Efficient preparation method of active ingredients of honeysuckle leaves and antiviral application of active ingredients

A technology of active ingredient and honeysuckle leaves, applied in the field of separation of honeysuckle leaf extracts, which can solve problems such as waste of resources

Active Publication Date: 2021-01-26
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, as a by-product of honeysuckle harvesting, honeysuckle leaves are mostly rega...

Method used

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  • Efficient preparation method of active ingredients of honeysuckle leaves and antiviral application of active ingredients
  • Efficient preparation method of active ingredients of honeysuckle leaves and antiviral application of active ingredients
  • Efficient preparation method of active ingredients of honeysuckle leaves and antiviral application of active ingredients

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1. Preparation of Crude Samples

[0067] Pick the purchased dry honeysuckle leaves and discard the mixed honeysuckle and honeysuckle vines. Weigh 1 kg of selected dried honeysuckle leaves, put them into a round bottom flask after crushing, add 10 L of 95% ethanol according to the ratio of solid to liquid of 1:10, heat and reflux for extraction three times, each time for 2 hours. The ethanol crude extract of honeysuckle leaves obtained by extraction was suction-filtered and concentrated under reduced pressure to obtain 386 g of brown viscous ethanol extract.

[0068] The ethanol extract was made into a suspension with distilled water, and then distributed and extracted three times with equal volumes of petroleum ether, ethyl acetate, and n-butanol, concentrated under reduced pressure, and freeze-dried to obtain honeysuckle leaf petroleum ether extract, honeysuckle leaf ethyl acetate Ester extract, honeysuckle leaf n-butanol extract 22g, 19g, 135g, the extraction rate is...

Embodiment 2

[0115] 1.1 Concentration gradient preparation of the test sample solution

[0116] The various parts obtained by separating the ethyl acetate extract and n-butanol extract of honeysuckle leaves from LGCCC, ethanol extract of honeysuckle leaves, petroleum ether extract of honeysuckle leaves, ethyl acetate extract of honeysuckle leaves, n-butanol extract of honeysuckle leaves, honeysuckle leaves The remaining water-soluble matter was dissolved in 50% ethanol aqueous solution to prepare a 1 mg / mL test sample solution, and the 1 mg / mL test sample solution was serially diluted 2 times with 50% ethanol aqueous solution for a total of 9 times, and a total of 10 different samples were obtained. Concentrations and adjacent concentrations are 2-fold diluted test sample solutions.

[0117] Zanamivir was selected as the positive control group of the experiment, and chlorogenic acid was selected as the positive control group of natural components of the experiment, and a 0.005 mg / mL ...

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Abstract

The invention belongs to the technical field of honeysuckle extracts, and particularly relates to an efficient preparation method of active ingredients of honeysuckle leaves and antiviral applicationof the active ingredients. Honeysuckle is a bulk drug commonly used in China and is widely applied to the aspects of drugs, food and health care products, and due to the fact that the demand for honeysuckle is large and the supply-demand relationship is tense, related development of the honeysuckle leaves is carried out in the field to replace the honeysuckle so as to relieve the current situationof insufficient market supply. According to the method, the honeysuckle leaf extracts are used as research objects, the part with high antiviral activity is selected for research, a method for separating the honeysuckle leaf extracts based on linear elution countercurrent chromatography is provided, the ingredients with different polarities in the extracts can be separated to the maximum extent,and a neuraminidase inhibition experiment verifies that the extracts obtained through separation by means of the method have the ingredients with good antiviral activity.

Description

technical field [0001] The disclosure belongs to the technical field of countercurrent chromatography for separating natural extracts, and in particular relates to a method for separating honeysuckle leaf extracts through gradient elution countercurrent chromatography, the obtained extract and its antiviral application. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] Honeysuckle (Lonicera japonica Thunb.) is a perennial evergreen winding vine plant of the family Caprifoliaceae (Lonicera). It is an important traditional Chinese medicine resource in my country. It has various medicinal effects such as heat-clearing, anti-inflammatory, antibacterial, anti-viral . Honeysuc...

Claims

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Application Information

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IPC IPC(8): A61K36/355A61P31/16A61K127/00
CPCA61K36/355A61P31/16A61K2236/333A61K2236/55
Inventor 王岱杰崔莉林树乾赵恒强施树云郭莹宋祥云朱姮
Owner SHANDONG ANALYSIS & TEST CENT
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