Production process for jointly separating high-purity liquiritin, bitterness-removed glycyrrhizin and glycyrrhiza uralensis fisch total flavonoids from mono-ammonium glycyrrhizinate mother liquor paste

Abstract

The invention discloses a production process for jointly separating high-purity liquiritin, bitterness-removed glycyrrhizin and glycyrrhiza uralensis fisch total flavonoids from mono-ammonium glycyrrhizinate mother liquor paste. The production process comprises the following steps that (1) the mono-ammonium glycyrrhizinate mother liquor paste is taken, water is added to adjust the pH value, the mono-ammonium glycyrrhizinate mother liquor paste is dissolved and passes through a resin column 1# to be subjected to chromatography, water elution is carried out to obtain eluent for later use, a resin column 2# is subjected to elution through gradient dilute ethanol, and after the eluent is concentrated and dried under reduced pressure, a liquiritin crude product is obtained; ethanol with the concentration of 95% is added into the crude product, and recrystallization, filtration and drying are carried out to obtain the high-purity liquiritin; (2) the eluent for later use is taken, flows through a resin column 3# and is subjected to water elution, and the eluent is concentrated and dried to obtain the bitterness-removed glycyrrhizin; and (3) the resin columns 1#, 2# and 3# are regeneratedby using the ethanol with the concentration of 95%, eluent is collected and combined, and concentration and drying are carried out to obtain the glycyrrhiza uralensis fisch total flavonoids. Accordingto the production process for jointly separating the high-purity liquiritin, the bitterness-removed glycyrrhizin and the glycyrrhiza uralensis fisch total flavonoids from the mono-ammonium glycyrrhizinate mother liquor paste, the mother liquor paste after production of mono-ammonium glycyrrhizinate is adopted as a raw material, and the three products are separated and purified in a combined mode,so that the comprehensive utilization degree of the raw material is improved, the product value is high, cost is low, reproducibility is good, and the production process is suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to a production process for jointly separating high-purity liquiritin, glycyrrhizic acid and total flavonoids from glycyrrhizic acid monoammonium salt mother liquor paste, belonging to the field of food and medicine. Background technique [0002] Licorice (Glycyrrhiza uralensis Fisch) is a leguminous plant of the genus Glycyrrhiza licorice. There are 29 species and 6 varieties in the world. Among them, there are 3 varieties in 18 in my country, which are widely distributed in Northwest my country, North China, Northeast China and other regions. And Glycyrrhiza glabra is one of the basic plants of the medicinal material Glycyrrhiza glabra, which is widely used in medicine, food, cosmetics and other industries. [0003] The main active ingredients of licorice extract include glycyrrhizic acid and its various analogues of glycyrrhizin saponins, licorice total flavonoids of licorice flavonoids, such as liquiritin, isoliquiritigenin, gl...

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Problems solved by technology

The same production costs are high and there is no market competitiveness

[0010] Dalian Institute of Physical Chemistry, Chinese Academy of Sciences has applied for a patent named "A Preparation Method of Liquiritin" (application number: 200710011041.0). Porous resin, high-efficiency industrial chromatographic column separation, and freeze-drying to obtain liquiritin. This process is complex, requires high equipment, difficul