Method for rapidly detecting glacial acetic acid in rice vinegar

Abstract

The invention relates to a method for rapidly detecting glacial acetic acid in rice vinegar, and the method is characterized in that the following steps: determining the content of HAc in pretreated rice vinegar by adopting an ultrahigh liquid chromatography-tandem mass spectrometry technology, quantifying by utilizing an isotope internal standard method, and establishing a calibration curve by taking the concentration ratio of a standard substance to an internal standard substance as an X axis and the peak area ratio of the standard substance to the internal standard substance as a Y axis; and calculating the content of glacial acetic acid in the rice vinegar. According to the invention, a rice vinegar sample is mixed with an isotope internal standard solution of acetic acid, a target object to be detected is separated from interference components in a rice vinegar matrix by using ultra-high performance liquid chromatography, the mass-to-charge ratio (m / z) of the target object to thecorresponding isotope internal standard is detected by using mass spectrometry, quantifying is performed by using an isotope internal standard method, and the content of acetic acid is calculated accurately. The method has the advantages of simple pretreatment, high sensitivity and strong characteristics, and can be used as a guidance method for acetic acid content determination.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for rapidly detecting glacial acetic acid in rice vinegar. Background technique [0002] Vinegar has antibacterial, anti-inflammatory, anti-fatigue, anti-oxidation, anti-aging, beauty, anti-cancer, and anti-cancer effects, and is used in daily life and medicine. Fruit vinegar is rich in nutrients and contains essential macroelements, trace elements and organic matter, etc., which can maintain physiological balance and prevent diseases. At present, the daily intake of vinegar cannot reach the per capita level, and the development of vinegar products has broad prospects; [0003] With the promulgation of vinegar standards, whether the quality of food additive acetic acid is qualified or not is directly related to the health of the people; the national standard GB 1886.10-2015 stipulates that it should meet the requirements of food additive acetic acid, but now there...

AI Technical Summary

Problems solved by technology

At present, the daily intake of vinegar cannot reach the per capita level, and the development of vinegar products has broad prospects;

[0003] With the promulgation of vinegar standards, whether the quality of food additive acetic acid is qualified or not is directly related to the health of the people; the national standard GB 1886.10-2015 stipulates that it should meet the requirements of food additive acetic acid, but now there will be industrial Acetic acid pretends to be a food additive, glacial acetic acid, and in order to adjust the acidity, hydrochloric acid, phosphoric acid, etc. are added, which is harmful to human health, and often contains some harmful substances

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