Combined detection method of flow cytometry in humoral cellular immunoassay

Abstract

The invention provides a combined detection method of flow cytometry in humoral cellular immunoassay, and relates to the technical field of immunoassay. The combined detection method of the flow cytometry in humoral cellular immunoassay comprises the following steps of: S1, collecting a humoral specimen; S2, evaluating the quality and quantity of specimens; S3, carrying out conventional cell population classification, multi-label immune typing, cytokine analysis and tumor marker inspection; and S4, carrying out result analysis. According to the combined detection method, a direct fluorescence labeling cell surface antibody and nucleic acid technology is combined with conventional cell examination and other methods to carry out combined detection analysis, such that the combined detection method can be used for detecting a variety of diseases such as benign and malignant tumors, inflammations, bacteria, tuberculosis infection and the like, has a wide disease detection range, and has high clinical diagnosis and identification significance; and various body fluids such as urine, sputum, alveolar lavage fluid, pleuroperitoneal fluid and dialysate can be adopted for detection, materials are convenient and simple to obtain, and the cost is low.

Description

technical field [0001] The invention relates to the technical field of immune analysis, in particular to a combined detection method of flow cytometry in humoral cell immune analysis. Background technique [0002] The existing immunoassay technology adopts the method of obtaining the position coordinates of each cell in the flow sample in a coordinate system with the molecular weight of different antigens on the cell surface as coordinate axes; according to the position coordinates, the cells in the flow sample are divided into multiple cell groups; identifying the respective cell types of the plurality of cell groups; judging whether the position coordinates of the cells in each of the cell groups are within a preset range corresponding to the cell types of the cell groups; and when The judgment result is that the position coordinates of the cells in the cell group are not within the preset range corresponding to the cell type of the cell group, and the cell group is determ...

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Problems solved by technology

[0004] 1. At present, blood analysis lags behind in the process of disease diagnosis. The three detection methods (immunotyping, DNA ploidy, cytokines) in the occurrence and development of the disease, the abnormal results of body fluids are earlier and more valuable than blood manifestations ;

[0005] 2. At present, the three methods are independently detected, and there is a lack of means to comprehensively evaluate the disease, and it is impossible to efficiently and accurately determine what kind of disease it is, and the scope of diagnosis is small;

[0006] 3. Traditional pathological diagnosis requires surgery to take tissue biopsy, which is costly, risky, and causes great damage and pain to the patient. Fine needle aspiration is now used to take a small amount of puncture samples for analysis;

[0007] 4. Detection of body fluid cells under an ordinary microscope: the morphology is atypical, and the number and quality of cells are variable. Abnormal cells are easy to be missed or misjudged the nature of cells due to subjective consciousness

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