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Method of Improving the Mechanical Properties of Microbial Cemented Sand and Soil Using Protein Cementitious Materials

A technology of microbial glue and mechanical properties, which is applied in the field of improving the mechanical properties of microbial cemented loose sand and soil by using protein gelling materials, and improving the mechanical properties of microbial cemented loose sand, which can solve the problem of biological activity and function of exogenous additives that do not have artificial directional control And other issues

Active Publication Date: 2021-08-20
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the difficulty of using exogenous additives in the prior art to greatly improve the strength and toughness of loose sandy soil cemented by microorganisms at the same time, and there is no technical defect or improvement requirement such as artificially directional regulation of the structure, biological activity and function of exogenous additives, the present invention provides A method of improving the mechanical properties of microbial cemented sand by using protein gelling materials

Method used

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  • Method of Improving the Mechanical Properties of Microbial Cemented Sand and Soil Using Protein Cementitious Materials
  • Method of Improving the Mechanical Properties of Microbial Cemented Sand and Soil Using Protein Cementitious Materials
  • Method of Improving the Mechanical Properties of Microbial Cemented Sand and Soil Using Protein Cementitious Materials

Examples

Experimental program
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Embodiment 1

[0065] Take a certain amount of quartz sand, add 1 times the pore volume water and mix well. Add it into the rectangular mold of 40×40×160mm in three times, and fill in one third of the sand and compact it to the third division line of the mold height. Keep the sandy soil saturated after compaction and filling. Prepare a mixed bacterial solution after activating the Bacillus sarcina bacteria solution, the formula is: 0.5M CaCl 2 Mix with bacteria solution according to 1:10 (V / V). Inject 0.4 times the pore volume of bacterial solution into the filled sand, and then pour 0.6 times the pore volume of the mixed bacterial solution at 30°C for 8 hours for bacterial immobilization. Use amino acid polymers such as polyglutamic acid (PGA) as protein gelling material to prepare fresh protein cementing solution, the formula is: 0.5M urea, CaCl 2 0.5M, PGA 2.25g / L. Pour 1 times the pore volume of fresh protein cementing solution into the sand treated with bacteria immobilization. Aft...

Embodiment 2

[0068] In this embodiment, the steps are basically the same as in Embodiment 1, the difference is that:

[0069]In the sand mixing and sample loading step, the rectangular mold of 40×40×160 mm in Example 1 was replaced with a cylindrical mold with a height of 60 mm and a diameter of 30 mm. Only perfuse the bacterial solution once before perfusing the protein cement solution, and no longer perfuse the bacterial solution during the perfusion of the protein cement solution. In the step of perfusing fresh protein cementing fluid, the polyglutamic acid (PGA) in Example 1 was replaced with a small molecule protein such as bovine serum albumin (BSA), and the fresh protein cementing fluid was prepared at a concentration of 2.25 g / L. A total of 14 fresh cementing fluids were perfused for a total of 7 days. The unconfined compressive test was carried out on the cemented sand, and the unconfined compressive strength of the sample was 1.61MPa, and the toughness was 2.40MJ / m 3 . The unc...

Embodiment 3

[0071] In this embodiment, the steps are basically the same as in Embodiment 1, the difference is that:

[0072] In the sand mixing and sample loading step, the rectangular mold of 40×40×160 mm in Example 1 was replaced with a cylindrical mold with a height of 60 mm and a diameter of 30 mm. Only perfuse the bacterial solution once before perfusing the protein cement solution, and no longer perfuse the bacterial solution during the perfusion of the protein cement solution. In the step of perfusing fresh protein cementing fluid, the PGA in Example 1 was replaced with natural protein hydrolyzate such as wool hydrolyzed keratin, and the fresh protein cementing fluid was prepared according to the concentration of 0.44g / L. A total of 14 fresh cementing fluids were perfused for a total of 7 days. The unconfined compressive test was carried out on the cemented sand, and the unconfined compressive strength of the sample was 1.88MPa, and the toughness was 2.50MJ / m 3 . The unconfined ...

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Abstract

The invention relates to a method for improving the mechanical properties of microbial cemented sandy soil by using protein gelling materials, and belongs to the interdisciplinary technical field of geotechnical (geological) engineering and microorganisms. The cementing liquid of the present invention contains protein gelling material, urea and calcium chloride. In the process of cementation and solidification, the carbonate radical produced by bacterial hydrolysis of urea and the calcium ion produced by ionization of calcium chloride form calcium carbonate precipitation, and the protein gelling material passes through The chemical bonds form an effective connection between the carbonate and the sand particles, so as to achieve the purpose of cementing the sand, and the protein cementitious material is evenly distributed between the sand particles, providing more nucleation sites for calcium carbonate precipitation point, which is beneficial to the precipitation of calcium carbonate. At the same time, the viscosity and chain-like structural characteristics of protein gelling materials improve the interface mechanics between sand particles and calcium carbonate, and improve the toughness of microbial cemented sand.

Description

technical field [0001] The invention belongs to the interdisciplinary field of geotechnical (geological) engineering and microorganisms, and more specifically relates to a method for improving the mechanical properties of microbially cemented loose sandy soil, in particular to a method for improving microbially cemented loose sandy soil mechanical properties by using protein cementitious materials. performance method. Background technique [0002] With the acceleration of urbanization, the demand for traditional cementing materials such as cement and lime has increased unprecedentedly worldwide. However, traditional cementing materials have disadvantages such as high energy consumption and large pollution. It is urgent to develop new types of cement that are efficient, environmentally friendly and economical Coagulation materials should be improved or partially replaced. [0003] Microbial-induced carbonate precipitation (MICP) is the most common microbial mineralization de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C04B26/20C04B26/02
CPCC04B26/026C04B26/20C04B2111/00732C04B2201/50C04B24/14C04B22/124C04B24/126C04B2103/0001C04B14/361
Inventor 闫云君姚敦璠吴姣袁升王磊阎金勇徐莉杨敏
Owner HUAZHONG UNIV OF SCI & TECH
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