Application of Saccharomyces cerevisiae extract in the preparation of skin care products for the repair of human hsf and hacat cells damaged by uvb

Abstract

The invention discloses the application of brewer's yeast extract to the repair of human HSF and Hacat cells damaged by UVB, including the following steps: 1) the establishment of human skin cell HSF and Hacat ultraviolet UVB damage models: a) human normal skin cells, namely The culture of HSF cells and human immortalized epidermal keratinocytes, namely Hacat cells; b) UV radiation damages HSF cells and Hacat cells: use a small dose, namely 5mJ / cm 2 , multiple irradiation, total UVB dose 40mJ / cm 2 c) detection: observe the cell morphology under a microscope, and determine the relative survival rate of the cells; 2) continue to culture the injured HSF cells and Hacat cells with DMEM containing 1% FBS, and add beer After 24 hours of action of the yeast extract, the relevant skin vitality indicators SOD and MDA were measured. Saccharomyces cerevisiae extract has obvious effects on skin care and skin repair, and is basically non-toxic. It has the characteristics of high stability and storage resistance, and can be applied to the development of sunscreen and sunburn repair skin care products.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to the application of brewer's yeast extract in the preparation of skin care products for repairing human HSF and Hacat cells damaged by UVB. Background technique [0002] Ultraviolet rays are the general term for radiation with a wavelength of 100-400nm in the electromagnetic spectrum. According to the wavelength, ultraviolet rays are divided into long-wave UVA (315-400nm), medium-wave UVB (280-315nm), and short-wave UVC (100-280nm). Compared with UVA, UVB has much higher biological activity, so UVB is the main cause of photoaging damage. About 5% of UVB radiation can stay on the skin surface, and about 20% of it can penetrate the skin surface to reach the epidermis. The skin provides the basic function of an effective barrier against various environmental pressures. Therefore, it is particularly important to prevent skin damage caused by ultraviolet radiation. At prese...

AI Technical Summary

Problems solved by technology

The skin provides the basic function of an effective barrier against various environmental pressures. Therefore, it is particularly important to prevent skin damage caused by ultraviolet radiation. At present, sunscreens on the market have this effect, but they can only protect the skin from being damaged by ultraviolet rays. The effect of rapid skin recovery is not obvious

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