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Traditional Chinese medicine maggot active peptide and preparation method and application thereof

A technology of grain worms and active peptides, which is applied in the field of medicine, can solve problems such as difficult to apply to the processing of large batches of samples, residues of extractants, waste of resources, etc., and achieve obvious effects of promoting wound healing, simple preparation methods, and improved utilization.

Active Publication Date: 2021-02-05
大连麦琪克生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent stimulates living grain worms to secrete active peptides and other substances, and collects them. Since the amount of peptides secreted by the grain worms is very small, it is difficult to obtain a large amount of active peptides by applying the method of this patent, and it is impossible to achieve industrialization. Require
The combined publication number is CN108392496A, and the patent name is "A Process for Extracting Fatty Acids from Chinese Medicine Grain Insects". This patent extracts the fatty acid components in the grain worms, and finally obtains the fatty acids and degreasing dregs of the grain worms. be well utilized, resulting in a waste of resources
The publication number is CN10394863, and the patent name is "Medical Use of Toad Feeder Antimicrobial Peptides". Although the patent involves the extraction method of antimicrobial peptides from cereal insects, this method needs to rely on centrifuges, and even requires the use of high-speed centrifuges for 15,000rpm extraction. Centrifugal operation, the investment of the centrifuge equipment is large, and the single processing volume of the high-speed centrifugation method is small, it is not easy to apply to the processing of large batches of samples. In addition, this method uses n-hexane extraction, n-hexane has anesthesia and stimulation effects, which is harmful to As far as the operator is concerned, long-term exposure can cause peripheral neuritis, and it also involves problems such as residual extractants and subsequent removal of impurities

Method used

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  • Traditional Chinese medicine maggot active peptide and preparation method and application thereof
  • Traditional Chinese medicine maggot active peptide and preparation method and application thereof
  • Traditional Chinese medicine maggot active peptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of traditional Chinese medicine five grain insect active peptide, comprising the following preparation steps:

[0025] (1) Salt extraction method: take an appropriate amount of dried traditional Chinese medicine Wuceinect degreased dregs, crush it through an 80-mesh sieve. Take 100g of the degreased dregs of the traditional Chinese medicine Wucea worm, add 500ml of 1% NaCl, stir at room temperature for 4h, centrifuge at 4000r / min for 10min, and take the supernatant. Repeat the above process and combine the 2 supernatants. Take the supernatant, add 10% HCl to adjust the pH to 6-7, a precipitate can be seen, and centrifuge at 4000r / min for 10min. The precipitate was dialyzed by the dialysis method, and after dialysis for 24 hours, centrifuged at 4000 r / min for 10 minutes, the precipitate was taken and freeze-dried to obtain protein components. According to the determination requirements of protein content in Pharmacopoeia, Kjeldahl method was applie...

Embodiment 2

[0028] A preparation method of traditional Chinese medicine five grain insect active peptide, comprising the following preparation steps:

[0029] (1) Salt extraction method: take an appropriate amount of degreased dregs of the dried traditional Chinese medicine Wucea worm, crush them through a 60-mesh sieve. Take 200g of the degreased dregs of the traditional Chinese medicine Wucea worm, add 1L of 2% NaCl, stir at room temperature for 4h, centrifuge at 4500r / min for 20min, and take the supernatant. Repeat the above process and combine the 2 supernatants. Take the supernatant, add 10% phosphoric acid to adjust the pH to 6-7, a precipitate can be seen, and centrifuge at 4500r / min for 20min. The precipitate was dialyzed by dialysis. After dialysis for 24 hours, centrifuged at 4500 r / min for 20 minutes, the precipitate was taken and freeze-dried to obtain 140.9 g of Chinese medicine grain insect protein (accounting for 70.5% of the degreased medicinal residue).

[0030] (2) Enz...

Embodiment 3

[0032] A preparation method of traditional Chinese medicine five grain insect active peptide, comprising the following preparation steps:

[0033] (1) Salt extraction method: take an appropriate amount of dried traditional Chinese medicine Wuceinect degreased dregs, crush it through an 80-mesh sieve. Take 500g of the degreased dregs of the traditional Chinese medicine Wucea worm, add 2.5L of 1% NaCl, stir at room temperature for 4h, centrifuge at 5000r / min for 10min, and take the supernatant. Repeat the above process and combine the 2 supernatants. Take the supernatant, add 10% HCl to adjust the pH to 6-7, a precipitate can be seen, and centrifuge at 5000r / min for 10min. The precipitate was dialyzed by dialysis. After dialysis for 24 hours, centrifuged at 5000 r / min for 10 minutes, the precipitate was taken and freeze-dried to obtain 330.6 g of Chinese medicine grain insect protein (accounting for 66.1% of the degreased medicinal residue).

[0034] (2) Enzymolysis method: ge...

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PUM

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to a traditional Chinese medicine maggot (dried larva of chrysomyia megacephala) active peptide. The invention further relates to a preparation method and application of the traditional Chinese medicine maggot active peptide. The traditional Chinese medicine maggot active peptide can be prepared by the following method of taking dry traditional Chinese medicine maggot degreased dregs; adding NaCl of which the mass is 5-8 times that of the maggot degreased dregs, stirring for 4-5 hours at room temperature, centrifuging and taking supernatant, wherein the concentration of NaCl is 1-2wt%; and adjusting pH, separating out precipitate, dialyzing, centrifuging, taking precipitate, freeze-drying to obtain traditional Chinese medicine maggot protein, preparing a solution with the substrate concentration of 7-13%, adding pepsase accounting for 0.5-1.5 wt% of the mass of the traditional Chinese medicine maggot protein, extracting at 35-40 DEG C, heating to boil, cooling, filtering, freeze-drying to obtain a product. According to the method, the resource utilization of the traditional Chinese medicine maggot is improved, the raw material cost is reduced, the degreasing step in the preparation process of the traditional Chinese medicine maggot active peptide is reduced, and the yield of the traditional Chinese medicine maggot active peptide is remarkably improved.

Description

technical field [0001] The invention belongs to the technical field of medicine; in particular, it relates to a traditional Chinese medicine five grain insect active peptide; and also relates to a preparation method and application of the traditional Chinese medicine five grain insect active peptide. Background technique [0002] Grain worms are insects of the family Muscidae or Calliphoridae. According to the records in "Materia Medica Qiuyuan" and "Ben Cao Bian Du", the grain worms are cold in nature. After drying and grinding, external application can treat ecchymosis and lip boils. Traditional Chinese medicine believes that grain worms are salty and cold in nature, non-toxic, and have the functions of clearing away heat and eliminating stagnation, detoxification and anti-inflammatory, removing putrefaction and promoting muscle growth, and can treat infectious surgical diseases such as ecchymosis. At present, grain worms are allowed to be used clinically in the United Sta...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14C07K1/30A61K38/01A61K35/64A61P17/02A61P3/10A23L33/18A23L33/17
CPCC12P21/06C07K1/145C07K1/30A61K38/012A61K35/64A61P17/02A61P3/10A23L33/18A23L33/17A23V2002/00A23V2200/328A23V2200/30A23V2250/55A23V2250/542Y02A50/30
Inventor 李继和
Owner 大连麦琪克生物技术有限公司
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