Method for rapidly detecting zilpaterol residues in pork

A technology for quantitative detection of pork, applied in measurement devices, preparation of test samples, instruments, etc., can solve problems such as difficulty in meeting rapid sample screening requirements, expensive instruments and equipment, and complicated operations, and achieve fast and convenient detection methods. good accuracy and precision

Active Publication Date: 2021-04-09
JIANGNAN UNIV +1
View PDF20 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromatography and mass spectrometry generally have defects such as long pretreatment process, complicated and technical operation, and exp

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly detecting zilpaterol residues in pork
  • Method for rapidly detecting zilpaterol residues in pork
  • Method for rapidly detecting zilpaterol residues in pork

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0066]Example 1

[0067]A method of quantitatively detecting pork in pork, including the following steps:

[0068](1) Preparation of the Raman enhancement substrate:

[0069]Using the glassware (round bottom flask) used by Water Soil and the rotor for 12h, remove the water to the taste, then rinse three times with ultrapure water, dried 60 ° C to dry, spare; formulated mass fraction is 1% sodium citrate Aqueous solution, 10 mg / ml of potassium potassium potassium titrates, spare; heat up the oil bath cope to 120 ° C and remain constant; set magnetic agitator parameters: 565R / min;

[0070]47 ml of ultra-pure water was added to a round bottom flask and a thorough mixing; a round bottom flask was placed in a 120 ° C oil bath, while stirring with a magnetic stirrer and kept constant to a solution to boiling; add 2 ml 1 % The aqueous solution of citrate continues to stir at 120 ° C for 20 min; cool to normal temperature to obtain a gold solution, spare.

[0071](2) Standards Qiptro's qualitative pea...

Example Embodiment

[0087]Example 2 Extraction of the solvent optimization

[0088]Adjusting the extraction solvent ethyl acetate in the pork extract in Example 1 was acetonitrile or isopropyl alcohol, other and Example 1 was consistent, and the detection was performed.

[0089]The test results are: When the extraction solvent is acetonitrile or isopropyl alcohol, the characteristic peak of Qip Patro has not appeared, and the extraction of ethyl acetate is better.

Example Embodiment

[0090]Example 3 Optimization of detection ratio

[0091]Adjusting the volume ratio of the bidder and the gold sol number ratio in Example 1, the volume ratio of the Qip Patro pork is 1: 1 or 3: 1, other and Example 1, which is consistent. .

[0092]The test results are: selected 1113cm-1Raman Peak at office is referenced, and the gold sol with the liquid solution is mixed according to different ratios.Figure 6As shown, the gold sol with the fluid volume of 1: 2 is 1: 2, the signal strength is the highest.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for rapidly detecting zilpaterol residues in pork, which belongs to the field of food detection. A Raman characteristic peak of zilpaterol is determined by using a density functional theory and a zilpaterol standard solution; a quantitative analysis curve Y=5214.7X-35.844 by using a characteristic peak 1113cm<-1> with strong Raman characteristic peak signal and good peak shape, and the correlation coefficient R<2> is 0.9355; then the pork sample is pretreated, and extracting is conducted by adopting an extraction solvent to obtain a to-be-detected solution; and testing is conducted to obtain the concentration of zilpaterol in the to-be-detected sample according to the Raman spectrogram of the to-be-detected solution and the standard curve. The lowest detection concentration of zilpaterol in pork detected by the method is 5mg/L, zilpaterol residues in pork can be qualitatively and quantitatively analyzed, and the detection time of a single sample is controlled within 3min.

Description

technical field [0001] The invention relates to a rapid detection method for zilpaterol residues in pork, belonging to the field of food detection. Background technique [0002] Veterinary drug residues refer to the parent compound and / or its metabolites of veterinary drugs contained in any edible part of animal products, as well as impurities related to veterinary drugs. [0003] Veterinary drugs and feed additives remaining in animal-derived food enter the human body along the food chain, posing a potential threat to human health. This threat has attracted more and more attention. With the change of people's demand for animal-derived food, the international requirements for animal residues in animal-derived food are getting higher and higher, and veterinary drug residues in animal-derived food have gradually become a focus of attention all over the world. Zipaterol is a β-receptor agonist, commonly known as "clenbuterol". The application of food animals originated in the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/65G01N1/28
CPCG01N21/658G01N1/28
Inventor 谢云飞董祥辉施思倩姚卫蓉孙莹莹邱险辉
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap