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DNA origami mineralization method capable of realizing responsive degradation

A technology of origami structure and ammonium chloride, which is applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of difficult application in biomedical fields and single application range, and achieve mineralization protection and rigidity improvement, Easy to operate and achieve the effect of responsive degradation

Pending Publication Date: 2021-06-04
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems in the prior art that mineralization using TEOS as a silicon source is difficult to apply to the biomedical field, and the application range is single, and proposes a method for mineralization that can respond to degradable DNA origami

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  • DNA origami mineralization method capable of realizing responsive degradation
  • DNA origami mineralization method capable of realizing responsive degradation
  • DNA origami mineralization method capable of realizing responsive degradation

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Embodiment 1

[0035] A degradable DNA origami mineralization method of the present invention comprises the following steps: (1) synthesizing the origami structure, further purifying and concentrating using PEG centrifugal technology;

[0036] step1 synthesis and purification of origami triangles; step2 dispersion and concentration of origami triangles;

[0037] step1 Synthesis and purification of origami triangles:

[0038]Take the PCR tube, add 59.2 microliters of pure water, 10 microliters of 125 millimolar magnesium salt prepared, 10 microliters of the M13 long chain from the phage at a concentration of 100 nanomoles per liter, and 20.8 microliters of the premixed fixed chain, The final system is 100 microliters. Put the above-mentioned PCR tubes into the PCR to achieve a gradient cooling from 90 degrees Celsius to 20 degrees Celsius. After two hours of assembly, take out the PCR tubes; Use a pipette gun to transfer to a 1 ml centrifuge tube, a total of 300 microliters of origami soluti...

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Abstract

The invention discloses a DNA origami mineralization method capable of realizing responsive degradation. The method comprises the following steps: (1) synthesizing an origami structure, and carrying out further purification and concentration by using a PEG (Polyethylene Glycol) centrifugal technology; (2) adding trimethyl[3-(trimethoxysilyl) propyl]ammonium chloride (TMAPS) and bis[3-(triethoxysilyl) propyl] tetrasulfide (BTES) to realize effective mineralization of the origami; and (3) conducting responsive degradation capability test. In order to realize that the origami plays a role in the field of biomedicine such as drug loading, the method is simple and convenient to operate, and compared with mineralization by using tetraethyl orthosilicate (TEOS) in literatures, the method can realize responsive rapid degradation of glutathione (GSH), and possibility is provided for the origami in the aspects of drug loading, catalysis and the like in the future.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a mineralization method capable of responding to degradable DNA origami. Background technique [0002] DNA origami mineralization technology is a technology that improves the rigidity of the DNA origami structure and improves the resistance of the origami to the external environment, thereby realizing bionics and simulations at the nanoscale. First of all, DNA origami is a technology that uses a DNA chain with a length of several thousand bases to fold into any shape under the action of a series of short fixed chains using complementary base pairing. Utilizing this technology can provide a variety of customized 2D and 3D nanocomponents with controllable size and shape. The great advantage of programmability makes DNA origami an outstanding advantage in the field of nanotechnology. DNA origami mineralization technology, that is, coating a layer of silica technology on the origami sk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H23/00C07H1/00
CPCC07H23/00C07H1/00
Inventor 田野刘鹏闵乾昊徐子棋胡晓雪戴李知
Owner NANJING UNIV
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