Experimental method for rabbit KOA model chondrocytes by adopting medicine scraping method

Abstract

The invention provides an experimental method for rabbit KOA model chondrocytes by adopting a medicine scraping method, which is characterized by comprising the following steps: 1, establishing a rabbit KOA model; 2, regularly smearing the knee joint of the rabbit with the berchemia lineate massage cream by adopting a medicine scraping method; 3, preparing an animal specimen; 4, performing morphological observation on cartilage tissue in the specimen; and 5, carrying out immunohistochemical detection on caspase-3, caspase-9 and XIAP proteins in the cartilage tissue. The research of the invention proves that the medicine scraping method can reduce the expression quantity of caspase-3 and caspase-9 in the rabbit articular cartilage of the K0A model and up-regulate the content of XIAP in cytobiology, so that the articular cartilage is protected from being damaged, and the joint senescence is delayed.

Description

technical field [0001] The invention relates to a research method for knee joint chondrocytes, in particular to an experimental method for treating rabbit KOA model chondrocytes by drug scraping. Background technique [0002] Osteoarthritis (OA) is a chronic, progressive, and degenerative clinical common and frequently-occurring disease. With the aging of the population, the incidence of OA is increasing year by year. According to domestic and foreign epidemiological literature, OA The prevalence rate is about 15%, and it is as high as 50% for those older than 60 years old, and the final disability rate reaches 53%. The World Health Organization (WHO) lists OA, cardiovascular disease, and cancer as the three major killers that endanger human health, which have brought heavy burdens to society and families. Among OA, knee osteoarthritis (KOA) is the most common disease. Because the pathogenesis of knee osteoarthritis is not clear, many clinical treatment methods cannot fund...

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Problems solved by technology

Among them, in the process of apoptosis mediated by mitochondria / cytochrome C, once the cells are damaged, the mitochondria will spontaneously release cytochrome C, and the precursors of Apaf-1 and caspase-9 will combine with it, so that caspase- 9 activation, and then activate caspase-3, and start the caspase cascade reaction, thereby inspiring the chain reaction of various signaling factors in the entire apoptotic pathway, leading to cell apoptosis and eventually knee joint degeneration occurrence of lesions

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