Ionic liquid and method for extracting codfish roe protein by utilizing same

A technology of ionic liquid and cod roe, which is applied in separation methods, peptide preparation methods, solid solvent extraction, etc., can solve the problems of protein variability and inactivation, poor protein stability, and low extraction efficiency, achieving efficient extraction and easy extraction. The effect of storage, good extraction effect

Active Publication Date: 2021-01-15
珠海中科先进技术研究院有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current method of extracting fish roe protein is mainly through water solvent extraction, but there are many problems in this method, such as low extraction efficiency, poor stability of extracted protein, protein variability and inactivation, etc.
Moreover, for differen

Method used

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  • Ionic liquid and method for extracting codfish roe protein by utilizing same
  • Ionic liquid and method for extracting codfish roe protein by utilizing same
  • Ionic liquid and method for extracting codfish roe protein by utilizing same

Examples

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Example Embodiment

[0032]Example 1: Preparation of ionic liquid and method for extracting cod roe protein by using it

[0033]An ionic liquid is mainly prepared from the following raw material components: L-carnitine and tartaric acid are stirred and rotated at a molar ratio of 1:1.

[0034]The method for extracting cod roe protein using the above ionic liquid includes the following steps:

[0035]Mix 10g of cod fish roe with 30g of the above-mentioned ionic liquid to pre-freeze and thaw. The pre-freezing temperature is -20°C and the thawing temperature is 25°C. The pre-freezing and thawing process is repeated 6 times. The thawed mixture Break the wall, stir at 6000r / min for 15min, and then centrifuge at 10000r / min for 15min, take the supernatant and filter, and take the filtrate. The resulting filtrate contains cod roe protein. Store at 20°C to 4°C.

Example Embodiment

[0036]Example 2: Preparation of ionic liquid and method for extracting cod roe protein by using it

[0037]An ionic liquid is mainly prepared from the following raw material components: matrine and salicylic acid are stirred and rotated at a molar ratio of 5:1.

[0038]The method for extracting cod roe protein by using the above ionic liquid includes the following steps:

[0039]Mix 15g of cod fish roe with 60g of the above ionic liquid, pre-freeze and thaw, the pre-freezing temperature is -80℃, the thawing temperature is 25℃, the pre-freezing and thawing process is repeated 8 times, the thawed mixture Break the wall, stir at 5000r / min for 10min, then centrifuge at 12000r / min for 20min, take the supernatant and filter, take the filtrate, and the resulting filtrate contains cod roe protein. Store at 20°C to 4°C.

Example Embodiment

[0040]Example 3: Preparation of ionic liquid and method for extracting cod roe protein by using it

[0041]An ionic liquid is prepared mainly from the following raw material components: theophylline and succinic acid are stirred and rotationally mixed in a molar ratio of 10:1.

[0042]The method for extracting cod roe protein by using the above ionic liquid includes the following steps:

[0043]Mix 30g of cod fish roe with 130g of the above-mentioned ionic liquid aqueous solution (the mass concentration of the ionic liquid in the ionic liquid aqueous solution is 70%), and pre-freeze and thaw. The pre-freezing temperature is -15°C and the thawing temperature is 25°C , The process of pre-freezing and thawing is repeated 12 times, the thawed mixture is broken, stirred at a speed of 6000r / min for 40min, and then centrifuged at a speed of 10000r / min for 20min, and the supernatant is filtered. Just take the filtrate, and the obtained filtrate contains cod roe protein and is stored at -20°C to 4°C....

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Abstract

The invention discloses an ionic liquid and a method for extracting codfish roe protein by utilizing the same. The ionic liquid is mainly prepared from the following raw material components: alkaloidand organic acid; the organic acid is selected from at least one of tartaric acid, salicylic acid, succinic acid and benzoic acid; and the molar ratio of the alkaloid to the organic acid is (1-12):(0.2-3). The method for extracting the codfish roe protein by utilizing the ionic liquid comprises the following steps: mixing codfish roes with an aqueous solution of the ionic liquid, performing pre-freezing and unfreezing, performing wall breaking, stirring and centrifuging on the unfrozen mixture, and filtering a supernate. The ionic liquid has a very good extraction effect on codfish roe proteinand high extraction efficiency, and the extracted protein has high biological activity and high stability and is easy to store.

Description

technical field [0001] The invention relates to an ionic liquid, in particular to a method for using the ionic liquid to extract animal protein, in particular to an ionic liquid and a method for using it to extract cod roe protein. Background technique [0002] Animal protein has high nutritional value, and fish protein is a very important animal protein. In China, cod is mainly produced in the Bohai Sea, the Yellow Sea and the northern part of the East China Sea. It is one of the marine economic fish species produced in the northern coast. The cod meat is delicious and nutritious, and can promote blood circulation and eliminate addiction; the swim bladder can replenish blood and stop bleeding; cod bones can cure athlete's foot; Cod pancreas contains a large amount of insulin; cod liver can be used to extract cod liver oil, and sturgeon roe is of high value, but it is not fully utilized. It is often simply eaten, resulting in insufficient utilization of sturgeon roe, and eve...

Claims

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Application Information

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IPC IPC(8): C07K1/14B01D11/02
CPCC07K1/145B01D11/0288Y02P20/54
Inventor 张嘉恒张基亮费玉清程艳芳李志超于明远黎穗芝黎钊明陈正件
Owner 珠海中科先进技术研究院有限公司
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