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A kind of high-efficiency preparation method of honeysuckle leaf active ingredient and its antiviral application

A technology of honeysuckle leaf and antiviral activity, which is applied in the field of separation of honeysuckle leaf extract, can solve problems such as waste of resources, and achieve a significant inhibitory effect

Active Publication Date: 2022-03-18
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, as a by-product of honeysuckle harvesting, honeysuckle leaves are mostly regarded as having no use value and are directly discarded, resulting in a great waste of resources

Method used

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  • A kind of high-efficiency preparation method of honeysuckle leaf active ingredient and its antiviral application
  • A kind of high-efficiency preparation method of honeysuckle leaf active ingredient and its antiviral application
  • A kind of high-efficiency preparation method of honeysuckle leaf active ingredient and its antiviral application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1. Preparation of Crude Samples

[0067] Pick the purchased dry honeysuckle leaves and discard the mixed honeysuckle and honeysuckle vines. Weigh 1 kg of selected dried honeysuckle leaves, put them into a round bottom flask after crushing, add 10 L of 95% ethanol according to the ratio of solid to liquid of 1:10, heat and reflux for extraction three times, each time for 2 hours. The ethanol crude extract of honeysuckle leaves obtained by extraction was suction-filtered and concentrated under reduced pressure to obtain 386 g of brown viscous ethanol extract.

[0068] The ethanol extract was made into a suspension with distilled water, and then distributed and extracted three times with equal volumes of petroleum ether, ethyl acetate, and n-butanol, concentrated under reduced pressure, and freeze-dried to obtain honeysuckle leaf petroleum ether extract, honeysuckle leaf ethyl acetate Ester extract, honeysuckle leaf n-butanol extract 22g, 19g, 135g, the extraction rate is...

Embodiment 2

[0115] 1.1 Concentration gradient preparation of the test sample solution

[0116] The various parts obtained by separating the ethyl acetate extract and n-butanol extract of honeysuckle leaves from LGCCC, ethanol extract of honeysuckle leaves, petroleum ether extract of honeysuckle leaves, ethyl acetate extract of honeysuckle leaves, n-butanol extract of honeysuckle leaves, honeysuckle leaves The remaining water-soluble matter was dissolved in 50% ethanol aqueous solution to prepare a 1 mg / mL test sample solution, and the 1 mg / mL test sample solution was continuously diluted 2 times with 50% ethanol aqueous solution for a total of 9 times, and a total of 10 different samples were obtained. Concentrations and adjacent concentrations are 2-fold diluted test sample solutions.

[0117] Zanamivir was selected as the positive control group of the experiment, and chlorogenic acid was selected as the positive control group of natural components of the experiment, and a 0.005 mg / mL za...

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Abstract

The disclosure belongs to the technical field of honeysuckle extracts, and in particular relates to a high-efficiency preparation method of active ingredients of honeysuckle leaves and its antiviral application. Honeysuckle, as a commonly used bulk medicinal material in my country, has a wide range of applications in medicine, food, and health products. Due to the large demand for honeysuckle and the tense relationship between supply and demand, the development of honeysuckle leaves in this field is used as a substitute for honeysuckle to ease market supply. Insufficient status quo. This disclosure takes honeysuckle leaf extract as the research object, selects the part with higher antiviral activity for research, and provides a method for separating honeysuckle leaf extract based on linear elution countercurrent chromatography, which can separate the different The polar components are separated to the greatest extent, and the components with good antiviral activity in the extract separated by the above method are verified by the neuraminidase inhibition experiment.

Description

technical field [0001] The disclosure belongs to the technical field of countercurrent chromatography for separating natural extracts, and in particular relates to a method for separating honeysuckle leaf extracts through gradient elution countercurrent chromatography, the obtained extract and its antiviral application. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] Honeysuckle (Lonicera japonica Thunb.) is a perennial evergreen twining vine plant of the genus Lonicera in the family Caprifoliaceae. It is an important traditional Chinese medicine resource in my country. . Honeysuckle (also known as honeysuckle, silver flower, etc.) is the dry flower bud or the first fl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/355A61P31/16A61K127/00
CPCA61K36/355A61P31/16A61K2236/333A61K2236/55
Inventor 王岱杰崔莉林树乾赵恒强施树云郭莹宋祥云朱姮
Owner SHANDONG ANALYSIS & TEST CENT
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