Complex microbial agent used for treating high ammonia nitrogen wastewater and preparation method thereof
A compound microbial agent, high ammonia nitrogen technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of ineffective improvement of water body material circulation, high operating cost of activated sludge method, and microbial strains Single problem, to achieve the effect of digestion of pollutants and organic matter in river bottom sludge, enhancement of ammonia nitrogen degradation ability and stability, and short treatment time
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Embodiment 1
[0021] A compound microbial agent for treating high ammonia nitrogen sewage and a preparation method thereof, the compound microbial agent includes Shewanella putrefaciens, Pseudomonas testosterone, nitrifying bacteria, nitrosifying bacteria, Bacillus subtilis, Lactobacillus, Rhodococcus faecalis, saccharomyces, actinomycetes and photosynthetic bacteria, the specific steps of the preparation method are:
[0022] (a) Solid slant culture: Inoculate nitrifying bacteria, nitrosifying bacteria and Bacillus subtilis in nitrifying solid medium, nitrosifying solid medium and beef extract peptone medium for culture respectively to fully activate the strains;
[0023] (b) Inoculate the nitrifying bacteria, nitrosifying bacteria and Bacillus subtilis activated in step (a) in nitrifying liquid medium, nitrosifying liquid medium and beef extract peptone liquid medium for culture respectively, when the density of liquid culture bacteria is OD600 When the value reaches 0.6-0.8, stop cultivat...
Embodiment 2
[0033] A compound microbial agent for treating high ammonia nitrogen sewage and a preparation method thereof, the compound microbial agent includes Shewanella putrefaciens, Pseudomonas testosterone, nitrifying bacteria, nitrosifying bacteria, Bacillus subtilis, Lactobacillus, Rhodococcus faecalis, saccharomyces, actinomycetes and photosynthetic bacteria, the specific steps of the preparation method are:
[0034] (a) Solid slant culture: Inoculate nitrifying bacteria, nitrosifying bacteria and Bacillus subtilis in nitrifying solid medium, nitrosifying solid medium and beef extract peptone medium for culture respectively to fully activate the strains;
[0035] (b) Inoculate the nitrifying bacteria, nitrosifying bacteria and Bacillus subtilis activated in step (a) in nitrifying liquid medium, nitrosifying liquid medium and beef extract peptone liquid medium for culture respectively, when the density of liquid culture bacteria is OD600 When the value reaches 0.6-0.8, stop cultivat...
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