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Preparation method of novel red yeast rice powder with blood fat reducing function

A technology of red yeast rice powder and blood lipid lowering, which is applied in the field of bioengineering and health care product preparation, can solve problems such as structural instability, and achieve low-cost effects

Pending Publication Date: 2021-03-12
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing functional red yeast rice is mainly rich in Monacolin K. Monacolin K has a better effect in lowering blood lipids, but its structure is unstable and easily converted into a lactone structure

Method used

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  • Preparation method of novel red yeast rice powder with blood fat reducing function
  • Preparation method of novel red yeast rice powder with blood fat reducing function
  • Preparation method of novel red yeast rice powder with blood fat reducing function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the screening of strain

[0025] Taking several strains of Monascus strains (Z505, GN, H4000, etc.) with strong esterification ability in the laboratory as the research object, the slant seed activation, liquid seed cultivation and liquid fermentation culture of Monascus were carried out to obtain the red yeast. Aspergillus fermentation broth was filtered, freeze-dried and pulverized to obtain functional red yeast rice powder, and the content of ethyl linoleate was determined by gas chromatography-mass spectrometry.

[0026] Slope medium: glucose 6g, peptone 2g, soluble starch 3g, agar 3g, heated and dissolved in 100mL water, adjusted to pH 5.38 with lactic acid, distributed in test tubes, about 8mL / tube, sterilized at 115°C for 30min.

[0027] Secondary seed medium: Weigh 10g of fresh sweet potato pieces cut into small pieces, 2g of glucose, 0.6g of peptone, 0.3g of potassium dihydrogen phosphate, 0.2g of magnesium sulfate, add 100mL of water, adjust the ...

Embodiment 2

[0036] Slant culture medium: the same as in Example 1.

[0037] Secondary seed medium: 2g of raw material, 1g of soybean powder, 2g of glucose, 1g of peptone, add 100mL of water, adjust the pH value between 5.3-5.6 with lactic acid, and sterilize at 115°C for 30min.

[0038] Fermentation medium: 5g of raw materials, 2g of soybean flour, 0.05g of magnesium sulfate, 0.5g of potassium dihydrogen phosphate, add 100mL of water, adjust the pH value between 5.5-5.8 with lactic acid, and sterilize at 121°C for 20min.

[0039]The grain raw materials are respectively rice flour, barley flour, black rice flour, purple potato flour, sweet potato flour and fresh sweet potato, and the grain raw materials in the secondary seed culture medium and the fermentation medium are the same. According to the method in Example 1, the obtained secondary seeds were inserted into the fermentation medium with an inoculum size of 10% (V / V), and fermented and cultivated for 7 days at 20°C, 200rpm and 30°C, ...

Embodiment 3

[0044] (1) Slant culture medium: the same as in Example 1.

[0045] (2) Inoculate Monascus spp. and carry out slant culture: Inoculate Monascus purple Z505 into the slant medium, and culture at 30° C. for 7 days to obtain first-grade seeds.

[0046] (3) Preparation of secondary seed medium: Weigh 2 g of rice flour, 1 g of soybean flour, 1 g of glucose, 1 g of peptone, 0.5 g of potassium dihydrogen phosphate, and 0.05 g of magnesium sulfate, add 100 mL of water, and adjust the pH value to 5.43, 121 with lactic acid. Sterilize at ℃ for 20min.

[0047] (4) Inoculate Monascus, and carry out secondary seed cultivation: scrape the primary seeds in the slant medium and inoculate them in the secondary seed medium, and vibrate at 30° C. and 200 rpm for 2 days to obtain secondary seeds.

[0048] (5) Preparation of fermentation medium: Weigh 5 g of rice flour, 1 g of soybean flour, 0.5 g of ammonium sulfate, 0.5 g of potassium dihydrogen phosphate, 0.05 g of magnesium sulfate, 0.05 g of...

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Abstract

The invention discloses a preparation method of novel red yeast rice powder with a blood fat reducing function, which belongs to the technical field of bioengineering and health care product preparation. The method disclosed by the invention comprises the following steps: inoculating a monascus purpureus Z505 seed solution into a fermentation medium for culturing, and filtering, freeze-drying andcrushing the obtained fermentation liquor to obtain the functional red yeast rice powder with the blood fat reducing function. The formula of the fermentation medium comprises 4 to 8% of cereals, 1 to3% of soybean meal, 0.2 to 1% of ammonium sulfate, 0.1 to 1% of monopotassium phosphate, 0.01 to 0.1% of magnesium sulfate, 0.01 to 0.1% of potassium chloride, 0.0001 to 0.001% of ferrous sulfate, 0.001 to 0.005% of manganese sulfate, 0.0001 to 0.001% of zinc sulfate and the balance of water, and the pH value of the fermentation medium is 5 to 6. The content of ethyl linoleate in the obtained functional red yeast rice powder is about 15 mg / g, and the functional red yeast rice powder can be used in food and health care products.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and preparation of health care products, and in particular relates to a preparation method of novel red yeast rice powder with hypolipidemic function. Background technique [0002] Ethyl linoleate is a colorless to pale yellow oily liquid that can lower blood cholesterol and blood lipids. It is mainly used as a pharmaceutical raw material to prevent and treat atherosclerosis. Ethyl linoleate is structurally similar to linoleic acid, and has the functions of lowering blood lipids, softening blood vessels, lowering blood pressure, and promoting microcirculation, but direct consumption of linoleic acid can irritate the throat, while ethyl linoleate is derived from linoleic acid The esters formed with lower alcohols are easily hydrolyzed in the body, and the organic esters are stable and easier to store. [0003] At present, the production methods of ethyl linoleate are mainly prepared by chem...

Claims

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Application Information

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IPC IPC(8): A23L33/135C12N1/14C12P7/64C12R1/645
CPCA23L33/135C12N1/14C12P7/6436A23V2002/00A23V2200/3262
Inventor 王伟平况嘉铀韦胜
Owner HUBEI UNIV OF TECH
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