Complex microbial agent used for treating high ammonia nitrogen wastewater and preparation method thereof
A compound microbial agent, high ammonia nitrogen technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of ineffective improvement of water body material circulation, high operating cost of activated sludge method, and microbial strains Single problem, to achieve the effect of digestion of pollutants and organic matter in river bottom sludge, enhancement of ammonia nitrogen degradation ability and stability, and short treatment time
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[0020]Example 1
[0021]A composite microbial inoculum for treating sewage with high ammonia nitrogen and a preparation method thereof. The composite microbial inoculum includes Shewanella putrefaction, Pseudomonas testosterone, nitrifying bacteria, nitrosating bacteria, Bacillus subtilis, Lactomicrobe, Rhodococcus faecalis, yeast, actinomycetes and photosynthetic bacteria, the specific steps of the preparation method are:
[0022](A) Solid slant culture: inoculate nitrifying bacteria, nitrosating bacteria and Bacillus subtilis in nitrifying solid medium, nitrosating solid medium and beef extract peptone medium, respectively, and cultivate them to fully activate the strains;
[0023](B) Inoculate the activated nitrifying bacteria, nitrosating bacteria and Bacillus subtilis in step (a) into nitrifying liquid medium, nitrosating liquid medium and beef extract peptone liquid medium, respectively, when the liquid culture density is OD600 When the value reaches 0.6-0.8, stop the cultivation to fo...
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[0032]Example 2
[0033]A composite microbial inoculum for treating sewage with high ammonia nitrogen and a preparation method thereof. The composite microbial inoculum includes Shewanella putrefaction, Pseudomonas testosterone, nitrifying bacteria, nitrosating bacteria, Bacillus subtilis, Lactomicrobe, Rhodococcus faecalis, yeast, actinomycetes and photosynthetic bacteria, the specific steps of the preparation method are:
[0034](A) Solid slant culture: inoculate nitrifying bacteria, nitrosating bacteria and Bacillus subtilis in nitrifying solid medium, nitrosating solid medium and beef extract peptone medium, respectively, and cultivate them to fully activate the strains;
[0035](B) Inoculate the activated nitrifying bacteria, nitrosating bacteria and Bacillus subtilis in step (a) into nitrifying liquid medium, nitrosating liquid medium and beef extract peptone liquid medium, respectively, when the liquid culture density is OD600 When the value reaches 0.6-0.8, stop the cultivation to fo...
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