Proliferation culture medium for human skin fibroblasts and preparation method thereof
A technology for fibroblasts and culture medium, applied in the field of culture medium, can solve the problems of serum safety hazards, complex serum components, low cell proliferation rate, etc., achieves simple and easy operation in the preparation process, improves cell growth rate, and improves proliferation efficiency. Effect
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Embodiment 1
[0021] An expansion medium for human skin fibroblasts, consisting of DMEM / F12 medium and additives, the concentration of the additives in the DMEM / F12 medium is: insulin 45 μg / mL, phytic acid 18 μg / mL, Germinated brown rice powder 20 μg / mL, feruloylglycan 15 ng / mL, nano zinc oxide with an average particle size of 20 nm ng / mL, chitin 15 μg / mL, sodium selenite 18 μg / mL.
[0022] The preparation method of the above-mentioned expansion medium for human skin fibroblasts comprises the following steps: taking the basal medium and adding insulin, phytic acid, germinated brown rice flour, feruloylglycan, nano-zinc oxide, chitin, and selenous Sodium acid, mix evenly, and use 0.22μm microporous membrane to filter and sterilize.
Embodiment 2
[0024] An expansion medium for human skin fibroblasts, consisting of DMEM / F12 medium and additives, the concentration of the additives in the DMEM / F12 medium is: insulin 50 μg / mL, phytic acid 20 μg / mL, Germinated brown rice flour 25 μg / mL, feruloylglycan 18 ng / mL, nano zinc oxide with an average particle size of 20 nm 8 ng / mL, chitin 17 μg / mL, sodium selenite 20 μg / mL.
[0025] The preparation method of the expansion medium for human skin fibroblasts is the same as in Example 1.
Embodiment 3
[0027] An expansion medium for human skin fibroblasts, consisting of DMEM / F12 medium and additives, the concentration of the additives in the DMEM / F12 medium is: insulin 55 μg / mL, phytic acid 25 μg / mL, Germinated brown rice flour 27μg / mL, feruloylglycan 20ng / mL, nano zinc oxide with an average particle size of 50nm 10ng / mL, chitin 20μg / mL, sodium selenite 21μg / mL.
[0028] The preparation method of the expansion medium for human skin fibroblasts is the same as in Example 1.
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