A method for making, preserving and identifying individual specimens of non-toxic rainbow trout and perch specimens

A non-toxic technique for making specimens, which is applied in the preservation of human or animal bodies, biochemical equipment and methods, and the determination/inspection of microorganisms. It can solve the problems of specimens losing their scientific research value, achieve light weight, prevent confusion, The effect of long-term use

Active Publication Date: 2022-04-08
中国长江三峡集团有限公司中华鲟研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if there is confusion among individual fish specimens, the specimen loses its scientific value

Method used

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  • A method for making, preserving and identifying individual specimens of non-toxic rainbow trout and perch specimens
  • A method for making, preserving and identifying individual specimens of non-toxic rainbow trout and perch specimens
  • A method for making, preserving and identifying individual specimens of non-toxic rainbow trout and perch specimens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A kind of freshwater rainbow trout and perch (rainbow trout) prepare specimen, and concrete steps are as follows:

[0052] (1) Select a healthy rainbow trout with better body shape and less scale shedding, and suffocate the rainbow trout to death with plastic wrap. Clean the slime off the surface of the fish.

[0053] (2) Take pictures of the appearance of the rainbow trout, and measure the total length, body length, body width, body height, head length, head height, head width, snout length, eye diameter, eye distance, and tail peduncle length of the rainbow trout. The specific data are as follows 1.

[0054] (3) Fix the shape of the rainbow trout head, adjust the opening size of the fish mouth, put the rainbow trout head into the alginate impression, wait for the alginate impression to solidify completely, take the rainbow trout out of the alginate impression, The master model is finished. Make glass glue resin and putty resin mixture. The mixture is slowly poured...

Embodiment 2

[0068] A kind of seawater rainbow trout and perch (perch) prepare specimen, and concrete steps are as follows:

[0069] (1) Choose a healthy perch with better body shape and fewer scales falling off, and suffocate the fish to death with plastic wrap. Clean the slime off the surface of the bass.

[0070] (2) Take pictures of the appearance of the perch, and measure the full length, body length, body width, body height, head length, head height, head width, snout length, eye diameter, eye distance, and tail peduncle length of the sea bass. The specific data are as follows 1.

[0071] (3) Fix the shape of the perch head, adjust the opening size of the perch mouth, put the perch head into the alginate impression, wait for the alginate impression to solidify completely, take the sea bass out of the alginate impression, The master model is finished. Make glass glue resin and putty resin mixture. The mixture is slowly poured into the mother mold, so that the mixture solidifies on...

Embodiment 3

[0085] In the process of making rainbow trout stripping specimens, the muscle DNA of rainbow trout was extracted, and the DNA of the specimen was amplified by PCR with rainbow trout microsatellite primers, and the PCR products were electrophoresed on a 12% polyacrylamide gel. It records the experimental results and saves the genetic information of the specimen.

[0086] After the preparation of the specimen is completed, when there is confusion among multiple rainbow trout specimens, the fin ray DNA of the peeled specimen is extracted, and the microsatellite primers of this species are used for PCR amplification, and the PCR product is condensed in 12% polyacrylamide Run electrophoresis on the gel, record the experimental results on the gel imaging system, compare the obtained genetic information with the genetic information of the previously saved specimens, and confirm the specific individual information of the specimen, so as to realize the individual identification of the r...

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Abstract

The invention relates to a method for making, preserving and identifying individual specimens of non-toxic rainbow trout and perch specimens. The method includes the preparation process, preservation and individual identification of specimens. Fix the fish in a designed motion state and make a fish head model; wrap the entire surface of the specimen with plastic wrap, pour the prepared gypsum into the container, and after the gypsum solidifies, divide the solid gypsum into multiple pieces and take out the fish specimen Put the solid gypsum together, inject foaming material into it, make the shape of the fish specimen, and take out the fish model; choose the side or abdomen of the fish according to the display method to make an incision, and peel off the skin; degrease the skin of the fish, and remove the skin Put it into water dissolved with washing powder, remove the grease on the surface, then degrease with acid clay and sawdust, and air dry; install the fish skin and fish head model on the fish model. The method for preparing specimens of rainbow trout and perch provided by the invention has no physical damage to specimen preparation personnel and visitors.

Description

technical field [0001] The invention belongs to the field of making specimens of rainbow trout and perch, and relates to a method for preparing and preserving non-toxic specimens of rainbow trout and perch, and identifying individual specimens. Background technique [0002] Rainbow trout and perch specimens are of great value in scientific research and teaching demonstrations. The specimens of rainbow trout and perch can let more people understand the appearance characteristics, living habits and growth status of the specimen fish, and can effectively promote the development of the aquaculture industry. At present, the techniques for making specimens of rainbow trout and perch are not yet mature enough, and poisonous chemicals are added to almost all specimen preparation processes of rainbow trout and perch. The earliest specimens were made by adding arsenic trioxide (commonly known as arsenic), which is highly toxic. As the technology of specimen preparation became more a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00C12Q1/6888
CPCA01N1/00C12Q1/6888C12Q2600/156
Inventor 胡亚成
Owner 中国长江三峡集团有限公司中华鲟研究所
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