Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nanowire mediated delivery system and methods comprising the same

A technology of nanowires and nanowire arrays, applied in the field of molecular delivery systems, can solve the problems of long virus transfection process, apoptosis, low delivery efficiency, etc.

Inactive Publication Date: 2021-03-30
COMMONWEALTH SCI & IND RES ORG +1
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current techniques for transfecting live immune cells often yield low delivery efficiencies, activate immune responses, induce nonspecific inflammation, require lengthy viral transfection processes, suffer from biosafety issues, suffer from cargo specificity, and / or require extensive apoptosis. harsh conditions of death

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nanowire mediated delivery system and methods comprising the same
  • Nanowire mediated delivery system and methods comprising the same
  • Nanowire mediated delivery system and methods comprising the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0308] Embodiment 1: Preparation of Si nanowires

[0309] Preparation of Si wafer substrate

[0310] Prior to deposition of polystyrene microspheres (PSMS, Polysciences, Inc.), flat silicon wafers (3″, p-type, 3–6 Ωcm, , Siltronix, France) were cut into quarter pieces and cut at 1 : 1 ethanol: acetone solution ultrasonic cleaning 5min, and then ultrasonic cleaning 5min again in MilliQ water. Then, soak the wafer in boiling piranha solution (3:1H 2 SO 4 :H 2 o 2v / v, 75°C, Avantor Performance Materials) for 1 h to remove any organic contaminants, then washed with water and dried under a nitrogen sparge.

[0311] convective assembly deposition

[0312] A hexagonal close-packed PSMS monolayer was deposited on a quarter-sheet 3″ Si wafer by convection assembly. The setup consisted of a mounted microscope slide used as a PSMS deposition blade, a 50 mm motorized translation stage (MTS50- Z8, Thorlabs, Inc.) and Compact Sub-Hertz Pendulum (CSP) Vibration Isolation System (TM...

Embodiment 2

[0321] Example 2: Preparation of Molecules

[0322] Preparation of fluorescently labeled plasmids

[0323] Using Label IT Tracker TM Intracellular Nucleic Acid Localization Kit (Mirus, Japan) adds fluorescent tags to molecules which are plasmids. Mix 5 µg of gWiz-GFP plasmid with 2.5 µL of Cy3, 5 µL of labeling buffer A (provided in the kit), and 37.5 µL of water to make a final volume of 50 µL. The mixture solution was then incubated at 37°C for 1 h. The Cy3-tagged plasmid was then purified by ethanol precipitation. Figure 13B showed that, for SiNWs, the loading efficiency of these plasmids was 79.4 ± 22.5%, which was comparable to that of the plasmids loaded onto flat Si substrates (81.2 ± 17.8%).

Embodiment 3

[0324] Embodiment 3: the preparation of cell culture

[0325] Four types of cell lines were investigated in this study: GPE86 (ATCC, mouse embryonic fibroblasts), L1.2 (ATCC, mouse B cells), Jurkat (ATCC, human CD4 + T cells), Ramos (ATCC, human B cells), primary human B cells (isolated from human tonsils, approved by the Australian National University Human Research Ethics Committee and University Hospital Institutional Review Board) and primary mouse T cells (isolated from From mouse lymph nodes, approved by the Animal Experimentation Ethics Committee of the Austrian National University). GPE86 cells in 10% fetal bovine serum (FBS, Gibco), 1mM sodium pyruvate, 2mM L-glutamine, 100U mL -1 Penicillin and 100 μg mL -1 Grow and maintain in complete Dulbecco's modified Eagle's medium (DMEM (Gibco)) with streptomycin (Gibco). L1.2, Jurkat and Ramos cells in complete RPMI (RPMI-1640 (Gibco), which includes i0% FBS, 10mM HEPES, 1 × non-essential amino acid solution (Gibco), 1mM s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
lengthaaaaaaaaaa
lengthaaaaaaaaaa
Login to View More

Abstract

The present disclosure relates generally methods of delivering molecules into cells using nanowire (NWs). In particular, the method utilises a molecular delivery system comprising a nanowire (NW) array comprising a plurality of nanowires (NWs) which are used to deliver molecules into cells. The NWs can be functionalised with molecules that can be delivered into cells following contact (e.g. penetration) by the NWs.

Description

technical field [0001] The present invention relates generally to molecular delivery systems and methods of using the molecular delivery systems to deliver molecules into cells. The molecular delivery system includes a nanowire array (NW) including a substrate and a plurality of nanowires (NW). Background technique [0002] Intracellular delivery of bioactive molecules to cells is critical for a number of advanced therapeutic, clinical and research applications. Intracellular delivery of molecules (e.g., nucleic acids) can be achieved using a variety of methods, including viral methods (e.g., adenoviruses, retroviruses, adeno-associated viruses, herpes simplex viruses, and vaccinia viruses), chemical methods (e.g., lipotransfer staining, calcium phosphate, DEA-dextran) and physical methods (eg, electroporation, bombardment, microinjection). [0003] However, there is still a need to develop high-throughput, scalable, efficient, flexible and non-destructive platforms for th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/87B82Y5/00
CPCC12N15/87A61K48/0033A61K48/0041B82Y5/00C12N2535/00C12M23/12A61K39/4611A61K39/464412A61K39/4631C12M35/00C12M35/04
Inventor N·H·沃尔克R·埃尔纳森S·阿斯拉诺格鲁Y·陈
Owner COMMONWEALTH SCI & IND RES ORG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com