Production method of glucosamine

A technology of glucosamine and production method, which is applied in the field of glucosamine production, can solve the problems of low glucosamine yield and conversion rate, and achieve the effect of meeting the growth requirements of bacteria and high yield and conversion rate

Pending Publication Date: 2021-04-02
INNER MONGOLIA KINGDOMWAY PHARMA LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of low yield and conversion rate in the production of gluc

Method used

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  • Production method of glucosamine
  • Production method of glucosamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1: 100L tank fermentation production process

[0062] (1) Seed activation

[0063] Draw a little bacterial solution from the seed preservation tube for gradient dilution, draw a little bacterial suspension onto the plate medium, and cultivate at 32°C for 24 hours to obtain a mature single colony. The plate medium components are as follows: sodium chloride 5g / L, peptone 10g / L, yeast extract powder 10g / L, agar powder 20g / L, adjust its pH value to 7.5 before sterilization, and then in 121 ℃ Sterilize for 25 minutes.

[0064] (2) shake flask culture

[0065]Pick 3 single colonies from the mature plate culture medium into a shake flask filled with 100mL shake flask culture medium. The shake flask is a 1L Erlenmeyer flask and cultured on a shaker at a culture temperature of 32°C and a rotation speed of 220rpm for 20h When the wet weight of the thalline reaches 8g / L, it can be moved to the seed tank for cultivation. The shake flask medium components are as follo...

Embodiment 2

[0077] Example 2: 5m 3 Tank fermentation production process

[0078] (1)-(3) are the same as embodiment 1;

[0079] (4) Fermentation culture

[0080] Prepare the fermentation medium and sterilize it at 121°C for 25 minutes, adjust its pH value to 7.2 before sterilization, and move the seed liquid of the seed tank to 5m according to the inoculation amount of 15%. 3 Fermentation tank, liquid volume is 2.5m 3 . Initial culture conditions: culture temperature 32°C, rotation speed 150rpm, aeration ratio 0.5VVM, tank pressure 0.03MPa, the pH value is controlled at around 7.2 by supplementing ammonia water during the fermentation culture process, and the feeding concentration during the fermentation culture process is 55% glucose solution and 0.4% sulfuric acid Ammonium is used to control the content of carbon source and nitrogen source at 0.5g / L and 1.1g / L respectively.

[0081] The fermentation medium components are glucose 10g / L, sodium dihydrogen phosphate 10g / L, magnesium c...

Embodiment 1-1

[0091] Example 1-1: 5m 3 Tank fermentation production process

[0092] (1)-(3) are the same as embodiment 1;

[0093] (4) Fermentation culture

[0094] Prepare the fermentation medium and sterilize it at 121°C for 25 minutes, adjust its pH value to 7.2 before sterilization, and move the seed liquid of the seed tank to 5m according to the inoculation amount of 15%. 3 Fermentation tank, liquid volume is 2.5m 3 . Initial culture conditions: culture temperature 32°C, rotation speed 150rpm, aeration ratio 0.5VVM, tank pressure 0.03MPa, the pH value is controlled at around 7.2 by supplementing ammonia water during the fermentation culture process, and the feeding concentration during the fermentation culture process is 55% glucose solution and 0.4% sulfuric acid Ammonium is used to control the content of carbon source and nitrogen source at 0.5g / L and 1.1g / L respectively.

[0095] The fermentation medium components are glucose 10g / L, sodium dihydrogen phosphate 10g / L, magnesium...

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Abstract

The invention belongs to the field of glucosamine production, and relates to a production method of glucosamine. The production method comprises the steps of: in the glucosamine fermentation process,monitoring at least one of an oxygen consumption rate, an oxidation-reduction potential, acetic acid concentration and a specific carbon dioxide release rate in fermentation liquor on line, and controlling the numerical value within a specific range by stages. According to the method provided by the invention, at least one of the oxygen consumption rate, the oxidation-reduction potential, the acetic acid concentration and the specific carbon dioxide release rate in the fermentation liquor is monitored and controlled on line to feed back process parameters of the regulation and control process,so that the thallus growth requirement can be met, glucosamine metabolic synthesis is effectively promoted, and the conversion rate is increased; and the content and conversion rate of the glucosamine in the obtained fermentation product are both high.

Description

technical field [0001] The invention belongs to the field of glucosamine production, in particular to a production method of glucosamine. Background technique [0002] Glucosamine is the constituent unit of various polysaccharides in organisms, and has a wide range of applications. It is clinically used as a drug for the treatment of rheumatism and rheumatoid arthritis. It can be used as a food antioxidant, food additive for infants, and sweetener for diabetics. It can also be used clinically to enhance the function of the human immune system, inhibit the excessive growth of cancer cells or fibroblasts, inhibit and treat cancer and malignant tumors, and effectively treat various inflammations. Pain is also therapeutic. [0003] At present, the methods for producing glucosamine mainly include acid hydrolysis, enzymatic hydrolysis and microbial fermentation. Among them, the production of glucosamine by acid hydrolysis and enzymatic hydrolysis will have adverse effects on the...

Claims

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Application Information

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IPC IPC(8): C12P19/26C12P39/00C12N1/20C12N1/18C12N1/14C12R1/125C12R1/865C12R1/845C12R1/66C12R1/785C12R1/19
CPCC12P19/26C12P39/00C12N1/20C12N1/18C12N1/14
Inventor 朱志春陈必钦严必能李丹胡丽娜刘子睿
Owner INNER MONGOLIA KINGDOMWAY PHARMA LTD
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