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A method for raising sugarcane borer or two-spotted borer indoors

A technology of sugarcane strip borer and two-point borer, which is applied in animal feed, animal feed, animal husbandry and other directions, can solve the problems of extensive feeding process, low feeding rate of larvae, targeted feeding of small and large insects, etc. The effect of food competition, the improvement of survival rate, and the effect of high nutritional value

Active Publication Date: 2022-04-22
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another example is the patent for invention with the application number CN201710843705, with a publication date of 20180105, disclosing an artificial feed for C. mori and its preparation method. The artificial feed for C. moer is composed of wheat germ powder, yeast powder, casein, and sugarcane shoot powder. , choline chloride, distilled water and other more than 10 kinds of raw materials, adopting the feeding method of corn in the early stage and formula feed in the later stage, although the survival rate of larvae has been improved to a certain extent, the feeding process is extensive, and neither small insects nor large insects are targeted. The feeding rate of larvae is low, and the survival rate and eclosion rate still need to be improved. Therefore, it is imperative to study the method of large-scale and high-efficiency indoor breeding of sugarcane borer and two-spotted borer.

Method used

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  • A method for raising sugarcane borer or two-spotted borer indoors
  • A method for raising sugarcane borer or two-spotted borer indoors
  • A method for raising sugarcane borer or two-spotted borer indoors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A kind of method of indoor raising sugarcane bar borer or two point borer, comprises the following steps:

[0049] S1. Disinfection of egg masses: cut off the egg masses of blackheads, wash them in distilled water, and then put them in disinfectant water (including 2% sodium hypochlorite and 2% formaldehyde) for 5 minutes, take them out and put them in distilled water for cleaning. Blot the water on the egg mass with sterile filter paper, and place it on the aseptic operating table for later use.

[0050] S2. Larva rearing: Adopt the 2-10-20-25 mode: put the egg masses of blackheads into the petri dish and take out the egg shells and filter paper on the second day; increase the worm feed in the petri dish on the tenth day; change a single tube on the 20th day And new big worm feed; Add folded filter paper for pupation on the 25th day.

[0051] The specific operation is as follows:

[0052] Take the prepared worm feed out of the refrigerator and place it for more than ...

Embodiment 2

[0067] S1. Disinfection of egg masses: Cut off the egg masses of blackheads, put them in distilled water for cleaning, and then put them in disinfected water for 5 minutes, take them out and put them in distilled water for cleaning. Standby on the sterile operating table.

[0068] S2. Larva rearing: adopt the 3-12-22-25 mode: that is, put the blackhead egg mass into the petri dish and take out the eggshell and filter paper on the 3rd day; increase the worm feed in the petri dish on the 12th day; change the order on the 22nd day tube and new worm feed; add folded filter paper for pupation on the 25th day.

[0069] The worm feed is made of raw materials according to the following proportions:

[0070] Agar powder 15.0g, fresh sweet corn kernels 250.0g, sugarcane stem powder 40.0g, casein 15.0g, whole egg powder 15.0g, yeast powder 20.0g, sucrose 10.0g, cholesterol 0.3g, sodium chloride 0.2g, ascorbic acid 2.0 g, methylparaben 1.5g, sorbic acid 2.0g, multi-dimensional element t...

experiment example

[0080] 1.1 Insect sources:

[0081] The mature larvae of overwintering generations of sugarcane stem borer and two-spotted stem borer were collected from the seriously damaged sugarcane area in Chongzuo City, Guangxi Zhuang Autonomous Region, and brought back indoors for rearing. The larvae were reared with corn cobs in insect cages. After pupation, they emerged in insect cages and mate freely. They laid eggs on sugarcane leaves. After the egg masses hatched into larvae, they were reared circularly, and they were reared for 2 generations for later use.

[0082] Instrument: RXZ-280B intelligent artificial climate box, the set temperature is (26±1)°C, the humidity is (75±5)%, the photoperiod is 16L:8D, the light intensity is 9000lx, manufactured by Ningbo Jiangnan Instrument Factory.

[0083] 1.2 Experimental group:

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Abstract

The invention discloses a method for indoor feeding of sugarcane stripe borer or two-spotted borer. S1. egg mass disinfection; S2. larval feeding: first put small insect feed, take out the egg shell and filter paper on the 2nd to 3rd day; 9th to 12th day On the 18th to 22nd day, the feed for large insects was added, and the test tube was changed to a single head, and the insect feed was amplified again. On the 23rd to 26th day, a folded filter paper was added for pupation; the small insect feed and the large insect feed included agar powder, fresh sweet corn kernels , sugarcane stem powder, casein, whole egg powder, yeast powder, sucrose, cholesterol, sodium chloride, ascorbic acid, methylparaben, sorbic acid, multivitamin tablets, 40% v / v formaldehyde and distilled water, the formula ingredients are Different ratios are prepared separately. S3. pupa treatment; S4. adult paired oviposition; S5. cyclic feeding. The feeding method is beneficial to the growth needs of larvae and the needs of characteristics such as pupation, emergence, and egg laying, and can better meet the nutritional needs of the worms and is beneficial to the growth and development of the worms.

Description

【Technical field】 [0001] The invention relates to the technical field of insect feeding, in particular to a method for indoor feeding of the sugarcane barworm or the two-spotted borer. 【Background technique】 [0002] Sugarcane bar borer (Chilo sacchariphagus Bjojer) belongs to Lepidoptera moth family insects, is one of the most serious pests on sugarcane, causing great economic losses to my country's sugar industry every year, mainly distributed in Guangdong, Guangxi, Yunnan , Hainan, Fujian, Sichuan, Jiangxi and other provinces and regions, in addition to harming sugarcane, it can also harm crops such as sorghum and corn. Chilo infuscatellus Snellen, also known as millet ash borer, belongs to the family Pyralidae of the order Lepidoptera. Borer is widely distributed and occurs in India, Pakistan, Myanmar, the Philippines, and all areas where sugarcane is planted in my country. The larvae of Borer have mixed feeding habits. They mainly damage sugarcane in the south, millet i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A23K50/90A23K10/30A23K20/163A23K20/147A23K10/20A23K10/18A23K20/105A23K20/22A23K20/174A23K20/111
CPCA01K67/033A23K50/90A23K10/30A23K20/163A23K20/147A23K10/20A23K10/18A23K20/105A23K20/22A23K20/174A23K20/111
Inventor 魏吉利商显坤潘雪红黄诚华马永林高学花
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI