Culture method of nitrifying bacteria

A technology of nitrifying bacteria and a culturing method, applied in the field of environmental microorganisms, can solve the problems of complex control process, inability to realize automatic feeding culture, etc., and achieve the effects of high bacterial activity and fast reproduction speed.

Active Publication Date: 2021-05-04
CHINA PETROLEUM & CHEM CORP +1
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can realize the screening and enrichment culture of nitrifying bacteria in a short period of ti

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Embodiment 1 Nitrifying bacteria culture

[0037]Nitrifying bacteria were cultivated in a 100L aeration reactor. The reactor is equipped with stirring, pH, feed automatic control system and dissolved oxygen concentration control system at the same time. During the cultivation process, the temperature was set at 32° C., and the pH was set at 7.6-7.7.

[0038] (1) First add 50L of microbial initial culture solution into the aeration reactor, turn on the aeration system without inoculating the microorganisms and adjust the aeration volume for aeration until the DO concentration no longer increases, thus determining the required amount of culture. The upper limit value DOmax of DO concentration and the corresponding aeration volume Q should be kept constant throughout the bacterial culture process. The composition and content of the microbial culture medium are: 50 g of ammonium sulfate, 1.5 g of ferrous sulfate, 0.5 g of calcium chloride, 7 g of magnesium sulfate heptahy...

Example Embodiment

[0042] Embodiment 2 Nitrifying bacteria culture

[0043] Nitrifying bacteria were cultivated in a 100L aeration reactor. The reactor is equipped with a stirring, pH, feeding automatic control system and a dissolved oxygen concentration control system at the same time. During the cultivation process, the temperature was set at 33° C., and the pH was set at 7.9-8.0.

[0044] (1) First add 50L of microbial initial culture solution into the aeration reactor, turn on the aeration system without inoculating the microorganisms and adjust the aeration volume for aeration until the DO concentration no longer increases, thus determining the required amount of culture. The upper limit value DOmax of DO concentration and the corresponding aeration volume Q should be kept constant throughout the bacterial culture process. The composition and content of the microbial culture solution are: 50 g of ammonium sulfate, 1.5 g of ferrous sulfate, 0.5 g of calcium chloride, 7 g of magnesium sulfa...

Example Embodiment

[0048] Embodiment 3 Nitrifying bacteria culture

[0049] Nitrifying bacteria were cultivated in a 100L aeration reactor. The reactor is equipped with a stirring, pH, feeding automatic control system and a dissolved oxygen concentration control system at the same time. During the cultivation process, the temperature was set at 35° C., and the pH was set at 8.0-8.1.

[0050] (1) First add 50L of microbial initial culture solution into the aeration reactor, turn on the aeration system without inoculating the microorganisms and adjust the aeration volume for aeration until the DO concentration no longer increases, thus determining the required amount of culture. The upper limit value DOmax of DO concentration and the corresponding aeration volume Q should be kept constant throughout the bacterial culture process. The composition and content of the microbial culture solution are: 50 g of ammonium sulfate, 1.5 g of ferrous sulfate, 0.5 g of calcium chloride, 7 g of magnesium sulfa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a culture method of nitrifying bacteria. The culture method comprises the following steps: (1) determining an upper limit value of dissolved oxygen concentration in a culture system as DOmax and a corresponding aeration rate Q; (2) determining a dissolved oxygen concentration in the optimal growth state period of the nitrifying bacteria as DOtop; and (3) in the culture process of the nitrifying bacteria, when the dissolved oxygen concentration in the culture system is increased to 35-60% DOmax, starting a feeding system, when the DO concentration is reduced by 10-30%, stopping the feeding system, and recording the dissolved oxygen concentration at the moment as DOs; after feeding is stopped, when the dissolved oxygen concentration in the culture system is reduced or increased by more than 3% compared with the DOs, starting a dissolved oxygen concentration regulation and control system, and stopping the dissolved oxygen concentration regulation and control system until the dissolved oxygen concentration is maintained at the DOtop; and repeating the steps until culture is finished. The method can realize automatic control culture of thalli and realize rapid growth and reproduction, and the obtained nitrifying bacteria have high activity and high ammonia nitrogen removal rate, and solve a problem of slow growth of bacterial sources required for industrial applications.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, in particular to a method for cultivating nitrifying bacteria. Background technique [0002] The main function of nitrifying bacteria is to remove nitrogenous pollutants, including ammonia oxidizing bacteria and nitrite oxidizing bacteria. Among them, ammonia oxidizing bacteria are responsible for oxidizing ammonia into nitrite, and nitrite oxidizing bacteria are responsible for further oxidizing nitrite into nitrate. Nitrifying bacteria are aerobic autotrophic microorganisms. Under the condition of sufficient supply, they can obtain energy from the process of oxidation of ammonia and nitrite and then assimilate CO 2 And growth, so its own growth is slow, the generation time is long, especially in the growth process, it is also easily affected by various factors such as substrate concentration, dissolved oxygen (DO) concentration, pH, etc., so the value-added speed is slow, and it is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12Q3/00
CPCC12N1/20C12Q3/00Y02W10/10
Inventor 高会杰王刚孙丹凤陈明翔李宝忠
Owner CHINA PETROLEUM & CHEM CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap