Method for trapping soil microorganisms

Abstract

The invention discloses a method for trapping soil microorganisms. The method comprises the following steps: firstly, carrying out microorganism culture, putting soil into a glass culture dish, wetting the soil with distilled water, manufacturing a microorganism trapping device containing a solid culture medium, putting the device into the wet soil in the culture dish, manufacturing a microorganism culture device, and putting the microorganism culture device into an incubator for culturing; and then carrying out microorganism separation, mashing the solid culture medium, adding sterile water for standing, carrying out gradient dilution, coating an oligotrophic culture medium with a bacterium solution, carrying out blow-drying and culturing in the incubator, observing bacterial colony growth, and carrying out storage after purification and identification. According to the method, the soil microorganisms are trapped and domesticated in the soil through the solid culture medium, so that the soil microorganisms including bacteria which are difficult to culture and low in propagation speed can be obtained, and the separation depth of the soil microorganisms is expanded. The microorganisms obtained with the method can be used for morphological observation, classification and identification of the microorganisms, plant disease control, pesticide degradation, environmental protection and the like.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a method for trapping soil microorganisms. Background technique [0002] The soil has the nutrients, water, air, pH, osmotic pressure, temperature and other conditions required for the growth and development of various microorganisms, so the soil is a good environment for microorganisms to live, and contains almost all microbial populations, so the soil is the most abundant. "Strain Resource Library". The amount of microorganisms contained in the soil is huge, especially bacteria. It is estimated that each gram of soil contains 4000-7000 different bacterial species, with a total of about 1 billion (Yanyan, Chen Jian, Yanchangjiang, etc. using molecular biology Methods for technical identification of soil microorganisms [J]. Journal of Northeast Agricultural University, 2008,39(003):129-133.). [0003] Soil microorganisms are rich in species, but due to the lim...

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Problems solved by technology

For example, when nutrient-rich beef extract peptone was used as a medium to isolate soil microorganisms in the laboratory, a large number of nutrient-poor microorganisms were not suitable for growth.

[0006] Secondly, due to the dilution of sterile water in the conventional plate culture method, the necessary growth factors in the soil sample are lost during the dilution process, making it difficult to cultivate some special microorganisms.

[0007] Therefore, it is impossible to comprehensively obtain all or most of the microbial information in the soil by using the plate culture method.

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