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Application of Morinda officinalis extract as Vibrio parahaemolyticus inhibitor, Vibrio parahaemolyticus inhibitor and preparation method thereof

A technology of hemolytic Vibrio and Morinda citrifolia, which is applied to the inhibitor of Vibrio parahaemolyticus and its preparation, and the application field of Morinda citrifolia extract as the inhibitor of Vibrio parahaemolyticus, which can solve the damage of microbalance immunity , Vibrio drug resistance, complex preparation process and other problems

Active Publication Date: 2022-05-03
HAINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to avoid economic losses, the current aquaculture industry mainly uses antibiotics to prevent and control Vibrio parahaemolyticus, but this has obvious disadvantages, namely: the generation of Vibrio drug resistance, the residue of antibiotics, the microbalance of aquatic animals and The destruction of immunity, therefore, the search for an effective natural antibacterial substance has become the focus of attention
Related studies have shown that pepper seed and garlic extracts also have inhibitory effects on Vibrio parahaemolyticus, but the effective antibacterial substances are extracted by organic solvent absolute ethanol, and its preparation process is complicated. If it is put into aquaculture industry, the yield too low, too expensive

Method used

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  • Application of Morinda officinalis extract as Vibrio parahaemolyticus inhibitor, Vibrio parahaemolyticus inhibitor and preparation method thereof
  • Application of Morinda officinalis extract as Vibrio parahaemolyticus inhibitor, Vibrio parahaemolyticus inhibitor and preparation method thereof
  • Application of Morinda officinalis extract as Vibrio parahaemolyticus inhibitor, Vibrio parahaemolyticus inhibitor and preparation method thereof

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preparation example Construction

[0030] The present invention also provides a preparation method of Vibrio parahaemolyticus inhibitor, comprising the following steps:

[0031] a) After mixing Morinda officinalis with water and boiling, solid-liquid separation is carried out to obtain a separation liquid;

[0032] b) Concentrating the separated liquid to obtain the Vibrio parahaemolyticus inhibitor.

[0033] In the present invention, Morinda officinalis and water are mixed and boiled to extract, and then concentrated to obtain the extract, which can be used as an inhibitor of Vibrio parahaemolyticus to effectively inhibit Vibrio parahaemolyticus; and the preparation method of the present invention is simple and easy, and water extraction That is, there is no need to use organic solvents, which greatly reduces the cost.

[0034] Regarding step a): after mixing Morinda officinalis and water and boiling, solid-liquid separation is carried out to obtain a separation liquid.

[0035] In the present invention, the...

Embodiment 1

[0060] 1.1 Raw material and preparation

[0061] Raw materials: sun-dried Morinda officinalis

[0062] method:

[0063] S1. After crushing 30g of sun-dried Morinda officinalis fruit, add 300mL of tap water, soak for 30min, heat and boil for 5min (first boil for 1min, then boil for 4min, stir while cooking), and then filter with 4 layers of emery cloth , to obtain a filtrate and a filter residue.

[0064] S2. Add 100 mL of tap water to the primary filter residue, directly heat and boil for 5 minutes, and then filter with 4 layers of emery cloth to obtain the secondary filtrate and secondary filter residue.

[0065] S3. After combining the primary filtrate and the secondary filtrate, concentrate to 30 mL, and the concentration of the obtained extract at this time is 1 g / mL.

[0066] 1.2 Antibacterial test against Vibrio parahaemolyticus

[0067] (1) Preparation of bacteria solution

[0068] Take out the strain of Vibrio parahaemolyticus frozen in glycerol, inoculate it on a...

Embodiment 2

[0080] Determination of the effect of ultraviolet rays on the antibacterial ability of Morinda officinalis extract by filter paper method

[0081] Get 15 mL of the stock solution of Morinda officinalis fruit extract obtained in Example 1 and place it in a sterilized petri dish to irradiate with ultraviolet light. , Take 10 μL of Morinda officinalis extract stock solution and absorb it on a sterilized filter paper sheet, and place it on the surface of the bacteria-containing plate with sterile tweezers. The plate was placed in a biochemical incubator at 30°C for constant temperature incubation for 12-16 hours, and the diameter of the inhibition zone was observed and recorded. Three repetitions were set up for each test group, 3 pieces of filter paper were placed on each plate, and the diameter of the inhibition zone was averaged. The results are shown in Table 3 and figure 1 .

[0082] Table 3 Bacteriostatic effect of Morinda officinalis fruit extract treated with ultraviole...

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Abstract

The invention provides the application of Morinda citrifolia extract as an inhibitor of Vibrio parahaemolyticus, an inhibitor of Vibrio parahaemolyticus and a preparation method thereof. The preparation method of the vibrio parahaemolyticus inhibitor provided by the present invention comprises the following steps: a) after mixing Morinda citrifolia with water and boiling, the solid-liquid separation is carried out to obtain a separation liquid; b) the separation liquid is concentrated to obtain the parahaemolyticus Hemolytic Vibrio Inhibitor. In the present invention, Morinda citrifolia is mixed with water to be boiled and extracted, and then concentrated to obtain an extract, which can be used as an inhibitor of Vibrio parahaemolyticus and can effectively inhibit Vibrio parahaemolyticus; and the preparation method of the present invention is simple and feasible, and the water extraction That is, no organic solvent is required, which greatly reduces the cost.

Description

technical field [0001] The invention relates to the field of biology, in particular to the application of Morinda officinalis extract as an inhibitor of vibrio parahaemolyticus, the inhibitor of vibrio parahaemolyticus and a preparation method thereof. Background technique [0002] Litopenaeus vannamei, also known as Penaeus vannamei, has the characteristics of strong environmental adaptability, fast growth, strong disease resistance, and significant economic benefits. Yield is one of the species of shrimp farming. With the continuous improvement of the scale of shrimp farming, the frequency of shrimp farming diseases has also increased accordingly. The most common diseases are caused by Vibrio parahaemolyticus, such as red body disease, red leg disease, white spot disease, enteritis disease, gill rot disease, etc. Caused the prawn farming industry to suffer huge loss. Vibrio parahaemolyticus is a Gram-negative bacterium, and its pathogenic mechanism is complex, which is t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/746A61P31/04
CPCA61K36/746A61P31/04
Inventor 周海龙常逢彤黎娜裴跃斌路延笃刁晓平
Owner HAINAN UNIV
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