Graphene transistor label-free DNA sensor and preparation method thereof
A graphene and transistor technology, which is applied in the field of biosensors to achieve the effects of low cost, high sensitivity and reduced operating voltage
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[0034] The present invention also provides the preparation method of the graphene transistor DNA sensor described in the above technical scheme, comprising the following steps:
[0035] (1) Prepare gate, source and drain on the surface of the substrate, so that there is a channel between the source and drain;
[0036] (2) Graphene is tiled on the channel between the source and the drain to obtain a graphene transistor;
[0037] (3) carbon dots are fixed on the grid surface of the graphene transistor obtained in the step (2), to obtain the graphene transistor DNA sensor.
[0038] In the present invention, gate, source and drain are prepared on the substrate surface, so that a channel exists between the source and drain. In the present invention, the preparation of the gate electrode, the source electrode and the drain electrode preferably includes: sequentially vapor-depositing a chromium layer and a gold layer on the surface of the substrate by a thermal evaporation coating m...
Embodiment 1
[0065] Thermal evaporation coating:
[0066] Cut the electronic grade glass into 12×12mm size, ultrasonically clean it with acetone, isopropanol, and ethanol for ten minutes, dry it in an oven, paste the glass piece on a mask plate of a specific shape with high-temperature glue, and weigh an appropriate amount The chromium and gold are put into the tungsten boat to prepare the vacuum thermal evaporation coating.
[0067] When evaporating, first evaporate chromium: the thickness is 8nm.
[0068] Evaporation gold layer again: the thickness is 50nm.
[0069] The obtained electrode shape, structure and size are as follows image 3 shown. image 3Among them, G is the gate, that is, the gate, S is the source, that is, the source, D is the drain, that is, the drain, and the 0.25mm wide channel between the source and the drain is the graphene channel after graphene is transferred.
[0070] Wet transfer of monolayer graphene:
[0071] 250 mg of methyl methacrylate (PMMA) with a mo...
Embodiment 2
[0080] Thermal evaporation coating:
[0081] Cut the electronic grade glass into 12×12mm size, ultrasonically clean it with acetone, isopropanol, and ethanol for ten minutes, dry it in an oven, paste the glass piece on a mask plate of a specific shape with high-temperature glue, and weigh an appropriate amount The chromium and gold are put into the tungsten boat to prepare the vacuum thermal evaporation coating.
[0082] When evaporating, chrome is evaporated first: the thickness is 6nm.
[0083] Evaporate a gold layer again: the thickness is 35nm.
[0084] The shape, structure and size of the obtained electrode are the same as in Example 1.
[0085] Wet transfer of monolayer graphene:
[0086] 250 mg of methyl methacrylate (PMMA) with a molecular weight of 99600 g / mol was dissolved in 5 mL of anisole, and stirred on a magnetic stirrer to obtain a clear and transparent PMMA / anisole solution with a concentration of 50 mg / mL.
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