Metal ion dynamic detection and regulation method based on Raman spectrum

A metal ion and Raman spectroscopy technology, applied in the field of image super-resolution realization, can solve the problems of unclear, uncontrollable, insufficient resolution, etc. The effect of good nanoelectrode properties

Inactive Publication Date: 2021-03-19
深圳市新零壹科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The main purpose of the present invention is to solve the problems in the prior art, such as the unclear, uncontrollable, and insufficient resolution of the metal ion detection chelate bond formation process based on Raman spectroscopy, to provide a highly sensitive, high-selective, non-destructive and real-time A method of dynamic detection and regulation of copper ions based on Raman spectroscopy that combines the advantages of detection and other advantages

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  • Metal ion dynamic detection and regulation method based on Raman spectrum
  • Metal ion dynamic detection and regulation method based on Raman spectrum
  • Metal ion dynamic detection and regulation method based on Raman spectrum

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preparation example Construction

[0056] In step 1), the specific method for preparing the gold electrode can be: place a platinum wire coil with a diameter of about 1 cm (the diameter of the platinum wire is 0.1 mm) on the liquid surface of the HCl and ethanol electrolyte with a volume ratio of 1:1, and place A gold wire with a diameter of 0.2mm is placed at the center of the platinum wire circle, and the tip is about 1mm below the liquid surface. The gold wire is connected to the positive pole and the platinum wire circle is connected to the negative pole. The bottom is just in contact with the liquid surface of the electrolyte, and a 4.2KHz 13.5V square wave is applied, and the gold wire is quickly lifted after 0.5 to 1.5 seconds to complete the secondary etching, and a gold nano-electrode with a relatively uniform tip size is obtained.

[0057] 2) Cleaning of gold electrodes;

[0058] In step 2), the specific method of cleaning the gold electrode can be: put the etched end of the etched gold electrode upsi...

Embodiment 1

[0077] Embodiment 1, reference Figure 1 to Figure 5 ,Such as Figure 1 to Figure 5 shown.

[0078] The invention provides a method for dynamic detection and regulation of metal ions based on Raman spectroscopy, comprising the following steps:

[0079] Soak the polytetrafluoroethylene-wrapped gold electrode in a 5mM ethanol solution of p-carboxythiophenol for 7 hours, then wash with ethanol and ultrapure water three times each to remove excess molecules to be tested on the surface of the gold electrode. Add 7 μL 10mM p-carboxythiophenol ethanol solution to 1000 μL 150pM 40nm gold nanosphere solution, incubate at room temperature for 12 hours, centrifuge the resulting solution at 5000r / min to remove the supernatant and wash with ultrapure water, repeat three times to obtain p-carboxythiophenol Phenol-modified 40nm gold nanosphere solution. Clean the electrolytic cell, install the gold nanoelectrodes, and add 90 μL of 5 mM PBS and 3 mM K to the electrolytic cell 4 Fe(CN) 6 ...

Embodiment 2

[0081] Embodiment 2, refer to Figure 6 ,Such as Figure 6 Shown:

[0082] The invention provides a method for dynamic detection and regulation of metal ions based on Raman spectroscopy, comprising the following steps:

[0083] Soak the polytetrafluoroethylene-wrapped gold electrode in a 5mM ethanol solution of p-carboxythiophenol for 7 hours, then wash with ethanol and ultrapure water three times each to remove excess molecules to be tested on the surface of the gold electrode. Add 7 μL 10mM p-carboxythiophenol ethanol solution to 1000 μL 150pM 40nm gold nanosphere solution, incubate at room temperature for 12 hours, centrifuge the resulting solution at 5000r / min to remove the supernatant and wash with ultrapure water, repeat three times to obtain p-carboxythiophenol Phenol-modified 40nm gold nanosphere solution. Clean the electrolytic cell, install the gold nanoelectrodes, and add 90 μL of 5 mM PBS and 3 mM K to the electrolytic cell 4 Fe(CN) 6 Then add 30 μL of p-carbo...

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Abstract

The invention discloses a metal ion dynamic detection and regulation method based on Raman spectroscopy, and relates to a dynamic Raman spectroscopy measurement method. The method comprises the following specific steps of modifying p-carboxyl thiophenol molecules with Raman signals at the tip of a gold electrode and on the surfaces of nanoparticles; establishing a single-molecule Raman signal detection system; when exciting light irradiates a sample, a Raman signal being generated, copper ions being added into the system, and the formation and disappearance of copper ion chelating bonds beingdetected by dynamically detecting the change of a Raman spectrum. The method is advantaged in that a bias voltage on the gold electrode is adjusted, and the change of the intensity of a p-carboxyl thiophenol Raman characteristic peak is detected so that the vibration condition of each bond in the molecular junction is researched; various metal ions such as copper ions and the like can be detectedthrough chelation; the method can be used for researching the strength of the chelating bond effect through voltage regulation, and is wide in application range, high in functionalization, visual in data and simple to operate.

Description

technical field [0001] The invention relates to image super-resolution realization technology, in particular to a method for dynamic detection and regulation of metal ions based on Raman spectroscopy, that is, a new method for detecting copper ions regulated by voltage and its interaction in regulating metal ion chelate bonds. Background technique [0002] At present, Surface Raman Enhanced (SERS) has been proved to be a powerful analytical technique with the advantages of high sensitivity, high selectivity, non-destructive, etc., and does not strictly require the measurement conditions of the sample. At the same time, SERS has also demonstrated the ability to study the internal chemical dynamics of various metal-molecule-metal structures at the single-molecule level, thereby greatly improving our understanding in the fields of metal nanoparticle catalysis, photocatalysis, and molecular electronics. However, current studies mainly focus on the steady-state long-term changes,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 梁峰
Owner 深圳市新零壹科技有限公司
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