Bacillus velezensis BMF 03 water suspending agent as well as preparation method and application thereof

A technology of Bacillus velesi and water suspension agent, which is applied in the field of inoculants, can solve the problems of reducing the nutritional value of pears, causing pesticide residues in fruits and pathogenic bacteria, and environmental pollution.

Active Publication Date: 2021-03-16
JIANGSU OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The quality of fresh fruit is affected by many factors, among which spoilage caused by pathogenic microorganisms is one of the most important reasons for fruit loss, which not only reduces the nutritional value of pears, but also affects its economic benefits.
At present, the prevention and treatment of postharvest pear diseases mainly rely on chemical methods, and problems such as environmental pollution caused by extensive use and resistance of fruit pesticide residue pathogenic bacteria are becoming more and more serious.

Method used

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  • Bacillus velezensis BMF 03 water suspending agent as well as preparation method and application thereof
  • Bacillus velezensis BMF 03 water suspending agent as well as preparation method and application thereof
  • Bacillus velezensis BMF 03 water suspending agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, Bacillus velezensis (Bacillus velezensis) BMF 03 water suspension preparation experiment:

[0045] 1.1 Materials and methods

[0046] 1.1.1 Tested strains

[0047] Bacillus velezensis BMF 03 strain: It was isolated and preserved from Lianyungang sea area in our laboratory.

[0048] 1.1.2 Medium

[0049] Strain activation medium: PDA medium; seed liquid medium, fermentation medium: PD medium.

[0050] 1.1.3 Additives

[0051] Table 1-1 Types of additives

[0052]

[0053] 1.1.4 Preparation of BMF 03 strain seed solution

[0054] After activating the BMF 03 strain, inoculate one ring into a 250mL Erlenmeyer flask filled with 60mL PD medium, culture at 28°C and shake at 180r / min for 16h; adjust the concentration of the bacterial solution to 10 8 cells / mL as seed solution for fermentation.

[0055] 1.1.5 Preparation of fermentation broth

[0056] The BMF 03 strain seed solution was inoculated into a 250mL Erlenmeyer flask with 60mL PD medium accordi...

Embodiment 2

[0169] Embodiment 2, the prevention and control effect experiment of Bacillus bacillus BMF 03 water suspension to pear ring spot:

[0170] In this experiment, the indoor toxicity of BMF 03 strain water suspension to Pear Ringworm and its control effect on Pear Ringworm were determined, which provided a theoretical basis for the use of the strain preparation in the prevention and control of pear postharvest diseases.

[0171] 2.1 Materials and methods

[0172] 2.1.1 Tested strains

[0173] Bacillus velezensis BMF 03 strain was isolated from Lianyungang waters; Botryosphaeria dothidea was isolated and preserved in our laboratory.

[0174] 2.1.2 Medium

[0175] PDA medium: 200g potato, 20g glucose, 20g agar, 1000mL water;

[0176] PD medium: 200g potato, 20g glucose, 1000mL water.

[0177] 2.1.3 Activation of BMF 03 strain and pathogenic fungi

[0178] The BMF 03 strain and R. pearensis preserved at 4°C were respectively transferred to the PDA medium slant and plate under as...

Embodiment 3

[0206] Embodiment 3, the measurement experiment of the Veles bacillus BMF 03 water suspension concentrate performance that embodiment 1 makes:

[0207] 3.1 Determination of thermal storage stability of BMF 03 strain water suspension

[0208] (1) Take the prepared BMF 03 strain water suspension and store it in an oven at 54±2°C for 14 days, and measure the total number of bacteria, suspension rate and fluidity before and after storage; the pH of the BMF 03 strain water suspension is determined according to the national standard GB / T 1601 Methods.

[0209] (2) Store the prepared BMF 03 bacterial strain water suspensions at 4°C and normal temperature conditions respectively, take samples every 10 days for the first 50 days, take samples every 30 days after 50 days, and measure the colonies in the preparation by plate colony counting method number, miscellaneous bacteria rate and suspension rate.

[0210] (3) Results

[0211] BMF 03 strain water suspension was tested for heat s...

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Abstract

The invention relates to a bacillus velezensis BMF03 water suspending agent, which is composed of bacillus velezensis BMF03 fermentation broth and an auxiliary agent, and the auxiliary agent comprisesthe following components in percentage by weight: 1.5% of wetting dispersant calcium lignosulphonate, 0.25% of a viscosity modifier xanthan gum; 0.6% of an antifoaming agent soybean oil; 0.8% of preservative sodium chloride; and 0.2% of an ultraviolet protective agent dextrin. The thallus density of the water suspending agent is not lower than 2*10<9>CFU/mL. The invention further discloses a preparation method of the bacillus velezensis BMF03 water suspending agent. The bacillus velezensis BMF03 water suspending agent can be used for preparing a medicine for treating pear ring rot, the BMF 03strain fermentation liquor and the water suspending agent have good prevention and treatment effects on the pear ring rot, the prevention and treatment effects are 63.94% and 69.14% respectively, andthe prevention and treatment effect of the preparation is higher than that of the fermentation liquor.

Description

technical field [0001] The present invention relates to a kind of bacterial agent, particularly a kind of bacillus veles BMF 03 water suspension; The present invention also relates to the preparation method and the application of veles bacillus BMF 03 water suspension. Background technique [0002] Marine bacteria grow rapidly and have abundant metabolites, and have become an important microbial resource for the biological control of plant diseases. Several excellent strains of marine bacteria with strong antibacterial effects have been isolated by different scholars. Zhu Xiaofei and others screened the strain YB-3, which has a strong antibacterial effect on rice sheath blight, and was identified as Bacillus amyloliquefaciens by morphological observation, physiological and biochemical tests, Biolog identification technology and 16S rDNA sequence analysis; Luo Zhuhua et al. isolated 480 strains of marine bacteria from different sea areas, including 107 strains of faecal Esche...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/22A01N25/04A01P3/00
CPCA01N25/04A01N63/22
Inventor 马桂珍暴增海周丽颖陈茹
Owner JIANGSU OCEAN UNIV
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