A kind of biologically active peptide lecvepncrskr and its preparation method and application

A technology of biologically active peptides and sequences, applied in the field of proteins, to achieve the effects of inhibiting overexpression, improving quality, and inhibiting inflammation

Active Publication Date: 2022-03-29
ZHEJIANG PANDA DAIRY GRP COMPANY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the function of the polypeptide fragment of 60S ribosomal protein L36a protein in the prior art

Method used

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  • A kind of biologically active peptide lecvepncrskr and its preparation method and application
  • A kind of biologically active peptide lecvepncrskr and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The artificial synthesis of embodiment 1 active peptide LECVEPNCRSKR

[0032] 1. Synthesis of Bioactive Peptides

[0033] Synthetic bioactive peptide LECVEPNCRSKR.

[0034] 2. Confirmation of Bioactive Peptides

[0035] 1) UPLC analysis

[0036] The UPLC conditions are as follows:

[0037] Instruments: Waters ACQUITY UPLC ultra-high performance liquid phase, electrospray, quadrupole, time-of-flight mass spectrometer

[0038] Column specification: BEH C18 column

[0039] Flow rate: 0.4mL / min

[0040] Temperature: 50°C

[0041] UV detection wavelength: 210nm

[0042] Injection volume: 2μL

[0043] Gradient conditions: liquid A: water containing 0.1% formic acid (v / v), liquid B: acetonitrile containing 0.1% formic acid (v / v)

[0044] Time(min) %A %B

0 95.0 5.0

1.50 80.0 20.0

3.50 60.0 40.0

5.00 40.0 60.0

7.00 15.0 85.0

8.00 0.0 100.0

11.00 0.0 100.0

11.50 95.0 5.0

13.00 95.0 5.0

[0045] 2) Mass spectrometry analysis

[0046] The mass spectrometr...

Embodiment 2

[0060] Example 2 Immune Activity Experiment of Bioactive Peptides

[0061] 1. Determination of the amount of nitric oxide induced by the bioactive peptide LECVEPNCRSKR in macrophages (Griess method)

[0062] 1. Experimental reagents and instruments:

[0063] Reagents: experimental animals balb / c mice (male 6-8 weeks old) spleen lymphocyte-derived bioactive peptide LECVEPNCRSKR; LPS, purchased from Sigma; neutral red staining solution, produced by Beyond Biotechnology Research Institute.

[0064] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150 CO 2 Incubator Heraeus; DragonWellscan MK3 microplate reader Labsystems.

[0065] 2. Test method:

[0066] The number of cells added is 2×10 6 / ml cell suspension 100μl / well, after adherent purification, add 200μl / well of RPMI1640 complete culture solution (10% FBS) containing peptide, add LPS to th...

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Abstract

The present invention relates to the field of proteins, in particular to a bioactive peptide LECVEPNCRSKR and its preparation method and application. The amino acid sequence of the bioactive peptide LECVEPNCRSKR is Leu-Glu-Cys-Val-Glu-Pro-Asn-Cys-Arg-Ser-Lys ‑Arg. Through the in vitro immune function regulation experiments, it was verified that the biologically active peptide LECVEPNCRSKR has a good immune regulation function. The biologically active peptide LECVEPNCRSKR of the present invention has the ability to increase the induction of macrophage nitric oxide under a certain concentration, can effectively inhibit the inflammation caused by oxidation in the body, inhibit the overexpression of inflammatory factors, and improve the body's resistance to external pathogens The ability to infect, reduce the morbidity of the body, and improve the quality of life are of great significance to the development of foods, health products and medicines with immune regulation functions.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a bioactive peptide LECVEPNCRSKR and its preparation method and application. Background technique [0002] At present, most studies on bioactive peptides focus on food-derived peptides, while there are few studies and reports on non-food-derived peptides. And it has been confirmed by research results that, compared with food-derived bioactive peptides, non-food-derived bioactive peptides have higher affinity and can effectively exert their bioactive functions. Lymphocytes are the central regulatory cells of the immune system, and most of their functions are mediated by a group of small molecule polypeptides called lymphokines. Both the expression and secretion of these small molecule polypeptides are induced by antigen-stimulated cell activation. Therefore, lymphocytes are the main source of immunomodulatory peptides produced in animals. [0003] Immunomodulatory peptides are a class o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12A61K8/64A61K38/17A61Q19/00A61P29/00A61P37/02A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61P29/00A61P37/02A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2250/55
Inventor 张少辉肖珊珊石毅洪志骏王娟李锡安占文静陈承余梁世排梁世赞
Owner ZHEJIANG PANDA DAIRY GRP COMPANY
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