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Pharmaceutical composition for treating oral diseases

A composition and drug technology, applied in the field of oral microorganisms, can solve problems such as limited effects, and achieve the effect of inhibiting the formation of Streptococcus mutans biofilm

Active Publication Date: 2021-04-16
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the effect of relying on direct sterilization of sodium fluoride to prevent dental caries is limited

Method used

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  • Pharmaceutical composition for treating oral diseases
  • Pharmaceutical composition for treating oral diseases
  • Pharmaceutical composition for treating oral diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Effect of embodiment 1 biofilm inhibitor on the normal growth of Streptococcus mutans

[0025] Pick the resuscitated Streptococcus mutans and inoculate it in BHI liquid medium for 24 hours, then use BHI medium containing 1% sucrose to adjust the concentration of the bacterial solution to 2.0×10 6 CFU / mL. Add N-(3-cyclobutyrolactone)-4-bromophenylbutyramide with final concentrations of 10, 50, 100 and 200 μM to the Streptococcus mutans bacteria liquid, and add it to a 96-well plate, 150 μL / well, so as not to The bacterial solution containing biofilm inhibitor (containing DMSO with a final concentration of 0.1%) was used as a control, placed in a constant temperature incubator at 37°C for 24h, and the optical density (OD) value at 600nm was detected with a microplate reader. Make 6 parallel holes in groups.

[0026] From figure 1 It can be seen that compared with the control group, the compound has no effect on the normal growth of Streptococcus mutans in the concentra...

Embodiment 2

[0027] The influence of embodiment 2 biofilm inhibitors on Streptococcus mutans biofilm formation

[0028] After cultivating Streptococcus mutans according to the method described in Example 1, suck off the bacterial solution in the 96-well plate, add 280 μL sterile water to each well and wash twice to remove free bacteria, and then clean the wells with clean filter paper. The remaining liquid was blotted dry, and 150 μL of 1% crystal violet solution was added to each well, and stained for 15 minutes. After staining, suck off the crystal violet solution in the well, add 280 μL of sterile water to wash 3 times, and dry the remaining liquid in the well with clean filter paper. Add 150 μL of 33% acetic acid solution to each well to dissolve the biofilm-crystal violet complex, then use a microplate reader to detect the OD value at 570 nm, and make 6 parallel wells for each group.

[0029] The data were expressed as mean±SD, and the comparison between groups was performed by one-w...

Embodiment 3

[0034] Embodiment 3 Composition of the present invention is to the influence of streptococcus mutans normal growth

[0035] Streptococcus mutans was inoculated in BHI liquid medium and cultured for 24 hours, and the concentration of the bacterial solution was adjusted to 2.0×10 with BHI medium containing 1% sucrose. 6CFU / mL. Add NaF solutions with a final concentration of 0.10%, 0.05% and 0.025% to the Streptococcus mutans bacteria solution respectively, add them to a 96-well plate, 200 μL / well, use the bacteria solution without NaF solution as a control, and place it in a 37°C Cultivate in a constant temperature incubator for 24 hours, check the OD value at 600nm with a microplate reader, make 3 parallel wells for each group, and measure the effects of different concentrations of NaF on the growth of Streptococcus mutans.

[0036] The biofilm inhibitor N-(3-cyclobutyrolactone)-4-bromophenylbutyramide was combined with NaF for Streptococcus mutans, wherein the concentration o...

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Abstract

Streptococcus mutans is the most important pathogenic bacteria causing dental caries. When streptococcus mutans is adhered to the surfaces of teeth in a biofilm form, a dental plaque biofilm can be formed. Due to the film forming effect of dental plaque microorganisms, dental plaque bacteria generate drug resistance to antibiotics or bactericides, and the effect of preventing and treating decayed teeth and periodontal diseases is reduced. The invention discloses a composition capable of inhibiting streptococcus mutans biofilm formation. According to the composition, a biological membrane inhibitor and sodium fluoride are combined for use, and formation of a biological membrane is reduced by interfering quorum sensing, so that bacterial drug resistance is reduced, and the efficiency of sodium fluoride is improved.

Description

technical field [0001] The invention belongs to the technical field of oral microorganisms, and in particular relates to the joint application of acylated homoserine lactone compounds and sodium fluoride to inhibit the formation of Streptococcus mutans biofilm, and its role in the prevention and treatment of dental caries, periodontitis and periimplantitis. application in disease. Background technique [0002] Dental caries is a disease in which the hard tissue of the tooth body is chronically and progressively destroyed under the influence of various factors mainly including bacteria. According to the fourth national oral epidemiological survey in 2015, the caries rate of deciduous teeth of 5-year-old children was 70.9%, that of 12-year-old children was 34.5%, and that of middle-aged people aged 35 to 44 was 89.0%. , 98.0% of the elderly aged 65 to 74 suffer from dental caries, and the prevalence of dental caries is on the rise. Dental caries is listed by WHO as one of th...

Claims

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Application Information

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IPC IPC(8): A61K31/365A61K33/16A61P31/04A61P1/02
Inventor 李洋顾艳李红玉
Owner LANZHOU UNIVERSITY
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