Antibacterial nano material with high biocompatibility and preparation method thereof, cell membrane extraction method and membrane-coated particle preparation method

A nanoparticle and nanomaterial technology, applied in the field of antibacterial materials, can solve the problems of reducing treatment efficiency and short blood retention time, and achieve high bactericidal effect and high biocompatibility

Pending Publication Date: 2021-04-30
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extremely short blood retention time severely reduces treatment efficiency

Method used

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  • Antibacterial nano material with high biocompatibility and preparation method thereof, cell membrane extraction method and membrane-coated particle preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Bacterial culture: Gram-positive Staphylococcus aureus (ATCC 25923) was cultured in Tryptone Broth (TSB). A single colony of Staphylococcus aureus on solid TSB agar plate was transferred to TSB liquid medium and cultured at 37°C for 16 hours. Then 10 μL of the overnight cultured bacterial suspension was resuspended in 1 mL of TSB medium, and grown at 37°C for 6 hours to obtain a bacterial suspension for use.

[0041] Preparation of antibacterial nanoparticles: under continuous stirring, 39.48 mg of selenium powder was dispersed in 100 mL of ultrapure water, and then 50 mg of sodium borohydride was added for reduction under nitrogen protection. Separately prepare 5 mL of CuCl 2 2H 2 O (42.62mg) dispersion and 5mL FeSO 4 ·7H 2 O (69.75mg) dispersion solution was simultaneously injected into the reduced selenium powder solution after 30 minutes. Collect CuFeSe by filtration through a 0.1 µm membrane filter 2 , freeze-dried to obtain CuFeSe 2 powder.

[0042] Cell m...

Embodiment 2

[0049] Bacterial culture: Gram-positive Staphylococcus aureus (ATCC 25923) was grown in nutrient broth (LB). A single colony of Staphylococcus aureus on solid LB agar plate was transferred to LB liquid medium and cultured at 37 °C for 16 h. Then resuspend 10 μL of overnight cultured bacterial suspension in 1 mL of LB medium, and grow at 37°C for 8 hours to obtain a bacterial suspension for use.

[0050] Preparation of antibacterial nanoparticles: Disperse 98.7 mg of selenium powder in 250 mL of ultrapure water under constant stirring, then add 125 mg of sodium borohydride and reduce it under nitrogen protection. Separately prepare 12.5 mL of CuCl 2 2H 2 O (106.55mg) dispersion and 12.5mL FeSO 4 ·7H 2 O (174.375mg) dispersion liquid was injected into the reduced selenium powder solution at the same time after 30 minutes. Collect CuFeSe by filtration through a 0.1 µm membrane filter 2 , freeze-dried to obtain CuFeSe 2 powder.

[0051] Cell membrane separation and extract...

Embodiment 3

[0056] Bacterial culture: Gram-positive Staphylococcus aureus (ATCC 25923) was grown in nutrient broth (LB). A single colony of Staphylococcus aureus on solid LB agar plate was transferred to LB liquid medium and cultured at 37 °C for 12 h. Then resuspend 10 μL of overnight cultured bacterial suspension in 1 mL of LB medium, and grow at 37°C for 6 hours to obtain a bacterial suspension for use.

[0057] Preparation of antibacterial nanoparticles: under continuous stirring, 40.23 mg of selenium powder was dispersed in 100 mL of ultrapure water, and then 50.19 mg of sodium borohydride was added for reduction under nitrogen protection. Separately prepare 5 mL of CuCl 2 2H 2 O (43.47mg) dispersion and 5mL FeSO 4 ·7H 2 O (68.11mg) dispersion solution was simultaneously injected into the reduced selenium powder solution after 30 minutes. Collect CuFeSe by filtration through a 0.1 µm membrane filter 2 , freeze-dried to obtain CuFeSe 2 powder.

[0058] Cell membrane separation...

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Abstract

The invention discloses an antibacterial nano material with high biocompatibility and a preparation method thereof, a cell membrane extraction method and a membrane-coated particle preparation method. The antibacterial nano material is nano-particles wrapped by a cell membrane, the nano-particles are copper iron selenide nano-particles (CuFeSe2), and the cell membrane is a dendritic cell membrane. The preparation method of the antibacterial nanoparticles mainly comprises the following steps: dispersing selenium powder and sodium borohydride powder into 100mL of ultrapure water according to a mass ratio of 1: 1.25-1.3 to obtain a solution A; preparing 0.25 + / -0.005 mmol of CuCl2. 2H2O solution and 0.25 + / -0.005 mmol of FeSO4. 7H2O solution respectively, and injecting the solutions into the solution obtained in the step A after 30 + / -2 minutes according to the volume ratio of 1: 1; and filtering and collecting CuFeSe2 nano particles, and carrying out freeze drying to obtain CuFeSe2 powder. The high biocompatibility and the high sterilization effect are realized.

Description

technical field [0001] The invention belongs to the technical field of antibacterial materials, and in particular relates to an antibacterial nanomaterial with high biocompatibility, a preparation method thereof, a cell membrane extraction method, and a preparation method of membrane-wrapped particles. Background technique [0002] According to the World Health Organization (WHO), most deaths by 2050 will be caused by drug-resistant bacteria, which poses a serious challenge to global health security. The emergence of super-resistant bacteria caused by overuse of antibiotics threatens human health; therefore, it is urgent to find new antimicrobial agents. [0003] Nanomaterials serve as antibiotic alternatives for bacterial infection treatment, yet only a few nanomaterial-based approaches are available for clinical applications, and their in vivo therapeutic efficiency remains low due to immune clearance and severe immune responses. After injecting the prepared nanomaterial-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K33/34A61K47/46A61P31/04C01B19/00B82Y40/00B82Y30/00
CPCA61K33/34A61K9/5176A61P31/04C01B19/002B82Y30/00B82Y40/00C01P2002/72C01P2004/04
Inventor 侯萱胡献刚迟雪曾辉
Owner NANKAI UNIV
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