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Preparation method for multiple-response type nanodrug based on combined treatment

A multi-response, nano-drug technology, applied in the field of biomedical materials, can solve the problems of poor penetration ability, obvious toxic and side effects, and poor therapeutic effect, and achieve the effects of low toxic and side effects, simplifying experimental steps, and improving biocompatibility

Pending Publication Date: 2020-12-29
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the shortcomings of the existing conventional cancer treatment methods, which have obvious toxic side effects and poor therapeutic effect, the present invention provides a new drug delivery system, which integrates chemotherapy, photothermal therapy and photodynamic therapy into one system to realize the drug delivery in time and space. Synergistic effect of controlled release and multiple therapeutic modalities
[0006] When the nanoparticles accumulate in the tumor site, the gelatin shell can be degraded by MMP-2, etc., realizing the size conversion of the nanoparticles, releasing GO with a smaller size to penetrate into the deep tumor effectively, and solving the penetration ability of general anti-tumor drugs bad question

Method used

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  • Preparation method for multiple-response type nanodrug based on combined treatment
  • Preparation method for multiple-response type nanodrug based on combined treatment
  • Preparation method for multiple-response type nanodrug based on combined treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: 2 mL of aqueous gelatin solution (25 mg / mL) was added to a sample bottle containing 1 mL of graphene oxide aqueous dispersion (1 mg / mL), the reaction temperature was 45 ° C, and the reaction was stirred at a speed of 1000 r / min for 2 h. After the reaction, drop it into 10 mL of dioctyl succinic sodium sulfonate in dichloromethane solution (25 mg / mL) and stir and emulsify at a speed of 1000 r / min for 10 min, and then drop the emulsion into 45 mL of polyvinyl alcohol (type 1788) Stir and emulsify in an aqueous solution (20mg / mL) at a speed of 1000r / min for 10min, and finally add 5mg of N,N'-bis(acryloyl)cystamine, the reaction temperature is 38°C, the stirring speed is 800r / min, and the reaction time is 12h. After the reaction, centrifuge with a high-speed centrifuge at a speed of 10000r / min for 20min, collect the precipitate and wash it with pure water for 3 times, and finally disperse it with 10mL of pure water to obtain the nano-delivery system of the present...

Embodiment 2

[0030] Example 2: Dissolve 0.5 mg of doxorubicin hydrochloride with 1 mL of depure water, then slowly drop it into a sample bottle containing 1 mL of graphene oxide aqueous dispersion (1 mg / mL), stir at a speed of 500 r / min, and the reaction temperature at 37°C, and the reaction time was 1 hour. After the reaction, use a high-speed centrifuge to centrifuge at a speed of 10000r / min for 20min, remove the supernatant, disperse the precipitate with pure water and centrifuge again (5mL×3), and finally disperse with 2mL pure water. Then, 1 mL of aqueous gelatin solution (25 mg / mL) was added to the dispersion, the reaction temperature was 45° C., and the reaction was stirred at a speed of 1000 r / min for 2 h. After the reaction, drop it into 10 mL of dioctyl succinic sodium sulfonate in dichloromethane solution (25 mg / mL) and stir and emulsify at a speed of 1000 r / min for 10 min, and then drop the emulsion into 45 mL of polyvinyl alcohol (type 1788) Stir and emulsify in an aqueous so...

Embodiment 3

[0031]Example 3: 0.5 mg of doxorubicin hydrochloride and 0.5 mg of indocyanine green were dissolved in 1 mL of depurified water, and then slowly dripped into a sample bottle containing 1 mL of graphene oxide aqueous dispersion (1 mg / mL) to Stir at a rotation speed of 500 r / min, the reaction temperature is 37° C., and the reaction time is 1 hour. After the reaction, use a high-speed centrifuge to centrifuge at a speed of 10000r / min for 20min, remove the supernatant, disperse the precipitate with pure water and centrifuge again (5mL×3), and finally disperse with 2mL pure water. Then, 1 mL of aqueous gelatin solution (25 mg / mL) was added to the dispersion, the reaction temperature was 45° C., and the reaction was stirred at a speed of 1000 r / min for 2 h. After the reaction, drop it into 10 mL of dioctyl succinic sodium sulfonate in dichloromethane solution (25 mg / mL) and stir and emulsify at a speed of 1000 r / min for 10 min, and then drop the emulsion into 45 mL of polyvinyl alco...

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Abstract

The invention provides a design and preparation method for a multiple-response type nanodrug based on combined treatment. The design and preparation method comprises the following steps of: through graphene oxide, loading adriamycin and indocyanine green, then, coating the graphene oxide with gelatin, obtaining nanometer particles with an even size through a secondary emulsification method, and finally, carrying out crosslinking through N, N'-bis (acrylyl) cystamine to obtain the multiple-response type nanodrug. The design and preparation method adopts the gelatin as a nanoparticle shell, biocompatibility is improved, in addition, the multiple-response type nanodrug has size conversion capability, a penetration effect of the nanodrug on a tumor position is improved, the combined treatmentcan be realized through the graphene oxide, the adriamycin and the indocyanine green, and an anti-tumor effect is obviously improved.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to the design and preparation method of an anti-tumor drug delivery system. Background technique [0002] Traditional cancer treatment methods include surgery, chemotherapy and radiation therapy. However, surgical resection has a high recurrence rate, and chemotherapy and radiotherapy have significant side effects on normal tissues. Therefore, drug delivery systems came into being, such as liposomes, micelles, and hydrogels. However, a single treatment method cannot achieve the desired effect. At this stage, it is mainly through integrating multiple treatment methods into one system to establish a combined treatment method to achieve better anti-tumor effects. [0003] Emerging cancer treatment methods include photothermal therapy (PTT), photodynamic therapy (PDT), sonodynamic therapy (SDT) and immunotherapy. Among them, there are more and more studies on PTT and P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/704A61K9/52A61K47/42A61K47/20A61K47/04A61P35/00
CPCA61K41/0052A61K41/0057A61K31/704A61K9/5169A61K9/5123A61K9/5115A61P35/00
Inventor 吴波震李明沛温兴翰
Owner ZHEJIANG UNIV OF TECH
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