Composition comprising stem cell-derived exosome containing culture as effective ingredient for preventing or treating arthritis

A technology for active ingredients and arthritis, which can be used in medical preparations containing active ingredients, drug combinations, medical raw materials derived from mammals, etc., and can solve the problems of low concentration and low treatment effect of arthritis.

Pending Publication Date: 2021-04-02
设计细胞股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the concentration of functional components such as cartilage regeneration promoting factors in the stem cell culture medium is too low, and there is a problem that the actual treatment effect of arthritis is lower than the direct transplantation of stem cells only by the administration of the stem cell culture medium.

Method used

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  • Composition comprising stem cell-derived exosome containing culture as effective ingredient for preventing or treating arthritis
  • Composition comprising stem cell-derived exosome containing culture as effective ingredient for preventing or treating arthritis
  • Composition comprising stem cell-derived exosome containing culture as effective ingredient for preventing or treating arthritis

Examples

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specific Embodiment approach

[0050] Hereinafter, the present invention will be further described in detail through examples. These examples are only to illustrate the present invention more specifically, and the scope of the present invention is not limited by these examples.

[0051] Preparation Example: Stem Cells

[0052] In the following examples, the following cells were used for stem cells in order to obtain a stem cell culture medium rich in active ingredient exosomes for confirming the therapeutic effect of arthritis.

[0053] First of all, immortalized amniotic fluid stem cells (AFSC), immortalized neural stem cells (NSC) and immortalized oligodendrocyte precursor cells (OPC) are cell lines established by the inventors of the present invention as immortalized cell lines, and each cell The strains have obtained the deposit number from the Microorganism Depository Agency, and the immortalized neural stem cell CBNU-NSC cell line (preservation number: KCTC12635BP, Korea Authorized Patent No. 10-171927...

Embodiment 1

[0055] 1-1 Stem cell culture and acquisition of exosome-rich culture medium

[0056] The prepared stem cells (1x 10 6 Cells / mL) were inoculated in Dulbecco's Modified Eagle's Medium (DMEM) in 5% carbon dioxide (CO 2 ) in the supply medium for 24 hours at 37°C. Afterwards, in order to obtain exosome-enriched medium (ERCM) from stem cells, TNF-α was added to the above medium at a concentration of 50 ng / mL, and the oxygen concentration was reduced to 5%. After culturing for 24 hours, the culture medium was collected and filtered. filter, and then concentrated 10 times at low temperature to obtain the culture solution rich in exosomes derived from stem cells according to the present invention.

[0057] As a comparative example, in the process of obtaining exosome-rich culture fluid from stem cells respectively, in the process of Example 1, except for TNF-α (50ng / mL) treatment and oxygen (O 2 ) concentration was maintained at 21%, the same method as in Example 1 was carried ou...

experiment example 1

[0066] Analysis of the therapeutic effect of the present invention's stem cell-rich exosome culture fluid on arthritis

[0067] 1-1 Breeding of Rabbits for Experimental Animals

[0068] Eight-month-old (3.5kg) male New Zealand white rabbits (New Zealand white, NZW) were used after 2 weeks of laboratory acclimatization. One experimental animal was accommodated in each rabbit cage, and the environment of the animal laboratory was adjusted to a temperature of 23±2°C, a relative humidity of 55±10%, a ventilation rate of 12 times / hour, and a lighting cycle of 12 hours (07:00- 19:00), the illuminance was 150-300lux for feeding. The feed is freely ingested as a small particle type (pallet) solid feed Purina Rabbit for experimental animals and sterilized purified water. This experiment was approved by the Institutional Animal Care and Use Committee (IACUC) of the Experimental Animal Research Support Center of Chungbuk University, in accordance with the standard operation guidel...

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Abstract

The present invention relates to a composition of a stem cell-derived exosome-containing culture for preventing or treating arthritis and, more particularly, to a pharmaceutical composition comprisinga stem cell-derived exosome-containing culture as an effective ingredient for preventing or treating arthritis, wherein the stem cell-derived exosome-containing culture is obtained by treating a stemcell culture with TNF-[alpha] and incubating the stem cell culture in the condition of a 1-8 % oxygen concentration. Containing a large amount of exosomes containing functional effective ingredientssuch as a cartilage regeneration promoting factor, etc., the stem cell-derived exosome-containing culture according to the present invention was found to have a far higher therapeutic effect on arthritis, compared to conventional stem cell transplantation methods for treatment of arthritis and the application of cultures obtained in general stem cell culturing conditions. Hence, the stem cell-derived exosome-containing culture according to the present invention has the effect of being available as a new therapeutic agent for effective treatment of arthritis.

Description

technical field [0001] The present invention relates to a composition for preventing or treating arthritis comprising a culture solution containing exosomes secreted from stem cells as an active ingredient. Background technique [0002] Arthritis is one of the most common diseases causing pain, stiffness, and motor insufficiency. Rheumatoid arthritis (RA) is an inflammatory disease caused by an autoimmune response to type II collagen (collagen II), accompanied by joint edema and pain. In contrast, osteoarthritis (OA) is characterized by the degeneration of joints, which induces pain and joint deformation caused by the destruction of chondrocytes and tissues. Especially with the aging and apoptosis of osteoarthritis, the decrease in the number and function of chondrocytes accelerates the growth of peptidoglycan (peptidoglycans, PG), type II collagen (collagen II) and polysaccharides. Proteoglycan (aggrecan) and other normal extracellular matrix (extracellular matrix, ECM) c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/30A61K35/28A61K35/50A61K9/127A61P19/02A61P29/00A61P21/00A61P19/06A61P37/02
CPCA61K9/127A61K35/28A61K35/30A61K35/50
Inventor 姜明姬崔恩京徐妲韵
Owner 设计细胞股份有限公司
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