tobacco fusarium

Abstract

The invention relates to the technical field of tobacco disease identification, in particular to a method for rapidly identifying pathogenicity of tobacco fusarium. The method comprises the followingsteps of 1, conducting heating and punching; 2, washing true leaves with sterile water; 3, adding sterile water for mixing; 4, covering with a cover; and 5, conducting observing and recording. The method has the advantages that a fusarium crude toxin preparation method is mature, culture medium materials for preparing fusarium crude toxin are cheap, and a preparation method is simple; a culture dish used in the method is made of transparent materials, so that symptoms of roots of tobacco plants can be observed easily; and a test method is simple, easy to operate and free of special training.

Description

technical field [0001] The invention relates to the technical field of tobacco disease identification, in particular to a rapid pathogenicity identification method of Fusarium tobacco. Background technique [0002] Fusarium genus incomplete fungal class, Polygonal order, Tumoracneceae, Fusarium genus, widely distributed in nature, some cause diseases of wheat, rice and vegetables; some are beneficial to industry and agriculture, such as gibberellin (920) from Produced by Fusarium moniliforme; some cause fungal diseases in humans and animals, such as Fusarium solani (F. solani) can cause corneal ulcers, sometimes invade the skin and nails, and even poisoning (fusarium toxin), in taxonomy, Fusarium The asexual period originally belonged to the Deuteromycota subphylum, and the sexual period belonged to the Ascomycota subphylum. According to the ninth edition of the "Dictionary of Fungi" in 2001, Fusarium belongs to the category of asexual fungi, and the sexual period is Ascomy...

AI Technical Summary

Problems solved by technology

[0003] Tobacco root rot caused by Fusarium is an important rhizome disease in tobacco leaf production. The typical symptoms of the disease are weak growth of diseased tobacco plants, gradual yellowing and wilting of tobacco leaves from bottom to top, and necrosis at the base of tobacco stems. Water-soaked brown necrosis appears on the taproot and main root. When the diseased stem and root are cut open, xylem browning can be observed. In severe cases, the tobacco plant will die. Fusarium as a saprophytic fungus attaches to the roots of tobacco plants. From the diseased plants brought back by scientific and technological workers from the tobacco fields, a large number of Fusarium isolates are non-pathogenic Fusarium saprophytes, which are pathogenic to a large number of strains. Screening of strong pathogenic strains is a necessary basic work. At present, commonly used pathogenicity determination methods such as spore suspension root soaking method, spore suspension root irrigation method, and grain grafting method are time-consuming, and environmental factors The impact is relatively large, and the repeatability is poor, which brings great troubles to the later in-depth research and scientific research. The current method for determining the pathogenicity of tobacco Fusarium has a long identification period and poor repeatability, and the root symptoms are not easy to observe.

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