The invention provides a full-automatic PCB (printed circuit board) positioning clamp. The full-automatic PCB positioning clamp comprises a support seat, a support rod, a sliding support plate, a fixed-side railing, a movable-side railing, width adjusting mechanisms, conveying mechanisms, clamping mechanisms, a top plate mechanism, a detecting mechanism and a positioning mechanism, wherein the support rod and the width adjusting mechanisms are assembled on holes reserved on the lateral side of the support seat, the fixed-side railing is fixed on the support seat, the sliding support plate is slidably connected with the support rod, the movable-side railing is fixed on the sliding support plate which is connected with the width adjusting mechanisms, the conveying mechanisms are mounted on the inner sides of the fixed-side railing and the movable-side railing to form a PCB delivery rail, the clamping mechanisms are fixed on the fixed-side railing and the movable-side railing, the detecting mechanism is mounted on the fixed-side railing, and the positioning mechanism used for positioning the PCB when entering is fixed at a positioning position on the fixed-side railing. The full-automatic PCB positioning clamp can quickly automatically deliver the PCB, and can position and clamp the PCB accurately, reliably and conveniently, and production efficiency of an assembly line is improved.