A bifunctional monomer double imprinted layer testosterone molecularly imprinted magnetic nanosphere and its preparation method and application

A technology of bifunctional monomers and magnetic nanospheres, applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., can solve the problem of low adsorption capacity of testosterone molecularly imprinted polymers, achieve good regeneration ability, fast Adsorption rate, effect of enhanced interaction

Active Publication Date: 2022-03-08
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to overcome the shortcomings of the above-mentioned prior art, the object of the present invention is to provide a bifunctional monomer double-imprinted layer testosterone molecularly imprinted magnetic nanosphere and its preparation method and application, so as to solve the problem of low adsorption capacity of testosterone molecularly imprinted polymers, Adsorption and removal of testosterone at the cellular level

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  • A bifunctional monomer double imprinted layer testosterone molecularly imprinted magnetic nanosphere and its preparation method and application
  • A bifunctional monomer double imprinted layer testosterone molecularly imprinted magnetic nanosphere and its preparation method and application
  • A bifunctional monomer double imprinted layer testosterone molecularly imprinted magnetic nanosphere and its preparation method and application

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Embodiment 1

[0032] The preparation method of the bifunctional monomer double imprinted layer testosterone molecularly imprinted magnetic nanosphere comprises the following steps:

[0033] Step 1. Put 100mg of magnetic nanoparticles modified by amphiphilic functional groups, 15mg of testosterone, and 30mL of alcohol-water mixture (1:9) in a reaction vessel to react for 30 minutes to form a carrier-template complex; then add 3mL of ammonia water, 100 μL of 3-aminopropyltriethoxysilane, 100 μL of phenyltrimethoxysilane and 2 mL of ethyl orthosilicate were reacted for 6 hours. After the reaction, the reaction product was washed with ultrapure water until it was neutral, and then heated at 30°C, 0.05 Dry at MPa for 3h;

[0034]Step 2: Elute the dried solid polymer in step 1 with a mixture of absolute ethanol and acetic acid at a volume ratio of 90:10, and wash the eluted solid polymer with ultrapure water until neutral. Then, the obtained product was dried at 30° C. and 0.05 MPa for 3 h to ob...

Embodiment 2

[0046] The preparation method of the bifunctional monomer double imprinted layer testosterone molecularly imprinted magnetic nanosphere comprises the following steps:

[0047] Step 1. Put 200mg of magnetic nanoparticles modified by amphiphilic functional groups, 25mg of testosterone, and 40mL of alcohol-water mixture (1:9) in a reaction vessel to react for 40min to form a carrier-template complex; then add 3.5mL of ammonia water , 150 μL of 3-aminopropyltriethoxysilane, 150 μL of phenyltrimethoxysilane and 2.5 mL of ethyl orthosilicate were reacted for 7 hours. After the reaction, the reaction product was washed with ultrapure water to neutrality, and then heated at 30 ° C , Dry at 0.05MPa for 3h;

[0048] Step 2: Elute the dried solid polymer in step 1 with a mixture of absolute ethanol and acetic acid with a volume ratio of 91:9, and wash the eluted solid polymer with ultrapure water until neutral. Then, the obtained product was dried at 30° C. and 0.05 MPa for 3 hours to o...

Embodiment 3

[0059] The preparation method of the bifunctional monomer double imprinted layer testosterone molecularly imprinted magnetic nanosphere comprises the following steps:

[0060] Step 1. Put 300mg of magnetic nanoparticles modified by amphiphilic functional groups, 35mg of testosterone, and 60mL of alcohol-water mixture (2:8) in a reaction vessel for 60 minutes to form a carrier-template complex; then add 4mL of ammonia water, 200 μL of 3-aminopropyltriethoxysilane, 200 μL of phenyltrimethoxysilane and 3 mL of ethyl orthosilicate were reacted for 8 hours. After the reaction, the reaction product was washed with ultrapure water to neutrality, and then heated at 30 ° C, 0.05 Dry at MPa for 3h;

[0061] Step 2: Elute the dried solid polymer in step 1 with a mixture of absolute ethanol and acetic acid with a volume ratio of 92:8, and wash the eluted solid polymer with ultrapure water until neutral. Then, the obtained product was dried at 30° C. and 0.05 MPa for 3 hours to obtain the...

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Abstract

The invention discloses a bifunctional monomer double imprinted layer testosterone molecularly imprinted magnetic nanosphere and a preparation method and application thereof, which belong to the technical field of nanomaterial preparation and nanomedicine. Using magnetic nanospheres modified with amphiphilic functional groups as the carrier, testosterone as the template molecule, and 3-aminopropyltriethoxysilane and phenyltrimethoxysilane as the bifunctional monomers, the template and surface were immobilized in two steps. The bifunctional monomer double imprinted layer testosterone molecularly imprinted magnetic nanospheres were prepared by combining imprinting. The bifunctional monomer double imprinted layer testosterone molecularly imprinted magnetic nanosphere prepared by the method of the present invention has uniform particle size, good structural stability, high selectivity, rich functional monomer groups, and good biological safety; the method is a A new method for preparing molecularly imprinted polymers with high adsorption capacity and high selectivity for testosterone can significantly improve the efficiency of castration therapy for prostate cancer by fully adsorbing and removing intracellular testosterone, and provide new ideas for endocrine therapy for prostate cancer.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation and nanomedicine, and relates to a bifunctional monomer double imprinted layer testosterone molecularly imprinted magnetic nanosphere and a preparation method and application thereof. Background technique [0002] Prostate cancer is one of the common malignant tumors of the male urinary system. The incidence of prostate cancer in my country has shown an obvious and continuous growth trend. Prostate cancer is becoming a malignant tumor of the urinary system that seriously affects the health of men in my country. The prostate is an androgen-dependent organ, and its growth and metastasis are closely dependent on androgen. After the androgen is secreted, it enters the cell, binds to the androgen receptor, and then enters the nucleus to initiate gene expression, thereby promoting the proliferation and metastasis of prostate cancer. The mechanism of action of the clinically applied meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G77/26C08J9/26B01J20/26B01J20/28B01J20/30B01D15/00C08L83/08
Inventor 唐骁爽宋慧佳张海频豆晓杰张博文范瑞韬高瑞霞
Owner XI AN JIAOTONG UNIV
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