Bionic anti-tumor nano-drug and preparation method thereof
An anti-tumor drug and nano-drug technology, applied in the field of anti-tumor drugs, can solve problems such as limiting the probability of anti-tumor nano-drugs entering the tumor center, the transport efficiency of anti-tumor nano-drugs not exceeding 1%, and limiting clinical therapeutic effects and applications. , to achieve good anti-tumor effect, high delivery efficiency, good biological safety effect
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Embodiment 1
[0031] 20g melamine (purchased from Aladdin Reagent Company) was roasted at 550°C for 2 hours to obtain white g-C 3 N 4 , and then roasted at 550°C for 2h to obtain light yellow g-C 3 N 4 Precursor, finally calcined at 550°C for 2h to obtain white g-C 3 N 4 nanoflakes, followed by photodeposition of Pt on g-C 3 N 4 Surface made g-C 3 N 4 -Pt nanosheets. 1 mg gray g-C 3 N 4 - Pt flakes were dispersed under pH 7.4 phosphate buffer and ultrasonic conditions for 30 minutes, 1 mg of paclitaxel was added to the above solution and mixed, and then 1 mL of bisoxalate sodium bicarbonate was slowly added to the mixed solution under magnetic stirring at room temperature solution (0.5mg / mL), to obtain paclitaxel / g-C 3 N 4 - Pt / bisoxalate (PCC) nanosheets. After 1 hour, the cell membranes of macrophages and PCC were shaken in an air bath at 40°C for 15 hours. The obtained biomimetic anti-tumor nanomedicine (PCC@M) was resuspended in pH7.4 phosphate buffer, extruded 15 times wi...
Embodiment 2
[0034] Roast 25g of melamine at 400°C for 4 hours to obtain white g-C 3 N 4 , and then roasted at 400°C for 2h to obtain light yellow g-C 3 N 4 Precursor, finally calcined at 400°C for 2h to obtain white g-C 3 N 4 nanoflakes, followed by photodeposition of Pt on g-C 3 N 4 Surface made g-C 3 N 4 -Pt nanosheets. 1mg gray g-C 3 N 4 - Pt flakes were dispersed in phosphate buffer and ultrasound for 30 minutes, 1 mg of paclitaxel was added to the above solution and mixed, then 2 mL of luminol solution (0.8 mg / mL) was slowly added to the mixed solution at room temperature under magnetic stirring, get paclitaxel / g-C 3 N 4 - Pt / luminol nanosheets (PCL). After 1 hour, the macrophage membranes and PCL were shaken in an air bath at 40 °C for 12 hours. Resuspend the obtained biomimetic nanomedicine with phosphate buffer, extrude it 15 times with a liposome extruder, centrifuge at 10,000 rpm for 1 hour, take the precipitate and resuspend it with phosphate buffer to obtain PCL@...
Embodiment 3
[0037] Roast 25g of melamine at 550°C for 4 hours to obtain white g-C 3 N 4 , and then roasted at 500°C for 2h to obtain light yellow g-C 3 N 4 Precursor, finally calcined at 500°C for 2h to obtain white g-C 3 N 4 nanoflakes, followed by photodeposition of Pt on g-C 3 N 4 Surface made g-C 3 N 4 -Pt nanosheets. 1mg gray g-C 3 N 4 -Pt flakes were dispersed in phosphate buffer and ultrasonic for 30 minutes, 1 mg of paclitaxel was added to the above solution and mixed, and then 1 mL of bisoxalate sodium bicarbonate solution (0.3 mg / mL), to get paclitaxel / g-C 3 N 4 -Pt / bisoxalate nanosheets. After 1 hour, shake the macrophage membrane and PCC in an air bath at 40°C for 12 hours. The resulting biomimetic anti-tumor nanomedicine (PCC@M) was resuspended in phosphate buffer, then extruded 15 times with a liposome extruder, centrifuged at 10,000rpm for 1 hour, and the precipitate was resuspended in phosphate buffer , stored at 4°C.
[0038] The water-splitting performan...
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