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Method for extracting L-homoserine from fermentation liquor

A technology of homoserine and fermentation liquid, which is applied in the direction of organic chemical methods, chemical instruments and methods, and the preparation of organic compounds, and can solve the problems of long treatment time, low product purity, and large acid-base consumption in electrodialysis technology

Inactive Publication Date: 2021-04-23
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Activated carbon decolorization will produce a large amount of waste carbon while retaining part of the fermentation broth to reduce product yield; ion exchange desalination will generate waste water and consume a large amount of acid and alkali; electrodialysis technology has disadvantages such as long processing time, low desalination rate, and low product purity. , so there is an urgent need for a new method for extracting L-homoserine

Method used

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  • Method for extracting L-homoserine from fermentation liquor
  • Method for extracting L-homoserine from fermentation liquor

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A method for extracting L-homoserine from fermentation broth, comprising the following steps:

[0028] Step 1. Fermentation broth pretreatment: Take 5L of fermentation broth from the upper tank, the concentration of homoserine is 35g / L, adjust the pH value of the fermentation broth to 4, add 20mg / L water-soluble chitosan flocculant, and heat to 60-80°C for treatment 20 minutes to promote the flocculation and precipitation of water-soluble miscellaneous proteins, the microfiltration membrane removes bacteria and flocs, and obtains 4.8L of fermentation broth clear liquid, using a roll-type ultrafiltration membrane separation system with a molecular weight cut-off of 0.5KDa and an operating pressure of 2.0MPa. Remove small molecular peptides and short-chain proteins in the fermentation liquid, and collect 4.7L of ultrafiltration membrane permeate;

[0029] Step 2. Decolorization: Pass the ultrafiltration membrane permeate collected in step 1 into a 5-inch activated carbon ...

Embodiment 2

[0040] A method for extracting L-homoserine from fermentation broth, comprising the following steps:

[0041]Step 1. Fermentation broth pretreatment: Take 5L fermentation broth from the upper tank, the concentration of homoserine is 35g / L, adjust the pH value of the fermentation broth to 5.8, add 80mg / L water-soluble chitosan flocculant, heat to 60-80°C for treatment For 40 minutes, promote the flocculation and precipitation of water-soluble miscellaneous proteins, microfiltration membranes remove bacteria and flocs, and obtain 5L of fermentation broth clear liquid, use roll-type ultrafiltration membrane separation system, the molecular weight cut-off is 10KDa, and the operating pressure is 4.0MPa. Remove small molecular peptides and short-chain proteins in the liquid serum, and collect 5L of ultrafiltration membrane permeate;

[0042] Step 2, decolorization: pass the ultrafiltration membrane permeate collected in step 1 into a 5-inch activated carbon fiber module device for d...

Embodiment 3

[0053] A method for extracting L-homoserine from fermentation broth, comprising the following steps:

[0054] Step 1. Fermentation broth pretreatment: Take 5L of fermentation broth from the upper tank, the concentration of homoserine is 40g / L, the pH of the fermentation broth is adjusted to 4.5, add 50mg / L water-soluble chitosan flocculant, and heat to 60-80°C for treatment For 30 minutes, the flocculation and precipitation of water-soluble miscellaneous proteins were promoted, and the bacteria and flocs were removed by the microfiltration membrane to obtain 4.9L of fermentation broth clear liquid. Using the roll-type ultrafiltration membrane separation system, the molecular weight cut-off was 5KDa, and the operating pressure was 3.0MPa. Remove small molecular peptides and short-chain proteins in the fermentation liquid, and collect 4.8L of ultrafiltration membrane permeate;

[0055] Step 2. Decolorization: Pass the ultrafiltration membrane permeate collected in step 1 into a ...

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Abstract

The invention relates to the field of L-homoserine extraction, in particular to a method for extracting L-homoserine from fermentation liquor. The method comprises the following steps of pretreatment of fermentation liquor, decoloration, electrodeionization, concentration and filtration to obtain L-homoserine. According to the method, the L-homoserine fermentation liquor is subjected to desalting treatment by adopting an electrodeionization technology for the first time, the desalting rate of the electrodeionization technology is increased by 10% compared with an electrodialysis technology, resin added in the electrodeionization technology does not need to be regenerated compared with an ion exchange technology, the use of a large amount of acid and alkali is reduced, and the water consumption can be saved by 70%.

Description

technical field [0001] The invention relates to the field of L-homoserine extraction, in particular to a method for extracting L-homoserine from fermentation broth. Background technique [0002] L-homoserine (2-amino-4-hydroxybutyric acid) is a non-protein amino acid, and its derivatives can not only be converted into other important chemical intermediates through enzymatic reactions, but also have rich biological activities, such as Can effectively suppress sickle red blood cells, antifungal drugs. L-homoserine is also an important intermediate for the synthesis of chiral herbicide-glufosinate-ammonium, so the research on L-homoserine in aspects of pharmacy and biology has been paid more and more attention by scientific researchers. [0003] At present, the methods for industrial production of L-homoserine are mainly chemical synthesis and biological methods; the chemical synthesis is mainly methionine method: by reacting with methyl iodide to form onium salt, then adding ...

Claims

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Application Information

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IPC IPC(8): C07C227/40C07C229/22
CPCC07B2200/07C07C227/40C07C229/22
Inventor 黄清荣冯志彬张娟张建龙于天池
Owner LUDONG UNIVERSITY
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