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A kind of culture medium for identification of Phellinus mulberry and its preparation method and application

An identification method and culture medium technology, which are applied in the field of medium production, can solve the problems of Phellinus japonica confusion, restriction of Phellinus japonica development, and difficulty in becoming stable Phellinus japonica, and achieve low cost, strong operability, and simple identification method Effect

Active Publication Date: 2021-08-31
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the confusion of understanding of Phellinus Phellinus in the market and academic circles has seriously restricted the development of Phellinus Huang, an ancient and miraculous traditional Chinese medicine, making it difficult for Phellinus Huang to become a stable traditional Chinese medicine.

Method used

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  • A kind of culture medium for identification of Phellinus mulberry and its preparation method and application
  • A kind of culture medium for identification of Phellinus mulberry and its preparation method and application
  • A kind of culture medium for identification of Phellinus mulberry and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, Morus Phellinus Phellinus and other strains are isolated and cultivated

[0050] (1) Retrieve the yellow sessile fungi that are often mistaken for Phellinus Phellinus in the literature and the Internet (Table 1); the fruiting bodies of Phellinus Phellinus and various fruiting bodies that are easily confused with Phellinus Phellinus ( figure 1 ).

[0051] Table 1: Phellinus mulberry and various fruiting bodies that are easily confused with Phellinus mulberry

[0052]

[0053]

[0054] (2) Isolate the above-mentioned various sporocarp strains and temporarily store them in PDA medium. Above-mentioned strains all can grow smoothly in PDA culture medium, only from aspects such as color, shape, smell, can't accurately distinguish which one is the true mulberry Phellinus ( figure 2 A).

Embodiment 2

[0055] Embodiment 2, mulberry Phellinus identification medium making

[0056] (1) Take the fresh silkworm excrement from the day before the 5th instar silkworm, and the silkworm is required to be healthy and disease-free, and the silkworm excrement is pure (only a small amount of broken mulberry leaves eaten by silkworms, without any anti-stiffness powder and lime and other sericulture medicines) );

[0057] (2) Get a 5-mesh sieve, initially sieve fresh silkworm excrement, and remove larger broken mulberry leaves;

[0058] (3) Dry at 60°C for 10 hours, select by air once with an electric fan, and manually select again with tweezers to remove smaller mulberry leaf debris;

[0059] (4) Place in a 150-mesh sieve, wash once with clear water, and further remove the fine mulberry leaf debris attached to the surface of the silkworm excrement to obtain pure silkworm excrement before the 5th instar (without any other impurities except the silkworm excrement).

[0060] (5) Dry again a...

Embodiment 3

[0063] Embodiment 3, mulberry Phellinus identification

[0064] (1) Pick the above-mentioned various bacterial strains grown in the PDA medium, transfer them to the above-mentioned mulberry Phellinus identification medium, and seal with a parafilm.

[0065] (2) Culture in complete darkness at 28°C for 10-12 days.

[0066] (3) Under the above-mentioned culture conditions, only the mycelium of Phellinus mulberry can grow normally in the above-mentioned medium, while the tree species parasitic by other strains tested are not mulberry, and they cannot grow normally in the identification medium of Phellinus mulberry ( figure 2 B).

[0067] (4) the Phellinus mulberry strain is cultivated with the above-mentioned identification medium for a long time, and there is no phenomenon such as any degradation and variation of the Phellinus mulberry strain in 5 years ( image 3 ), using pure mulberry branches to make fruiting body fungus bags (without any added substances), the fruiting bo...

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Abstract

The invention discloses a culture medium for identifying Phellinus mulberry, a configuration method and application thereof, and belongs to the field of biotechnology. The medium for identifying Phellinus mulberry of the present invention comprises the following components: silkworm excrement powder, KH 2 PO 4 , MgSO 4 1. Water, the mass ratio of which is 41.25:0.3:0.15:58.3, the pH value of the culture medium is 8.5-8.8, and then sterilized at 121°C for 30 minutes to make a culture medium for identification of Phellinus mulberry. Only the real mycelium of Phellinus mulberry can grow normally in the identification medium of Phellinus mulberry, while other strains cannot grow normally in the identification medium of Phellinus mulberry. The Phellinus mulberry strain was cultured and preserved in the above-mentioned identification medium for a long time, and no degradation, variation, etc. of the Phellinus mulberry strain was found within 5 years, and the Phellinus mulberry fruiting bodies could be successfully induced without rejuvenation. It proves that the culture medium can not only identify the real Phellinus mulberry, but also is completely suitable for the long-term preservation of the fungus Phellinus mulberry.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of a culture medium for identifying Phellinus mulberry. Background technique [0002] Sanghuangporus sanghuang is a very rare medicinal fungus that parasitizes on the mulberry tree (Morus L.). Phellinus is one of the medicinal fungi with the best anti-cancer efficiency recognized internationally [1] , Known as "Forest Gold". [0003] In the medical books of all dynasties since the Han Dynasty in my country, there are records of the use of Sanghuang. Such as "Shen Nong's Materia Medica" in the Han Dynasty, Tao Hongjing's "Bielu of Famous Doctors" in the Wei and Jin Dynasties, Zhen Quan's "On the Properties of Medicine" in the Early Tang Dynasty, etc. [2] . Although restricted by technical conditions, the records of Sanghuang by the ancients are often rough, and the descriptions of Sanghuang's name, texture, and usage are also different. It is even more difficu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02C12R1/645
CPCC12N1/02C12N1/14
Inventor 方荣俊方星辰
Owner JIANGSU UNIV OF SCI & TECH
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