Multifunctional injectable hydrogel and preparation method thereof

A multi-functional, water-injection technology, applied in the field of biomaterials and biomedical engineering, can solve the problems of susceptibility to infection, and achieve the effects of fast gelation, simple preparation process, and good biocompatibility

Pending Publication Date: 2021-01-29
NANKAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of wound diversity, susceptibility to infection and vascular lesions in diabetic foot ulcer lesion area, the present invention prepares cerium-loaded functionalized silicon-based bioactive glass injectable GelMA hydrogel, which is cross-linked under ultraviolet light to form a hydrogel , to fill the wound surface and slowly release cerium ions and silicon ions to exert antibacterial and angiogenesis functions, and promote wound healing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Synthesis of GelMA: Weigh 5g of gelatin and add it to 50mL of PBS solution, stir in a constant temperature water bath at 60°C until completely dissolved to make a gelatin solution with a concentration of 10% w / v, drop 3mL of MA at a rate of 0.5mL / min Add to the gelatin solution, and continue to react at 50°C for 3h; add 250mL PBS solution to the above reaction solution, stir for 30min and then terminate the reaction; place the reacted solution in a 12-14kDa dialysis bag, and deionize at 40°C Dialyze in water for 6 days, centrifuge at 10,000rpm for 10min, collect the supernatant, freeze-dry after pre-freezing, and obtain a white solid;

[0016] 2) Preparation of Ce-BG NPs: Mix 80 mL of absolute ethanol and 20 mL of deionized water, and add 4 g of DDA to the solution at a constant temperature of 40 °C, and stir for 10 min to form a uniform and transparent mixed solution; Add 15.9mL of TEOS at a rate of 0.5mL / min and stir for 30min to form a milky white solution; in the...

Embodiment 2

[0019] 1) Synthesis of GelMA: Weigh 5g of gelatin and add it to 50mL of PBS solution, stir it in a constant temperature water bath at 60°C until it is completely dissolved to make a gelatin solution with a concentration of 10% w / v, drop 4mL of MA at a rate of 0.5mL / min Add to the gelatin solution, and continue to react at 50°C for 3h; add 250mL PBS solution to the above reaction solution, stir for 30min and then terminate the reaction; place the reacted solution in a 12-14kDa dialysis bag, and deionize at 40°C Dialyze in water for 6 days, centrifuge at 10,000rpm for 10min, collect the supernatant, freeze-dry after pre-freezing, and obtain a white solid;

[0020] 2) Preparation of Ce-BG NPs: Mix 80 mL of absolute ethanol and 20 mL of deionized water, and add 4 g of DDA to the solution at a constant temperature of 40 °C, and stir for 10 min to form a uniform and transparent mixed solution; Add 15.9mL of TEOS at a rate of 0.5mL / min and stir for 30min to form a milky white solutio...

Embodiment 3

[0023] 1) Synthesis of GelMA: Weigh 5g gelatin and add it to 50mL PBS solution, stir it in a constant temperature water bath at 60°C until it is completely dissolved to make a gelatin solution with a concentration of 10% w / v, drop 5mL MA at a rate of 0.5mL / min Add to the gelatin solution, and continue to react at 50°C for 3h; add 250mL PBS solution to the above reaction solution, stir for 30min and then terminate the reaction; place the reacted solution in a 12-14kDa dialysis bag, and deionize at 40°C Dialyze in water for 6 days, centrifuge at 10,000rpm for 10min, collect the supernatant, freeze-dry after pre-freezing, and obtain a white solid;

[0024]2) Preparation of Ce-BG NPs: Mix 80 mL of absolute ethanol and 20 mL of deionized water, and add 4 g of DDA to the solution at a constant temperature of 40 °C, and stir for 10 min to form a uniform and transparent mixed solution; Add 15.9mL of TEOS at a rate of 0.5mL / min and stir for 30min to form a milky white solution; in the ...

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Abstract

The invention discloses multifunctional injectable hydrogel and a preparation method thereof, the prepared cerium-loaded functionalized silica-based bioactive glass injectable GelMA can be crosslinkedunder ultraviolet light to form hydrogel, uneven irregular wound surfaces can be filled with the hydrogel, cerium ions and silicon ions are slowly released, antibacterial and vascularization-promoting functions are exerted, and wound healing is promoted. The multifunctional injectable hydrogel disclosed by the invention is formed by physically mixing the prepared cerium functionalized silicon-based BGs and GelMA obtained by chemical grafting and then crosslinking under the irradiation of a photoinitiator and ultraviolet light. The hydrogel can be formed through in-situ injection, can be usedfor diabetic foot wounds, and can be well integrated with surrounding tissues; the silicon-based BGs in the hydrogel can play a role in resisting bacteria and promoting vascularization by releasing cerium ions and silicon ions, so that the infection of diabetic ulcer wounds can be prevented, and the problem of insufficient vascularization of the wounds can be solved.

Description

technical field [0001] The invention relates to a multifunctional injectable hydrogel biomaterial, specifically a cerium-loaded functionalized silicon-based bioactive glass injectable hydrogel and a preparation method thereof. It belongs to the field of biomaterials and biomedical engineering. Background technique [0002] Diabetic foot is one of the common chronic complications of diabetes. According to statistics, the probability of diabetes patients developing diabetic foot in their lifetime is 15% to 25%. The 5-year risk of death in patients with diabetic foot ulcers was 2.5 times that of diabetic patients without foot ulcers. The wounds formed by diabetic foot ulcers are characterized by various sizes and shapes, and the lesion area is prone to infection, accompanied by severe inflammatory response and vascular disease, which brings great challenges to the treatment. Therefore, tissue engineering materials with a single function cannot meet the treatment needs, and i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/52A61L27/10A61L27/22A61L27/54A61L27/50A61L27/02C08J3/075C08J3/24C08L89/00C08K9/02C08K3/40
CPCA61L27/025A61L27/10A61L27/222A61L27/50A61L27/52A61L27/54A61L27/60A61L2300/102A61L2300/404A61L2300/412A61L2400/06C08J3/075C08J3/24C08J2389/00C08K3/40C08K9/02
Inventor 王淑芳陈跃华饶洲峰董云生齐春晓王泊远王泽奇徐兰举
Owner NANKAI UNIV
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