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Antibacterial peptide liquid composition and preparation thereof

A liquid composition, antibacterial peptide technology, applied in the direction of antibacterial drugs, drug combination, drug delivery, etc., to achieve the effect of wide application prospects

Pending Publication Date: 2021-05-07
JIANGSU PROTELIGHT PHARMA & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention aims to provide a liquid composition of antibacterial peptides, in order to solve the increasingly serious problem of drug resistance of bacteria and the pain caused by stubborn infections to the majority of patients

Method used

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  • Antibacterial peptide liquid composition and preparation thereof
  • Antibacterial peptide liquid composition and preparation thereof
  • Antibacterial peptide liquid composition and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, antimicrobial peptide composition stabilizer screening

[0049] Select mannitol, glycerol, lactose and glycine etc. as the stabilizing agent of antimicrobial peptide, prepare respectively the aqueous solution of antimicrobial peptide composition (antimicrobial peptide concentration is 2 ‰), investigate the stability of antimicrobial peptide composition, detection index comprises character, related substance ( The largest single impurity and total impurity), content, etc.

[0050] Related substance testing methods:

[0051] According to the high-performance liquid chromatography (Chinese Pharmacopoeia 2015 Edition, Appendix V D), using octadecylsilane-bonded silica gel as a filler, take 20 μL of the solution and inject it into a liquid chromatograph, and record the chromatogram. Relevant substances and limits: known impurities A, B, C, D and unknown single impurities, each impurity shall not exceed 1.0%, and the maximum simple impurities shall be summarize...

Embodiment 2

[0067] The molar concentration investigation of embodiment 2 antimicrobial peptide composition buffer system and buffer system

[0068] The antibacterial peptide composition buffer system is investigated according to the combination of the following table, and the mass concentration of the antimicrobial peptide in each antimicrobial peptide composition buffer system in the following table is 4‰.

[0069] Table 4 antimicrobial peptide composition buffer system

[0070]

[0071]

[0072] Table 5 antimicrobial peptide composition buffer system test investigation result

[0073]

[0074] It can be seen from Table 5 that the selection of disodium hydrogen phosphate and citric acid buffer system can ensure that the antimicrobial peptide composition solution can still have more than 98% of the antimicrobial peptide content in 3 months (at 25° C.). Therefore, disodium hydrogen phosphate and citric acid buffer system is the preferred buffer system.

[0075] For the preferred...

Embodiment 3

[0079] Embodiment 3, pH value investigation of antimicrobial peptide composition

[0080] Select mannitol (1%) as the stability, disodium hydrogen phosphate and citric acid as the buffer system (0.015M) to prepare different pH values ​​(3.0, 3.5, 4.5, 5.5, 6.5) antimicrobial peptide (2‰) composition solution , to investigate the stability of the antimicrobial peptide composition under different pH conditions, and the results are shown in Table 7.

[0081] Select hydrochloric acid simultaneously as pH regulator, adjust the pH value of antimicrobial peptide aqueous solution to 4.5, investigate the stability of antimicrobial peptide, and compare with disodium hydrogen phosphate and citric acid as the antimicrobial peptide composition solution of buffer system, the results are shown in the table 8.

[0082] The influence of table 7 composition pH value on antimicrobial peptide stability

[0083]

[0084] The influence (pH4.5) of different pH regulators of table 8 on the stabi...

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Abstract

The invention provides an antibacterial peptide composition. The amino acid sequence of the antibacterial peptide is KWKSFLKTFaAbKTVLHTALKAISS, and substitution sites of a and b are selected from any amino acids as follows: L-leucine, D-leucine, L-valine, D-valine, L-alanine, D-alanine, glycine, L-serine, D-serine, L-lysine and D-lysine. The composition comprises at least one stabilizer, antibacterial peptide and a buffer system (preferably disodium hydrogen phosphate and citric acid), and the pH value of the composition is 3.5-5.5. The antibacterial peptide composition is a broad-spectrum anti-infection drug for local external use, is suitable for various primary skin infections caused by pathogenic bacteria, especially drug-resistant bacteria, and secondary skin infections such as eczema combined infection and ulcer combined infection, including stubborn infectious diseases such as diabetic foot, burn wound infection and bedsore infection, and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an antimicrobial peptide liquid composition and its preparation. Background technique [0002] Antibacterial drugs mainly include antibiotics and synthetic antibacterial drugs. Since the 1920s and 1930s, the discovery and application of antibiotics has saved countless lives. Antibiotics are substances produced by bacteria or other microorganisms during their life, which can inhibit or kill pathogenic microorganisms such as bacteria, spirochetes, mycoplasma, and chlamydia. Antibiotics that have been on the market mainly include β-lactams, macrolides, polysaccharides (vancomycin, teicoplanin), aminoglycosides, tetracyclines, chloramphenicols, peptide lactones (up to totomycin), etc. [0003] If earlier humans benefited from it, the world today has to grapple with the consequences of antibiotic overuse - the emergence and spread of drug-resistant bacteria. Super bacteria are not sen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61K47/12A61K47/02A61K47/26A61K47/10A61K47/18A61K47/36A61K9/12A61P31/04A61P17/00A61P17/02
CPCA61K38/16A61K47/12A61K47/02A61K47/26A61K47/10A61K47/183A61K47/36A61K9/0014A61K9/12A61P31/04A61P17/00A61P17/02
Inventor 陈育新陈明侠陈文学黄晓雪
Owner JIANGSU PROTELIGHT PHARMA & BIOTECH
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