Method for extracting bile acid by using ox bile efficient enzyme

Abstract

The invention discloses a method for extracting bile acid by using ox bile efficient enzyme, and belongs to the technical field of animal bile effective extraction. The method comprises the followingsteps of mixing raw materials and a compound enzyme preparation, adjusting the pH value, carrying out enzymolysis and stirring adjustment, and carrying out solid-liquid separation to obtain an extracting solution; carrying out enzyme deactivation, adding bile salt hydrolase for enzymolysis, adjusting the pH value, performing heating, performing stirring saponification, performing centrifugation, and adjusting the pH value to obtain a supernatant; adding hydrogen peroxide, performing uniform stirring and mixing, reacting at room temperature, and performing filtration to obtain a treatment solution; slowly adding butyl acetate, performing stirring, then adjusting the pH value of the solution, and carrying out solid-liquid separation to obtain solid matter; and performing centrifugation and water washing, and performing drying until the water content is not higher than 1%, thereby obtaining finished bile acid extract. According to the invention, the enzyme preparation is adopted for extraction, so that the product purity is improved, impurities are reduced, the product yield is increased, pollution caused by participation of toxic substances is fundamentally avoided, and the problem of environmental pollution caused by strong acid and strong alkali is reduced; and the method is simple in process, low in investment and low in cost.

Description

technical field [0001] The invention relates to the technical field of effective extraction of animal bile, and more specifically relates to a method for extracting bile acid with high-efficiency enzymes from ox bile. Background technique [0002] Bovine bile is the bile of bovine cattle or buffalo. The color of fresh bile is greenish brown or dark brown and slightly transparent. It is slightly sticky, fishy and bitter. It is one of the main raw materials for extracting CA and DCA. Bile is a mixture of various components. In addition to a large amount of water, it mainly contains bile acids, bile pigments, cholesterol, fatty acids, lecithin, major and trace elements, vitamins, amino acids, inorganic salt ions, etc., similar to body fluids; In addition, it also contains metabolites such as urea and purine, thyroxine, sex hormones, and immune proteins. The content of bile acids in bile accounts for 50% to 60%, and is one of the main components of bile. Bile acids are the gen...

AI Technical Summary

Problems solved by technology

[0005] At present, the bile of cattle, sheep, and pigs is generally separated and extracted at home and abroad. There are mainly three extraction methods: ethanol crystallization method, chloroform method, ethyl acetate separation method; chloroform method, ethanol crystallization method, ethyl acetate separation method , but, above-mentioned method all has poisonous substance (chloroform, ethyl acetate) to participate in, and in order to reduce impurity need cause protein denaturation, fatty acid saponification etc. with ethanol, strong base or strong acid, reagent consumption is big, cost is higher, and yield Low, low purity, impurity content is still high

[0006] If microbial treatment is used, since bile acids are raw materials for enterohepatic circulation in animals, the contents of the ileum and large intestine of animals are extracted to culture and extract the microorganisms to hydrolyze the bound bile acids into free bile acids, which is not suitable for industrial production, resulting in The process is cumbersome and the cost increases