A kind of soybean detoxification method and detoxified soybean
A soybean and seed technology, applied in food science and other directions, can solve the problems of not effectively improving soybean bean smell and reducing harmful components, and achieve the effects of reducing toxicity, improving utilization rate and improving taste.
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Embodiment 1
[0033] Embodiment 1 A kind of method of soybean detoxification
[0034] Specifically include the following steps:
[0035] S1. After the soybeans are washed and dried, the surface is polished;
[0036] S2. Dissolve 12g of sucrose, 3g of alanine, 0.5g of vitamin E and 0.1g of zinc sulfate in 100mL of sterile water, mix well, adjust the pH value of the culture medium to 6.5 with PBS solution, sterilize with ultraviolet light for later use, and obtain a nutrient solution ;
[0037] S3. Inoculate 10g of Bifidobacterium, 2g of Lactobacillus bulgaricus, 1g of Candida utilis, and 2g of Bacillus licheniformis into Gao's culture medium for marking, anaerobic culture, the anaerobic culture condition is 27 ℃, and the humidity is 75%, cultivated for 1 day, and then cultivated into the seed liquid of the strains respectively, with a bacterial content of 10 8 cfu / mL;
[0038] S4. the strain seed liquid that step S3 is made is inoculated respectively in the nutrient solution that step S2...
Embodiment 2
[0045] Embodiment 2 A kind of method of soybean detoxification
[0046] Specifically include the following steps:
[0047] S1. After the soybeans are washed and dried, the surface is polished;
[0048] S2. Dissolve 20g of maltose, 3g of threonine, 4g of tryptophan, 1g of vitamin B12 and 0.5g of calcium chloride in 100mL of sterile water, mix well, adjust the pH value of the medium to 7.5 with PBS solution, and sterilize with ultraviolet light Standby to obtain nutrient solution;
[0049] S3. Inoculate 10g of Bifidobacterium, 5g of Lactobacillus bulgaricus, 3g of Candida utilis, and 7g of Bacillus licheniformis into Gao's culture medium, respectively, for anaerobic culture. The anaerobic culture conditions are 35°C and the humidity is 85%, cultivated for 2 days, and then cultivated into the seed liquid of the strain respectively, and the bacterial content was 10 9 cfu / mL;
[0050] S4. the strain seed liquid that step S3 is made is inoculated respectively in the nutrient sol...
Embodiment 3
[0057] Embodiment 3 A kind of method of soybean detoxification
[0058] Specifically include the following steps:
[0059] S1. After the soybeans are washed and dried, the surface is polished;
[0060] S2. Dissolve 16g molasses (molasses is sugarcane molasses and beet molasses, mass ratio is 1:3), 5g urea, 0.3g vitamin A, 0.4g vitamin B1 and 0.3g magnesium sulfate with 100mL sterile water, after mixing evenly, Use PBS solution to adjust the pH value of the culture medium to 7, sterilize with ultraviolet light for standby, and obtain a nutrient solution;
[0061] S3. Inoculate 10g of Bifidobacterium, 3.5g of Lactobacillus bulgaricus, 2g of Candida utilis, and 5g of Bacillus licheniformis into Gao's culture medium, respectively, for anaerobic culture. The anaerobic culture conditions are 30°C and humidity 80%, cultivated for 1.5 days, and then cultivated into the seed liquid of the strain respectively, and the bacterial content was 10 8 cfu / mL;
[0062] S4. the strain seed l...
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