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A kind of preparation method of injectable self-healing antibacterial hydrogel

A self-healing, hydrogel technology, used in pharmaceutical formulations, prostheses, bandages, etc., can solve the problems of insignificant peeling efficiency, energy consumption, time-consuming, etc., and achieve excellent mechanical strength and biological activity, good biological phase. Capacitive and bioactive effects

Active Publication Date: 2022-06-14
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although sodium hydroxide and potassium hydroxide have similar chemical properties, silk must be cyclically frozen-thawed in sodium hydroxide / urea aqueous solution to effectively strip silk fibers to obtain nanofibers, which is not only time-consuming and energy-consuming, but also has low stripping efficiency. Obviously, there are still many micron-scale silk fibers that cannot be peeled off, so it is difficult for practical application

Method used

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  • A kind of preparation method of injectable self-healing antibacterial hydrogel
  • A kind of preparation method of injectable self-healing antibacterial hydrogel
  • A kind of preparation method of injectable self-healing antibacterial hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Prepare 98g of an aqueous solution containing 20wt% potassium hydroxide and 4wt% urea, pre-cool to -30°C, add 2g chitin at room temperature, and mechanically stir for 30 minutes to obtain a transparent and clear 2wt% chitin solution; add to the chitin solution 5.96g of 3-chloro-2-hydroxypropyltrimethylammonium chloride, so that the molar ratio of the quaternization reagent and the chitin unit is 4:1, the reaction temperature is 25 ° C, and HCl is added after stirring for 24 hours to neutralize the reaction The reaction solution was dialyzed with distilled water for 7 days, and then freeze-dried to obtain a white fibrous chitin quaternary ammonium salt sample. The obtained sample was measured for degree of substitution DQ=0.18, NH 2 Content = 15%.

[0024] The preparation methods of Examples 2 to 6 are similar to those of Example 1, and the differences are shown in Table 1 below.

[0025] Table 1 Examples 1-6 preparation parameters and the degree of substitution and NH ...

Embodiment 7

[0028] Prepare 98g of an aqueous solution containing 18wt% potassium hydroxide and 6wt% urea, pre-cool to -30°C, add 2g chitosan at room temperature, and mechanically stir for 30 minutes to obtain a transparent and clear 2wt% chitosan solution; 7.53g of 3-chloro-2-hydroxypropyltrimethylammonium chloride was added to the solution, so that the molar ratio of the quaternization agent and the chitosan unit was 4:1, the reaction temperature was -30 ° C, and after stirring for 24 hours HCl was added to neutralize the reaction solution; the reaction solution was dialyzed with distilled water for 7 days and freeze-dried to obtain a white fibrous chitosan quaternary ammonium salt sample (DQ=0.39, NH 2 = 78%).

[0029] The preparation methods of Examples 8 to 12 are similar to those of Example 7, and the differences are shown in Table 2 below.

[0030]Table 2 Examples 7-12 preparation parameters and the degree of substitution and NH of the obtained quaternary ammonium salt samples 2 c...

Embodiment 13

[0033] Prepare 98g of an aqueous solution containing 20wt% potassium hydroxide and 4wt% urea, pre-cool to -30°C, add 2g chitin at room temperature, and mechanically stir for 30 minutes to obtain a transparent and clear 2wt% chitin solution; add to the chitin solution 3-Chloro-2-hydroxypropyltrimethylammonium chloride, then add chloroacetic acid, so that the molar ratio of quaternization reagent and chitin unit is 4:1, so that the molar ratio of chloroacetic acid and chitin unit is 4 : 1, the reaction temperature was 40 ° C, and HCl was added to neutralize the reaction solution after stirring for 24 hours; the reaction solution was dialyzed with distilled water for 7 days, and freeze-dried to obtain a white fibrous quaternized carboxymethyl chitin sample, and the obtained sample was measured to replace Degree of substitution of quaternary ammonium salt DQ=0.18, degree of carboxymethyl substitution DS=0.15, NH 2 The content is 35%.

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Abstract

The invention relates to a preparation method of an injectable self-healing antibacterial hydrogel. Potassium hydroxide / urea aqueous solution is used as a solvent, and the natural silk is stripped into nanofibers after rapid stirring at room temperature and ultrasonic waves, and then separated to obtain uniform silk nanofiber solution. The silk nanofiber is selectively oxidized and mixed with chitin quaternary ammonium salt solution or chitosan quaternary ammonium salt solution to obtain an injectable self-healing antibacterial silk nanofiber / chitin or chitosan quaternary ammonium hydrogel. The obtained hydrogel has excellent biological activity and biodegradability at the same time.

Description

technical field [0001] The invention relates to a preparation method of an injectable self-healing antibacterial hydrogel, belonging to the fields of nanomaterials and biological application tissue engineering. Background technique [0002] In recent years, due to the reversible nature of dynamic chemistry, it has been widely used in gas adsorption and separation, nanocomposite preparation and construction of new materials, and has attracted more and more researchers' attention. In addition, dynamic chemistry has also been used to design and construct hydrogels with injectable or self-healing capabilities. Injectable hydrogels have a wide range of applications in drug loading, cell culture, and tissue engineering due to their outstanding advantages. In the reported construction of hydrogels suitable for cell encapsulation and biomedical tissue engineering, injectable hydrogels with self-healing ability have great advantages. [0003] Quaternized chitin and quaternized chito...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/50A61L27/54A61L27/26A61L26/00C08J3/075C08L89/00C08L5/08C08H1/00C08B37/08
CPCA61L27/52A61L27/50A61L27/54A61L27/26A61L26/008A61L26/0061A61L26/0066A61L26/0052C08J3/075C08H1/00C08B37/003A61L2400/06A61L2400/12A61L2300/232A61L2300/404A61L2300/602C08J2389/00C08J2305/08C08L89/00C08L5/08
Inventor 蔡杰谢芳陈毅军陆艺文
Owner WUHAN UNIV
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