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Secondary metabolite of gingko endophytic fungus Nigrospora sphaerica and antibacterial application of secondary metabolite

A technology of secondary metabolites and endophytic fungi, applied in the field of fungal metabolite research, can solve the problem of unscreened active antibacterial substances, and achieve the effect of good inhibitory effect and broad-spectrum antibacterial effect.

Active Publication Date: 2021-03-19
HUNAN UNIV OF SCI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This strain has antibacterial activity against R. solanacearum, but it has not been screened for its active antibacterial substance, which needs to be further studied in detail

Method used

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  • Secondary metabolite of gingko endophytic fungus Nigrospora sphaerica and antibacterial application of secondary metabolite
  • Secondary metabolite of gingko endophytic fungus Nigrospora sphaerica and antibacterial application of secondary metabolite
  • Secondary metabolite of gingko endophytic fungus Nigrospora sphaerica and antibacterial application of secondary metabolite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Separation of Active Secondary Metabolite 9α-Hydroxy Dioxide Deoxy Current

[0034] In the previous work, the inventor was screened to a ginkgo born fungi, which had activity of anti-bluebutum, which was identified as a NiGrospora Sphaerica, named Tea GBH45 (NiGrospora Sphaericagbh45), On November 05, 2018, he was deposited in China's Typical Cultural Warehouse (address for China. Wuhan University), its preservation number is CCTCC NO: M 2018755.

[0035] The GBH45 (NiGrospora Sphaerica GBH45) preserved at -80 ° C was inoculated into the PDA plate, and the growth of the bacteria was grown in the PDA plate (about 3 to 5 days) at 28 ° C, which is the activation of the strain.

[0036] Picking a small amount of mycelium inoculation is located in a 500 ml of PDB liquid medium. In the 500 ml triangular bottle, it is placed in a constant temperature oscillating incubator, and the fermentation culture is 5 ~ 7d under 28 ° C, 200 rpm. It is terminated when the fermentatio...

Embodiment 2

[0050] Example 2 9α-hydroxy dihydrohydrogen deoxidation celocyin on the inhibition of cypros

[0051] Stype activation: Turning -80 ° C inoculated into sterilized Na liquid medium (protein 10.0 g, beef paste powder 3.0g, sodium chloride 5.0g, DD H) 2 O 1L, agar 20.0g, after the configuration was adjusted, pH to 7.4 ± 0.2), 30 ° C, 200 rpm culture overnight.

[0052] Suppression efficiency and MIC and IC 50 Determination of concentration: Take cultivated cyprosy fermentation liquid in the ultra-net workbench to determine its OD 600 Value, and post-sterilized Na medium tone hydrochloride OD 600 Value to 0.1. The target antibacterial active was dissolved with DMSO, and then 2 times diluted with its corresponding culture solution was used to reduce the concentration of the compound to 50 μg / ml, 25 μg / mL, 12.5 μg / mL, 6.25 μg / ml. 3.125 μg / ml (DMSO <1%).

[0053] Medicine blank group OD 600 Determination. Due to the target antibacterial active, light absorption at 600 nm, and t...

Embodiment 3

[0082] Example 3 9 α-hydroxy dihydroxy hydroxybroofer cephalin on the inhibition of U.g. E. coli in common foodgiogenic bacteria

[0083] The inhibitory effect of Typical GBH45 (NiGrospora Sphaerica GBH45) secondary metabolitin was used to study the Oxford Cup method.

[0084] (1) activation of strains

[0085]From the preserved ramps, a small amount of a small amount of a small amount of Escherichia coli body is used, inoculated into sterilized LB liquid medium (protein 10g, yeast extract 5g, sodium chloride 10g, distilled water 1L, pH 7.0-7.2) In 121 ° C sterilization for 20 min, cultured 12h, that is, activation is completed.

[0086] (2) Oxford Cup Filmilory Experiment

[0087] A, 100 μl of Escherichia coli culture solution after activation, 100 times after dilution, taking 100 μL to LB plate, respectively labeled sample, positive control (25 μg / L sulfate), negative control (sterile distilled water) and Blank control (solvent dimethyl sulfoxide) region, placed to the corresp...

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Abstract

The invention discloses a secondary metabolite of gingko endophytic fungus Nigrospora sphaerica. The metabolite is 9 alpha-hydroxyl dihydrodeoxyspiroxin. The 9 alpha-hydroxyl dihydrodeoxyspirotrichinenot only has an inhibiting effect on ralstonia solanacearum, but also has a good inhibiting effect on common typical gram-negative food-borne pathogenic bacteria escherichia coli, typical gram-positive food-borne pathogenic bacteria staphylococcus aureus and bacillus subtilis, the effect of the 9 alpha-hydroxyl dihydrodeoxyspirotrichine is equivalent to that of antibiotic streptomycin sulfate, and the metabolite has important application in the aspects of agricultural biocontrol and food preservation.

Description

Technical field [0001] The present invention belongs to the technical field of fungi metabolites, and in particular, the use of a metabolic product of Ginkgo bilobacteria and its inhibiting gingerbumin, Escherichia coli, Golden Staphylococcus aureus and Bacillus Bacillus. Background technique [0002] Ginkgo is a specific tree species in my country. All of Ginkgo contains a bacterius substance, which has functions such as anti-blindness, cucumber cylpy, and there are many antibacterial ingredients in the extract. There are many inexplicen secretions, but ginkgo. The secondary metabolites of the trifle, therefore, the antibacterial properties of the extracts are different due to the difference in the type and quantity of the endogenous fungus, resulting in different ginkgo extracts, resulting in a different ginkgo tissue. The symbiotic fungi, studied its active secretions, a hot spot in the art. [0003] In the preliminary operation of the present invention, the inventors screened...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/755C12P15/00C12N1/14A01N35/06A01P1/00A23L3/3499C12R1/645
CPCC07C49/755C12P15/00C12N1/14A01N35/06A23L3/34635A23L3/3499C07C2603/24Y02A50/30
Inventor 张祖姣袁志辉何福林刘小文陈婷齐成媚沈玉平
Owner HUNAN UNIV OF SCI & ENG
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