Glycosylase biosensor based on carboxylated nano-zinc oxide and preparation method and application thereof

A nano-zinc oxide, biosensor technology, applied in instruments, scientific instruments, electrochemical variables of materials, etc., can solve the problems of long detection time, low selectivity, complex instruments, etc., achieve low detection limit, simple preparation, anti-interference powerful effect

Active Publication Date: 2021-03-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional determination methods use complex instruments, long

Method used

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  • Glycosylase biosensor based on carboxylated nano-zinc oxide and preparation method and application thereof
  • Glycosylase biosensor based on carboxylated nano-zinc oxide and preparation method and application thereof
  • Glycosylase biosensor based on carboxylated nano-zinc oxide and preparation method and application thereof

Examples

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preparation example Construction

[0045] The preparation of nano zinc oxide used in the present invention is as follows:

[0046] (1) Add 1mol / L (NH 4 ) 2 CO 3 The solution was added dropwise to 1mol / L ZnSO 4 In the solution, stir while dropping until pH = 8, then heat and react in a water bath at 60°C for 1 h;

[0047] (2) After the reaction is completed, repeatedly wash and filter the reaction solution with distilled water until no SO is detected in the filtrate. 4 2- So far (with BaCl 2 test);

[0048] (3) Dry the obtained white product in a vacuum oven at 110°C for 10 hours to obtain a zinc oxide precursor;

[0049] (4) Calcining the obtained zinc oxide precursor in a high-temperature tube furnace at 500° C. for 2 hours, and then the nano-zinc oxide material can be prepared.

Embodiment 1

[0051] A method for preparing a glycerase biosensor based on carboxylated nano zinc oxide, comprising the following steps:

[0052] (1) Weigh 2.5g of nano-zinc oxide in a conical flask, add 60g of distilled water for ultrasonic dispersion for 4 hours; In the three-necked flask, add 1.25g vinyltriethoxysilane subsequently, and heat the reaction in a water bath at a temperature of 85°C for 2h; Dissolve 0.73g of ammonium persulfate and 0.18g of sodium bisulfite in distilled water), and continue to react in a water bath at 85°C for 3.5h; Carboxylated nano zinc oxide material.

[0053] (2) A gold electrode with a diameter of 3 mm is sequentially covered with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder was polished to a mirror surface, rinsed with distilled water; then ultrasonically cleaned in absolute ethanol and distilled water for 2 minutes, taken out and washed with distilled water; 2 SO 4 In the solution, scan to stability by cyclic voltammetry at -0.1 ~ 1.2...

Embodiment 2

[0062] A method for preparing a glycerase biosensor based on carboxylated nano zinc oxide, comprising the following steps:

[0063] (1) Weigh 2.5g of nano-zinc oxide in a conical flask, add 60g of distilled water for ultrasonic dispersion for 4 hours; In the three-necked flask, add 1.25g vinyltriethoxysilane subsequently, and heat the reaction in a water bath at a temperature of 85°C for 2h; Dissolve 0.73g of ammonium persulfate and 0.18g of sodium bisulfite in distilled water), and continue to react in a water bath at 85°C for 3.5h; Carboxylated nano zinc oxide material.

[0064] (2) A gold electrode with a diameter of 3 mm is sequentially covered with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water; then ultrasonically cleaned in absolute ethanol and distilled water for 2 minutes, taken out and washed with distilled water; then placed in 10 mL of 1mol / L H 2 SO 4 In the solution, scan to stability by ...

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Abstract

The invention belongs to the technical field of electrochemical biosensors, and particularly discloses an enzyme biosensor for detecting glycerol based on carboxylated nano-zinc oxide and a preparation method and application thereof. The enzyme biosensor adopts a classical three-electrode system, a specific substance recognition film is solidified on a working electrode, and the substance recognition film is mainly prepared by mixing a carboxylated zinc oxide material layer, a glycerol kinase solution, a glycerol triphosphate oxidase solution and a chitosan solution. A three-electrode system is formed by using the modified working electrode, a reference electrode and a counter electrode so as to obtain the enzyme biosensor for detecting glycerol. The enzyme biosensor for detecting glycerolhas good electron transmissibility, can well transfer electrons generated by reaction, can realize selective detection of biomolecules, improves the reaction speed of the biosensor, and has good selectivity, reproducibility and stability.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensors, and in particular relates to an enzyme biosensor for detecting glycerin based on carboxylated nano-zinc oxide and its preparation method and application. Background technique [0002] Glycerol, also known as 1,2,3-propanetriol, is one of the main by-products in the early stages of wine fermentation. Because of its sweetness and viscosity, a certain amount of glycerin can weaken the bitterness of wine and make wine taste mellow, which is one of the important indicators for evaluating wine quality. During the normal fermentation process of wine, the glycerol content produced by it is usually 4-15g / L, and the liquor ratio is 6%-10%. In order to make more profits, some unscrupulous merchants may add glycerin to the blended wine to improve the taste of the wine, which is strictly prohibited in our country and other countries. At present, the methods for detecting glycerol include:...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/327G01N27/48
Inventor 朴金花肖焱堃
Owner SOUTH CHINA UNIV OF TECH
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