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Method for rapidly detecting zilpaterol residues in pork

A technology for quantitative detection of pork, applied in measurement devices, preparation of test samples, instruments, etc., can solve problems such as difficulty in meeting rapid sample screening requirements, expensive instruments and equipment, and complicated operations, and achieve fast and convenient detection methods. good accuracy and precision

Active Publication Date: 2021-04-09
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromatography and mass spectrometry generally have defects such as long pretreatment process, complicated and technical operation, and expensive instruments and equipment, which make it difficult to meet the requirements for rapid screening of a large number of samples on site

Method used

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  • Method for rapidly detecting zilpaterol residues in pork
  • Method for rapidly detecting zilpaterol residues in pork
  • Method for rapidly detecting zilpaterol residues in pork

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] A method for quantitatively detecting zilpaterol in pork, comprising the steps of:

[0068] (1) Preparation of Raman enhanced substrate:

[0069] Soak the used glassware (round bottom flask) and rotor with aqua regia for 12 hours, take it out and wash it with water until it is odorless, then rinse it with ultrapure water three times, dry it in an oven at 60°C, and set it aside; the prepared mass fraction is 1% trisodium citrate Aqueous solution, 10mg / mL potassium chloroaurate solution, set aside; raise the temperature of the oil bath to 120°C and keep it constant; set the magnetic stirrer parameters: 565r / min;

[0070] Add 47mL of ultrapure water and 3mL of potassium chloroaurate into the round bottom flask and mix thoroughly; put the round bottom flask into a 120°C oil bath while stirring with a magnetic stirrer and keep the temperature constant until the solution boils; add 2mL of 1 % Trisodium citrate aqueous solution continued to stir at 120°C for 20 minutes; coole...

Embodiment 2

[0087] The optimization of embodiment 2 extraction solvent

[0088] Adjust the extraction solvent ethyl acetate in the pork extract in Example 1 to be acetonitrile or isopropanol, and keep the others consistent with Example 1 for detection.

[0089] The test results are: when it is found that the extraction solvent is acetonitrile or isopropanol, the characteristic peaks of zilpaterol do not appear, and the extraction effect of ethyl acetate is better.

Embodiment 3

[0090] The optimization of embodiment 3 detection ratio

[0091] Adjust the volume ratio of the standard curve simulation solution and the gold sol in Example 1, the volume ratio of the zilpaterol pork standard extract and the gold sol to 1:1 or 3:1, and keep the others consistent with Example 1 for detection .

[0092] The test result is: selected 1113cm -1 The Raman peak at is used as a reference, and the gold sol is mixed with the test solution according to different proportions, and the results are as follows Figure 6 As shown, the signal intensity is the highest when the volume of gold sol and the solution to be tested is 1:2.

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Abstract

The invention discloses a method for rapidly detecting zilpaterol residues in pork, which belongs to the field of food detection. A Raman characteristic peak of zilpaterol is determined by using a density functional theory and a zilpaterol standard solution; a quantitative analysis curve Y=5214.7X-35.844 by using a characteristic peak 1113cm<-1> with strong Raman characteristic peak signal and good peak shape, and the correlation coefficient R<2> is 0.9355; then the pork sample is pretreated, and extracting is conducted by adopting an extraction solvent to obtain a to-be-detected solution; and testing is conducted to obtain the concentration of zilpaterol in the to-be-detected sample according to the Raman spectrogram of the to-be-detected solution and the standard curve. The lowest detection concentration of zilpaterol in pork detected by the method is 5mg / L, zilpaterol residues in pork can be qualitatively and quantitatively analyzed, and the detection time of a single sample is controlled within 3min.

Description

technical field [0001] The invention relates to a rapid detection method for zilpaterol residues in pork, belonging to the field of food detection. Background technique [0002] Veterinary drug residues refer to the parent compound and / or its metabolites of veterinary drugs contained in any edible part of animal products, as well as impurities related to veterinary drugs. [0003] Veterinary drugs and feed additives remaining in animal-derived food enter the human body along the food chain, posing a potential threat to human health. This threat has attracted more and more attention. With the change of people's demand for animal-derived food, the international requirements for animal residues in animal-derived food are getting higher and higher, and veterinary drug residues in animal-derived food have gradually become a focus of attention all over the world. Zipaterol is a β-receptor agonist, commonly known as "clenbuterol". The application of food animals originated in the ...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/28
CPCG01N21/658G01N1/28
Inventor 谢云飞董祥辉施思倩姚卫蓉孙莹莹邱险辉
Owner JIANGNAN UNIV
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