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Optimized preparation method of solid platelet-rich fibrin film

A platelet-rich and fibrin-rich technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve problems such as insufficient volume and insufficient strength, and achieve the effects of promoting biological activity, prolonging release time, and large volume

Inactive Publication Date: 2021-02-02
武汉齿欣生物医药科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the shortcomings of the existing solid-state platelet-rich fibrin preparation methods, the present invention provides an optimized preparation method for a solid-state platelet-rich fibrin membrane. The prepared platelet-rich fibrin membrane has larger volume, higher strength, and more active ingredients. It solves the problems of insufficient volume and insufficient strength of the current platelet-rich fibrin preparation products.

Method used

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  • Optimized preparation method of solid platelet-rich fibrin film
  • Optimized preparation method of solid platelet-rich fibrin film
  • Optimized preparation method of solid platelet-rich fibrin film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 Electric double layer treatment increases blood collection tube hydrophilicity

[0036] 1. Use a plasma surface treatment device to perform plasma treatment (300W, 13.56MHz, 3min) on the blood collection tube to remove the contamination impurities on the surface and make the surface negatively charged. Then soak the blood collection tube in the PAH polycation aqueous solution with a concentration of 0.01mol / L for 15 minutes, take it out and wash it with distilled water gradient for 3 times, each time for 2 minutes, and then soak the blood collection tube in the Ludox SM-30 aqueous solution with a concentration of 0.03% After 15 minutes, take it out and wash it with gradient distilled water for 3 times, 2 minutes each time. The above steps are one operation cycle, and one operation cycle will obtain a composite coating consisting of one layer of PAH cation layer and one layer of Ludox SM-30 anion layer on the glass surface, which is called an electric double...

Embodiment 2

[0040] Example 2 Concentrated sulfuric acid treatment increases the hydrophilicity of blood collection tubes

[0041] 1. The steps for treating glass tubes with concentrated sulfuric acid solution are as follows:

[0042] 1) Clean the glass tube ultrasonically with 75% ethanol for 10 minutes, then ultrasonically clean it 6 times with ultrapure water, and dry it with nitrogen;

[0043] 2) wash with ethanol and ultrapure water respectively, soak in concentrated sulfuric acid (95%-98%) for 12 hours, then ultrasonically clean with ultrapure water for 6 times, rinse with high pressure, and blow dry with nitrogen;

[0044] 3) Sterilized by gamma ray irradiation and packaged.

[0045] like image 3 As shown, after concentrated sulfuric acid treatment, the water contact angle on the glass surface changed. The contact angle before modification was 32°, but after concentrated sulfuric acid treatment, the contact angle was 11.2°, and the glass surface was hydrophilic.

[0046] 2. Vacu...

Embodiment 3

[0048] Embodiment 3 Piranha solution processing increases the hydrophilicity of blood collection tubes

[0049] 1. The steps of applying piranha solution to treat blood collection tubes are as follows:

[0050] 1) Clean the blood collection tube with 75% ethanol ultrasonically for 10 minutes, then ultrasonically clean it with ultrapure water for 6 times, and dry it with nitrogen gas;

[0051] 2) Prepare piranha solution with 98% concentrated sulfuric acid and 30% hydrogen peroxide solution according to the volume ratio of 7:1, soak the blood collection tube in the piranha solution for 12 hours, then ultrasonically clean it with ultrapure water for 6 times, and Rinse and blow dry with nitrogen;

[0052] 3) Vacuum the centrifugal blood collection tube, and put on the suitable butyl rubber stopper cap under the vacuum state;

[0053] 4) Sterilized by gamma ray irradiation and packaged.

[0054] To detect its water contact angle, such as Figure 5 As shown, after the piranha s...

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Abstract

The invention discloses an optimized preparation method of a solid platelet-rich fibrin film. The area and weight of the solid platelet-rich fibrin film can be effectively increased by improving the hydrophilicity of the tube wall of a blood collection tube, so that a fiber network in the film is more compact and has better toughness and tensile strength; meanwhile growth factors can be better released after the solid platelet-rich fibrin film is heated, the proportion of platelets and leukocytes in the solid platelet-rich fibrin film can be increased by properly increasing the tube diameter of the blood collection tube, the degradation time is effectively prolonged, and the problems of insufficient volume, insufficient strength and the like of an existing platelet-rich fibrin preparationproduct are solved; and the solid platelet-rich fibrin film prepared by the method has a wider application prospect clinically.

Description

technical field [0001] The invention belongs to the technical field of blood products, and in particular relates to an optimized preparation method of a solid platelet-rich fibrin membrane. Background technique [0002] The application of autologous blood extracts in wound healing and tissue regeneration is currently a research hotspot in bio-regeneration materials. It comes from itself, does not cause rejection of the body, and has a good regeneration effect. Blood is rich in components such as growth factors, fibrinogen, platelets and white blood cells. Growth factors such as platelet-derived growth factor (PGDF), transforming growth factor (TGF-β), vascular endothelial growth factor (VEGF), etc. are clinically used to accelerate the healing of soft and hard tissues; platelets play a variety of biological roles in the body On the one hand, it promotes blood coagulation; on the other hand, platelets are rich in various proteins that can promote wound healing; white blood c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/54A61L27/58
CPCA61L27/3616A61L27/3691A61L27/50A61L27/54A61L27/58A61L2300/414A61L2300/604A61L2430/02A61L2300/412
Inventor 张玉峰王宇蓝张晓欣理查德·麦伦
Owner 武汉齿欣生物医药科技有限责任公司
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