RNA bacteriostatic agent siR2 and crop pathogen inhibitor
A technology of inhibitors and bacteriostatic agents, which can be used in DNA/RNA fragments, fungicides, recombinant DNA technology, etc. good bacteria effect
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[0018] The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:
[0019] 1. Experiment preparation
[0020] (1) Discovery of tomato siR2
[0021] Using sRNAomics and real-time quantitative RT-PCR verification, a tomato endogenous small RNA that can target the coding gene of Botrytis cinerea was found, which has no homology with all tomato and even all plant miRNAs reported so far , and does not have the stem-loop precursor structure characteristic of plant miRNA, so we define it as siRNA and name it siRNA—siR2.
[0022] (2) Synthesis of siR2 drug
[0023] The siR2 drug was synthesized at Gemma Genetics (Shanghai, China). Add 1OD of siR2 drug to each tube, add RNase-free ddH according to the instructions 2 O, the final concentration of siR2 drug was 10 μM.
[0024] (3) Cultivation of Botrytis cinerea spores
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Abstract
Description
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