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Hypsizigus marmoreus strain, spore, sporocarp and application thereof

A technology of shiitake mushrooms and strains, applied in the field of fruiting bodies and their applications, shiitake mushroom strains and spores, can solve the problems of long cultivation period of strains, unable to meet market demand, short fresh-keeping period, etc. Long-distance transportation and long shelf life

Active Publication Date: 2021-05-28
SHANGHAI FINC BIO TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of jitake mushroom strain, spore, fruiting body and application thereof, aiming at solving the problem of long cultivation period and fresh-keeping of bacterial strain in the prior art. The problem of being short-term and unable to meet market demand

Method used

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  • Hypsizigus marmoreus strain, spore, sporocarp and application thereof
  • Hypsizigus marmoreus strain, spore, sporocarp and application thereof
  • Hypsizigus marmoreus strain, spore, sporocarp and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The cross breeding of Finc-B-8031 described in embodiment 1

[0044] In this embodiment, the specific breeding method of the Finc-B-8031 is as follows:

[0045] 1) Select parental strains: select bacterial strain Beidou 18 and bacterial strain NN-12 as parents, wherein bacterial strain Beidou 18 and bacterial strain NN-12 are all from commercially available commodities;

[0046] 2) Prepare mother seed culture medium, original seed culture medium and cultivar culture medium: each culture medium formula is respectively:

[0047] Mother seed medium (PDA medium): 200 g of potato, 20 g of glucose and 20 g of agar are used for each 1 L of medium.

[0048] Original seed culture medium: including 38-40% original seed culture material and 60-62% water by mass percentage, the original seed culture material includes 55-70% sawdust, 15-20% rice bran, Bran 5-10% and corn flour 8-35%.

[0049] Cultivation culture medium: comprising 34-36% of cultivar compost and 64-66% of water by m...

Embodiment 2

[0052] Embodiment 2 Antagonism experiment

[0053] With the present invention jiji mushroom strain Finc-B-8031, bacterial strain Beidou No. 18 and bacterial strain NN-12 as test materials, the test method refers to NY / T 1845 edible fungus strain differential identification antagonistic reaction.

[0054] The strain Finc-B-8031 was inoculated and cultivated against the strain Beidou 18 and the strain NN-12 on the plate medium respectively. Please refer to Table 1 for the test results.

[0055] Table 1 Antagonism test among different strains of jijiji mushrooms

[0056] Variety Beidou 18 NN-12 Finc-B-8031 + +

[0057] Note: "+" indicates antagonism.

[0058] Antagonism experiments showed that the strain Finc-B-8031 was different from the strain Beidou No. 18 and the strain NN-12, which was different from the strain of Shiji mushroom.

Embodiment 3

[0059] Embodiment 3 culture cycle and fresh-keeping experiment

[0060] Because the jitake mushroom strain Finc-B-8031 of the present invention is a crab-flavored mushroom, so this embodiment compares the cultivation cycle, per unit yield, and fresh-keeping period with the most widely planted NN-12 in the existing crab-flavored mushroom varieties, and summarizes the results of this study. The advantages and characteristics of the invented jiji mushroom strain Finc-B-8031.

[0061] The cultivation conditions that present embodiment adopts are as follows:

[0062] See cultivar culture medium for culture medium, no more details.

[0063] Cultivation bottle volume: 850mL.

[0064] Culture conditions: temperature 21~27℃, humidity 75%, CO 2 The concentration is 2500-4000ppm. The culture period is the number of days from inoculation to scratch bacteria.

[0065] Cultivation conditions: the temperature of the cultivation room is set at 13-17°C, the humidity is 92-99%, CO 2 The c...

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Abstract

The invention provides a hypsizigus marmoreus strain, spore and sporocarp and application thereof, the strain is named as Finc-B-8031, belongs to hypsizigus marmoreus and is preserved in China General Microbiological Culture Collection Center, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.21448, and the preservation date is January 5, 2021. The hypsizigus marmoreus strain provided by the invention solves the problems that in the prior art, the strain is long in cultivation period and short in fresh-keeping period, and the market demand cannot be met.

Description

technical field [0001] The invention belongs to the field of edible fungi, and relates to a strain, spores, fruiting bodies and applications of jiji mushrooms. Background technique [0002] True Ji mushroom (Hypsizygus marmoreus (Peck) Bigelow) belongs to Basidiomycotina, Phytomycetes, Agaricaceae, White Mushrooms, and Amygium genus. Because it has a unique crab flavor, it is also called crab-flavored mushroom, seafood mushroom, etc. The current Shiji mushrooms produced in factories mainly contain two kinds of brown and pure white. Generally speaking, brown Shiji mushrooms are crab-flavored mushrooms, and white Shiji mushrooms are white jade mushrooms. [0003] The nutrients contained in the mushroom are rich and comprehensive, and have anti-hypertensive, anti-cancer, anti-oxidation and other effects. Because it is rich in vitamins, amino acids, minerals, etc., it has high nutritional value and delicious taste, and is deeply loved by consumers. [0004] Different enterpri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L33/00A23L31/00C12R1/645
CPCC12N1/14A23L33/00A23L31/00
Inventor 刘玉王楠
Owner SHANGHAI FINC BIO TECH INC
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